Experiment: Nitrate reduction with yeast extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_26945 and AO356_26950 are separated by 166 nucleotides AO356_26950 and AO356_26955 overlap by 4 nucleotides
AO356_26945: AO356_26945 - hypothetical protein, at 6,134,580 to 6,135,191
_26945
AO356_26950: AO356_26950 - ATP-dependent DNA ligase, at 6,135,358 to 6,137,970
_26950
AO356_26955: AO356_26955 - carboxylate--amine ligase, at 6,137,967 to 6,139,130
_26955
Position (kb)
6135
6136
6137
6138 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6134.501 kb on - strand at 6134.501 kb on - strand at 6134.667 kb on + strand, within AO356_26945 at 6134.809 kb on - strand, within AO356_26945 at 6134.916 kb on + strand, within AO356_26945 at 6134.916 kb on + strand, within AO356_26945 at 6134.917 kb on - strand, within AO356_26945 at 6134.917 kb on - strand, within AO356_26945 at 6134.917 kb on - strand, within AO356_26945 at 6134.917 kb on - strand, within AO356_26945 at 6134.917 kb on - strand, within AO356_26945 at 6134.919 kb on - strand, within AO356_26945 at 6135.075 kb on + strand, within AO356_26945 at 6135.076 kb on - strand, within AO356_26945 at 6135.110 kb on - strand, within AO356_26945 at 6135.153 kb on + strand at 6135.168 kb on + strand at 6135.189 kb on + strand at 6135.287 kb on - strand at 6135.360 kb on - strand at 6135.360 kb on - strand at 6135.402 kb on + strand at 6135.402 kb on + strand at 6135.605 kb on + strand at 6135.799 kb on + strand, within AO356_26950 at 6135.799 kb on + strand, within AO356_26950 at 6135.800 kb on - strand, within AO356_26950 at 6136.160 kb on + strand, within AO356_26950 at 6136.160 kb on + strand, within AO356_26950 at 6136.160 kb on + strand, within AO356_26950 at 6136.161 kb on - strand, within AO356_26950 at 6136.161 kb on - strand, within AO356_26950 at 6136.602 kb on + strand, within AO356_26950 at 6136.763 kb on + strand, within AO356_26950 at 6136.763 kb on + strand, within AO356_26950 at 6136.763 kb on + strand, within AO356_26950 at 6136.763 kb on + strand, within AO356_26950 at 6136.764 kb on - strand, within AO356_26950 at 6136.764 kb on - strand, within AO356_26950 at 6136.764 kb on - strand, within AO356_26950 at 6136.822 kb on + strand, within AO356_26950 at 6136.956 kb on - strand, within AO356_26950 at 6136.973 kb on + strand, within AO356_26950 at 6136.973 kb on + strand, within AO356_26950 at 6136.973 kb on + strand, within AO356_26950 at 6136.974 kb on - strand, within AO356_26950 at 6136.974 kb on - strand, within AO356_26950 at 6137.219 kb on + strand, within AO356_26950 at 6137.822 kb on + strand at 6137.823 kb on - strand at 6138.474 kb on + strand, within AO356_26955 at 6138.474 kb on + strand, within AO356_26955 at 6138.475 kb on - strand, within AO356_26955 at 6138.475 kb on - strand, within AO356_26955 at 6138.632 kb on - strand, within AO356_26955 at 6138.787 kb on + strand, within AO356_26955 at 6138.788 kb on - strand, within AO356_26955 at 6138.929 kb on - strand, within AO356_26955
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction with yeast extract remove 6,134,501 - +1.7 6,134,501 - +1.0 6,134,667 + AO356_26945 0.14 -1.1 6,134,809 - AO356_26945 0.37 +0.9 6,134,916 + AO356_26945 0.55 -1.4 6,134,916 + AO356_26945 0.55 +0.1 6,134,917 - AO356_26945 0.55 +1.2 6,134,917 - AO356_26945 0.55 -1.8 6,134,917 - AO356_26945 0.55 -2.4 6,134,917 - AO356_26945 0.55 +1.2 6,134,917 - AO356_26945 0.55 -0.1 6,134,919 - AO356_26945 0.55 +1.7 6,135,075 + AO356_26945 0.81 +1.3 6,135,076 - AO356_26945 0.81 -0.9 6,135,110 - AO356_26945 0.87 -2.9 6,135,153 + -1.4 6,135,168 + +1.7 6,135,189 + +1.8 6,135,287 - +0.2 6,135,360 - +0.7 6,135,360 - +1.4 6,135,402 + +1.8 6,135,402 + -0.7 6,135,605 + +1.4 6,135,799 + AO356_26950 0.17 -2.1 6,135,799 + AO356_26950 0.17 +2.1 6,135,800 - AO356_26950 0.17 +0.8 6,136,160 + AO356_26950 0.31 +0.9 6,136,160 + AO356_26950 0.31 +0.3 6,136,160 + AO356_26950 0.31 -2.2 6,136,161 - AO356_26950 0.31 +0.2 6,136,161 - AO356_26950 0.31 -1.7 6,136,602 + AO356_26950 0.48 -0.1 6,136,763 + AO356_26950 0.54 -0.2 6,136,763 + AO356_26950 0.54 +0.9 6,136,763 + AO356_26950 0.54 +0.7 6,136,763 + AO356_26950 0.54 +2.6 6,136,764 - AO356_26950 0.54 -1.8 6,136,764 - AO356_26950 0.54 +0.6 6,136,764 - AO356_26950 0.54 +0.3 6,136,822 + AO356_26950 0.56 -1.6 6,136,956 - AO356_26950 0.61 -1.5 6,136,973 + AO356_26950 0.62 +0.4 6,136,973 + AO356_26950 0.62 -2.0 6,136,973 + AO356_26950 0.62 +2.2 6,136,974 - AO356_26950 0.62 -2.0 6,136,974 - AO356_26950 0.62 +1.4 6,137,219 + AO356_26950 0.71 +0.2 6,137,822 + +1.4 6,137,823 - +1.0 6,138,474 + AO356_26955 0.44 +1.3 6,138,474 + AO356_26955 0.44 -0.5 6,138,475 - AO356_26955 0.44 +1.2 6,138,475 - AO356_26955 0.44 +1.7 6,138,632 - AO356_26955 0.57 -0.0 6,138,787 + AO356_26955 0.70 +0.2 6,138,788 - AO356_26955 0.71 -1.7 6,138,929 - AO356_26955 0.83 +0.8
Or see this region's nucleotide sequence