Experiment: Nitrate reduction with yeast extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25415 and AO356_25420 are separated by 236 nucleotides AO356_25420 and AO356_25425 are separated by 149 nucleotides AO356_25425 and AO356_25430 are separated by 5 nucleotides
AO356_25415: AO356_25415 - pyridine nucleotide-disulfide oxidoreductase, at 5,748,146 to 5,749,348
_25415
AO356_25420: AO356_25420 - chemotaxis protein CheY, at 5,749,585 to 5,749,959
_25420
AO356_25425: AO356_25425 - AraC family transcriptional regulator, at 5,750,109 to 5,750,927
_25425
AO356_25430: AO356_25430 - phosphatidylethanolamine-binding protein, at 5,750,933 to 5,751,472
_25430
Position (kb) 5749
5750 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5748.626 kb on - strand, within AO356_25415 at 5748.928 kb on + strand, within AO356_25415 at 5749.044 kb on + strand, within AO356_25415 at 5749.108 kb on + strand, within AO356_25415 at 5749.128 kb on - strand, within AO356_25415 at 5749.128 kb on - strand, within AO356_25415 at 5749.128 kb on - strand, within AO356_25415 at 5749.181 kb on - strand, within AO356_25415 at 5749.181 kb on - strand, within AO356_25415 at 5749.181 kb on - strand, within AO356_25415 at 5749.336 kb on + strand at 5749.336 kb on + strand at 5749.337 kb on - strand at 5749.337 kb on - strand at 5749.337 kb on - strand at 5749.337 kb on - strand at 5749.337 kb on - strand at 5749.337 kb on - strand at 5749.337 kb on - strand at 5749.337 kb on - strand at 5749.388 kb on + strand at 5749.480 kb on - strand at 5749.480 kb on - strand at 5749.518 kb on - strand at 5749.518 kb on - strand at 5749.518 kb on - strand at 5749.518 kb on - strand at 5749.518 kb on - strand at 5749.521 kb on + strand at 5749.521 kb on + strand at 5749.521 kb on + strand at 5749.521 kb on + strand at 5749.521 kb on + strand at 5749.522 kb on - strand at 5749.522 kb on - strand at 5749.522 kb on - strand at 5749.522 kb on - strand at 5749.522 kb on - strand at 5749.522 kb on - strand at 5749.522 kb on - strand at 5749.522 kb on - strand at 5749.522 kb on - strand at 5749.522 kb on - strand at 5749.536 kb on + strand at 5749.557 kb on + strand at 5749.557 kb on + strand at 5749.558 kb on - strand at 5749.560 kb on - strand at 5749.859 kb on + strand, within AO356_25420 at 5749.860 kb on - strand, within AO356_25420 at 5750.017 kb on + strand at 5750.018 kb on - strand at 5750.042 kb on + strand at 5750.043 kb on - strand at 5750.043 kb on - strand at 5750.043 kb on - strand at 5750.044 kb on + strand at 5750.045 kb on - strand at 5750.146 kb on + strand at 5750.147 kb on - strand at 5750.147 kb on - strand at 5750.336 kb on + strand, within AO356_25425 at 5750.526 kb on - strand, within AO356_25425 at 5750.526 kb on - strand, within AO356_25425 at 5750.580 kb on + strand, within AO356_25425 at 5750.580 kb on + strand, within AO356_25425 at 5750.580 kb on + strand, within AO356_25425 at 5750.580 kb on + strand, within AO356_25425 at 5750.580 kb on + strand, within AO356_25425 at 5750.581 kb on - strand, within AO356_25425 at 5750.625 kb on - strand, within AO356_25425 at 5750.625 kb on - strand, within AO356_25425 at 5750.642 kb on + strand, within AO356_25425 at 5750.683 kb on + strand, within AO356_25425 at 5750.683 kb on + strand, within AO356_25425 at 5750.683 kb on + strand, within AO356_25425 at 5750.684 kb on - strand, within AO356_25425 at 5750.871 kb on + strand at 5750.872 kb on - strand at 5750.907 kb on + strand at 5750.907 kb on + strand at 5750.907 kb on + strand at 5750.907 kb on + strand at 5750.908 kb on - strand at 5750.908 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction with yeast extract remove 5,748,626 - AO356_25415 0.40 +0.4 5,748,928 + AO356_25415 0.65 +0.4 5,749,044 + AO356_25415 0.75 +1.3 5,749,108 + AO356_25415 0.80 -1.4 5,749,128 - AO356_25415 0.82 +1.7 5,749,128 - AO356_25415 0.82 +1.8 5,749,128 - AO356_25415 0.82 -1.7 5,749,181 - AO356_25415 0.86 +2.2 5,749,181 - AO356_25415 0.86 -2.2 5,749,181 - AO356_25415 0.86 +0.7 5,749,336 + +0.9 5,749,336 + -0.2 5,749,337 - -0.5 5,749,337 - -2.0 5,749,337 - +0.1 5,749,337 - +0.5 5,749,337 - +2.2 5,749,337 - +1.0 5,749,337 - +0.9 5,749,337 - +2.8 5,749,388 + -1.8 5,749,480 - -1.0 5,749,480 - +0.8 5,749,518 - -1.3 5,749,518 - +1.0 5,749,518 - +0.1 5,749,518 - -1.9 5,749,518 - -1.8 5,749,521 + -1.2 5,749,521 + +0.7 5,749,521 + -1.5 5,749,521 + +0.5 5,749,521 + -0.9 5,749,522 - -1.0 5,749,522 - +0.3 5,749,522 - -1.4 5,749,522 - -0.7 5,749,522 - +1.8 5,749,522 - +1.0 5,749,522 - -0.5 5,749,522 - -0.6 5,749,522 - -0.4 5,749,522 - +1.6 5,749,536 + -1.4 5,749,557 + +0.6 5,749,557 + +0.2 5,749,558 - -1.6 5,749,560 - -1.6 5,749,859 + AO356_25420 0.73 +0.3 5,749,860 - AO356_25420 0.73 -0.5 5,750,017 + +0.8 5,750,018 - -0.2 5,750,042 + -1.9 5,750,043 - -1.2 5,750,043 - -1.6 5,750,043 - +0.6 5,750,044 + +0.8 5,750,045 - +0.4 5,750,146 + +0.6 5,750,147 - +2.8 5,750,147 - -0.4 5,750,336 + AO356_25425 0.28 +1.2 5,750,526 - AO356_25425 0.51 +0.7 5,750,526 - AO356_25425 0.51 -0.5 5,750,580 + AO356_25425 0.58 -0.2 5,750,580 + AO356_25425 0.58 -1.7 5,750,580 + AO356_25425 0.58 -1.0 5,750,580 + AO356_25425 0.58 -0.4 5,750,580 + AO356_25425 0.58 +0.3 5,750,581 - AO356_25425 0.58 -1.6 5,750,625 - AO356_25425 0.63 -1.9 5,750,625 - AO356_25425 0.63 -0.3 5,750,642 + AO356_25425 0.65 +1.0 5,750,683 + AO356_25425 0.70 +1.2 5,750,683 + AO356_25425 0.70 +0.7 5,750,683 + AO356_25425 0.70 -0.4 5,750,684 - AO356_25425 0.70 +0.9 5,750,871 + +2.5 5,750,872 - +1.8 5,750,907 + -0.2 5,750,907 + +0.3 5,750,907 + +0.5 5,750,907 + +1.1 5,750,908 - -0.1 5,750,908 - +1.1
Or see this region's nucleotide sequence