Experiment: Nitrate reduction with yeast extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25040 and AO356_25045 overlap by 26 nucleotides AO356_25045 and AO356_25050 overlap by 8 nucleotides AO356_25050 and AO356_25055 overlap by 4 nucleotides AO356_25055 and AO356_25060 are separated by 2 nucleotides
AO356_25040: AO356_25040 - nitrite reductase, at 5,673,848 to 5,674,417
_25040
AO356_25045: AO356_25045 - protein nirG, at 5,674,392 to 5,674,835
_25045
AO356_25050: AO356_25050 - protein nirL, at 5,674,828 to 5,675,340
_25050
AO356_25055: AO356_25055 - AsnC family transcriptional regulator, at 5,675,337 to 5,675,795
_25055
AO356_25060: AO356_25060 - protein nirF, at 5,675,798 to 5,676,976
_25060
Position (kb) 5675
5676 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5674.405 kb on + strand at 5674.406 kb on - strand at 5674.406 kb on - strand at 5674.420 kb on + strand at 5674.420 kb on + strand at 5674.420 kb on + strand at 5674.420 kb on + strand at 5674.420 kb on + strand at 5674.420 kb on + strand at 5674.421 kb on - strand at 5674.421 kb on - strand at 5674.552 kb on + strand, within AO356_25045 at 5674.553 kb on - strand, within AO356_25045 at 5674.689 kb on + strand, within AO356_25045 at 5674.689 kb on + strand, within AO356_25045 at 5674.903 kb on + strand, within AO356_25050 at 5674.904 kb on - strand, within AO356_25050 at 5674.904 kb on - strand, within AO356_25050 at 5675.006 kb on + strand, within AO356_25050 at 5675.006 kb on + strand, within AO356_25050 at 5675.006 kb on + strand, within AO356_25050 at 5675.006 kb on + strand, within AO356_25050 at 5675.007 kb on - strand, within AO356_25050 at 5675.007 kb on - strand, within AO356_25050 at 5675.007 kb on - strand, within AO356_25050 at 5675.491 kb on + strand, within AO356_25055 at 5675.491 kb on + strand, within AO356_25055 at 5675.491 kb on + strand, within AO356_25055 at 5675.509 kb on + strand, within AO356_25055 at 5675.509 kb on + strand, within AO356_25055 at 5675.510 kb on - strand, within AO356_25055 at 5675.641 kb on - strand, within AO356_25055 at 5675.723 kb on - strand, within AO356_25055 at 5676.234 kb on + strand, within AO356_25060 at 5676.234 kb on + strand, within AO356_25060 at 5676.351 kb on + strand, within AO356_25060 at 5676.351 kb on + strand, within AO356_25060 at 5676.351 kb on + strand, within AO356_25060 at 5676.351 kb on + strand, within AO356_25060 at 5676.351 kb on + strand, within AO356_25060 at 5676.351 kb on + strand, within AO356_25060 at 5676.352 kb on - strand, within AO356_25060 at 5676.352 kb on - strand, within AO356_25060 at 5676.352 kb on - strand, within AO356_25060 at 5676.352 kb on - strand, within AO356_25060 at 5676.352 kb on - strand, within AO356_25060
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction with yeast extract remove 5,674,405 + +1.1 5,674,406 - +1.0 5,674,406 - -0.1 5,674,420 + +2.1 5,674,420 + -1.0 5,674,420 + +0.5 5,674,420 + +2.0 5,674,420 + +2.1 5,674,420 + -0.9 5,674,421 - +2.2 5,674,421 - +2.2 5,674,552 + AO356_25045 0.36 -0.1 5,674,553 - AO356_25045 0.36 +2.7 5,674,689 + AO356_25045 0.67 -1.5 5,674,689 + AO356_25045 0.67 -1.2 5,674,903 + AO356_25050 0.15 +0.5 5,674,904 - AO356_25050 0.15 +0.5 5,674,904 - AO356_25050 0.15 -0.6 5,675,006 + AO356_25050 0.35 -0.3 5,675,006 + AO356_25050 0.35 +0.8 5,675,006 + AO356_25050 0.35 +3.4 5,675,006 + AO356_25050 0.35 -0.3 5,675,007 - AO356_25050 0.35 +0.6 5,675,007 - AO356_25050 0.35 -1.8 5,675,007 - AO356_25050 0.35 +0.9 5,675,491 + AO356_25055 0.34 +1.7 5,675,491 + AO356_25055 0.34 +0.4 5,675,491 + AO356_25055 0.34 +1.6 5,675,509 + AO356_25055 0.37 -1.3 5,675,509 + AO356_25055 0.37 -2.4 5,675,510 - AO356_25055 0.38 +1.4 5,675,641 - AO356_25055 0.66 +1.3 5,675,723 - AO356_25055 0.84 +2.1 5,676,234 + AO356_25060 0.37 -0.2 5,676,234 + AO356_25060 0.37 +1.5 5,676,351 + AO356_25060 0.47 -1.6 5,676,351 + AO356_25060 0.47 -1.4 5,676,351 + AO356_25060 0.47 -0.5 5,676,351 + AO356_25060 0.47 +2.4 5,676,351 + AO356_25060 0.47 +0.9 5,676,351 + AO356_25060 0.47 +1.4 5,676,352 - AO356_25060 0.47 +0.4 5,676,352 - AO356_25060 0.47 +1.6 5,676,352 - AO356_25060 0.47 +0.1 5,676,352 - AO356_25060 0.47 -1.0 5,676,352 - AO356_25060 0.47 +0.0
Or see this region's nucleotide sequence