Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_28000

Experiment: Nitrate reduction with yeast extract

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_27995 and AO356_28000 are separated by 147 nucleotidesAO356_28000 and AO356_28005 are separated by 113 nucleotides AO356_27995: AO356_27995 - thioredoxin reductase, at 6,390,998 to 6,391,891 _27995 AO356_28000: AO356_28000 - Rrf2 family transcriptional regulator, at 6,392,039 to 6,392,506 _28000 AO356_28005: AO356_28005 - malate:quinone oxidoreductase, at 6,392,620 to 6,394,263 _28005 Position (kb) 6392 6393Strain fitness (log2 ratio) -2 -1 0 1 2 3at 6391.482 kb on + strand, within AO356_27995at 6391.482 kb on + strand, within AO356_27995at 6391.808 kb on + strandat 6391.809 kb on - strandat 6391.809 kb on - strandat 6391.809 kb on - strandat 6391.857 kb on + strandat 6391.858 kb on - strandat 6391.945 kb on - strandat 6392.192 kb on + strand, within AO356_28000at 6392.192 kb on + strand, within AO356_28000at 6392.193 kb on - strand, within AO356_28000at 6392.519 kb on + strandat 6392.622 kb on - strandat 6392.741 kb on + strandat 6392.772 kb on + strandat 6392.792 kb on + strand, within AO356_28005at 6393.063 kb on - strand, within AO356_28005at 6393.063 kb on - strand, within AO356_28005at 6393.119 kb on + strand, within AO356_28005at 6393.134 kb on + strand, within AO356_28005at 6393.204 kb on - strand, within AO356_28005at 6393.414 kb on - strand, within AO356_28005

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction with yeast extract
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6,391,482 + AO356_27995 0.54 -0.9
6,391,482 + AO356_27995 0.54 -0.8
6,391,808 + +0.1
6,391,809 - +0.2
6,391,809 - +0.1
6,391,809 - +2.1
6,391,857 + -0.4
6,391,858 - +0.2
6,391,945 - +1.1
6,392,192 + AO356_28000 0.33 -2.0
6,392,192 + AO356_28000 0.33 +1.2
6,392,193 - AO356_28000 0.33 +1.1
6,392,519 + -0.2
6,392,622 - -0.4
6,392,741 + -0.7
6,392,772 + -1.1
6,392,792 + AO356_28005 0.10 -0.0
6,393,063 - AO356_28005 0.27 +0.2
6,393,063 - AO356_28005 0.27 +0.2
6,393,119 + AO356_28005 0.30 +3.0
6,393,134 + AO356_28005 0.31 -1.0
6,393,204 - AO356_28005 0.36 -0.0
6,393,414 - AO356_28005 0.48 +0.0

Or see this region's nucleotide sequence