Experiment: Nitrate reduction with yeast extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23810 and AO356_23815 are separated by 183 nucleotides AO356_23815 and AO356_23820 are separated by 245 nucleotides AO356_23820 and AO356_23825 overlap by 11 nucleotides
AO356_23810: AO356_23810 - LysR family transcriptional regulator, at 5,340,343 to 5,341,197
_23810
AO356_23815: AO356_23815 - amidase, at 5,341,381 to 5,343,087
_23815
AO356_23820: AO356_23820 - thermostable hemolysin, at 5,343,333 to 5,344,010
_23820
AO356_23825: AO356_23825 - long-chain acyl-CoA synthetase, at 5,344,000 to 5,345,481
_23825
Position (kb)
5341
5342
5343
5344 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5340.402 kb on + strand at 5340.403 kb on - strand at 5340.459 kb on + strand, within AO356_23810 at 5340.460 kb on - strand, within AO356_23810 at 5340.624 kb on + strand, within AO356_23810 at 5340.624 kb on + strand, within AO356_23810 at 5340.624 kb on + strand, within AO356_23810 at 5340.624 kb on + strand, within AO356_23810 at 5340.624 kb on + strand, within AO356_23810 at 5340.624 kb on + strand, within AO356_23810 at 5340.625 kb on - strand, within AO356_23810 at 5340.625 kb on - strand, within AO356_23810 at 5340.649 kb on + strand, within AO356_23810 at 5340.736 kb on + strand, within AO356_23810 at 5340.736 kb on + strand, within AO356_23810 at 5340.737 kb on - strand, within AO356_23810 at 5340.737 kb on - strand, within AO356_23810 at 5340.737 kb on - strand, within AO356_23810 at 5340.737 kb on - strand, within AO356_23810 at 5340.784 kb on - strand, within AO356_23810 at 5341.195 kb on + strand at 5341.196 kb on - strand at 5341.310 kb on - strand at 5341.310 kb on - strand at 5341.475 kb on + strand at 5341.475 kb on + strand at 5341.476 kb on - strand at 5341.476 kb on - strand at 5341.476 kb on - strand at 5341.811 kb on + strand, within AO356_23815 at 5341.812 kb on - strand, within AO356_23815 at 5341.812 kb on - strand, within AO356_23815 at 5341.960 kb on + strand, within AO356_23815 at 5341.961 kb on - strand, within AO356_23815 at 5342.219 kb on + strand, within AO356_23815 at 5342.219 kb on + strand, within AO356_23815 at 5342.220 kb on - strand, within AO356_23815 at 5342.220 kb on - strand, within AO356_23815 at 5342.220 kb on - strand, within AO356_23815 at 5342.643 kb on - strand, within AO356_23815 at 5342.663 kb on + strand, within AO356_23815 at 5342.663 kb on + strand, within AO356_23815 at 5342.663 kb on + strand, within AO356_23815 at 5342.664 kb on - strand, within AO356_23815 at 5342.828 kb on + strand, within AO356_23815 at 5342.829 kb on - strand, within AO356_23815 at 5342.829 kb on - strand, within AO356_23815 at 5342.847 kb on - strand, within AO356_23815 at 5342.847 kb on - strand, within AO356_23815 at 5342.978 kb on + strand at 5342.979 kb on - strand at 5342.999 kb on + strand at 5343.163 kb on - strand at 5343.163 kb on - strand at 5343.195 kb on + strand at 5343.196 kb on - strand at 5343.196 kb on - strand at 5343.238 kb on + strand at 5343.238 kb on + strand at 5343.238 kb on + strand at 5343.238 kb on + strand at 5343.238 kb on + strand at 5343.238 kb on + strand at 5343.239 kb on - strand at 5343.239 kb on - strand at 5343.252 kb on - strand at 5343.296 kb on + strand at 5343.297 kb on - strand at 5343.297 kb on - strand at 5343.310 kb on - strand at 5343.598 kb on - strand, within AO356_23820 at 5343.598 kb on - strand, within AO356_23820 at 5343.732 kb on + strand, within AO356_23820 at 5343.732 kb on + strand, within AO356_23820 at 5343.732 kb on + strand, within AO356_23820 at 5343.733 kb on - strand, within AO356_23820 at 5343.733 kb on - strand, within AO356_23820 at 5343.733 kb on - strand, within AO356_23820 at 5343.733 kb on - strand, within AO356_23820 at 5343.885 kb on + strand, within AO356_23820 at 5343.885 kb on + strand, within AO356_23820 at 5343.886 kb on - strand, within AO356_23820 at 5344.065 kb on + strand at 5344.066 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction with yeast extract remove 5,340,402 + +0.9 5,340,403 - -0.0 5,340,459 + AO356_23810 0.14 -0.6 5,340,460 - AO356_23810 0.14 -1.9 5,340,624 + AO356_23810 0.33 -0.2 5,340,624 + AO356_23810 0.33 +0.7 5,340,624 + AO356_23810 0.33 -0.2 5,340,624 + AO356_23810 0.33 +1.0 5,340,624 + AO356_23810 0.33 +0.9 5,340,624 + AO356_23810 0.33 -0.3 5,340,625 - AO356_23810 0.33 -2.0 5,340,625 - AO356_23810 0.33 -2.0 5,340,649 + AO356_23810 0.36 +2.7 5,340,736 + AO356_23810 0.46 -2.3 5,340,736 + AO356_23810 0.46 -0.2 5,340,737 - AO356_23810 0.46 +1.7 5,340,737 - AO356_23810 0.46 +0.5 5,340,737 - AO356_23810 0.46 +1.8 5,340,737 - AO356_23810 0.46 -2.7 5,340,784 - AO356_23810 0.52 +0.0 5,341,195 + -2.7 5,341,196 - -2.7 5,341,310 - -0.6 5,341,310 - -0.2 5,341,475 + +0.1 5,341,475 + -0.3 5,341,476 - +0.5 5,341,476 - +1.8 5,341,476 - +1.2 5,341,811 + AO356_23815 0.25 -1.2 5,341,812 - AO356_23815 0.25 -0.3 5,341,812 - AO356_23815 0.25 +1.0 5,341,960 + AO356_23815 0.34 +0.2 5,341,961 - AO356_23815 0.34 +1.8 5,342,219 + AO356_23815 0.49 -2.5 5,342,219 + AO356_23815 0.49 -0.8 5,342,220 - AO356_23815 0.49 +0.9 5,342,220 - AO356_23815 0.49 -2.2 5,342,220 - AO356_23815 0.49 +1.9 5,342,643 - AO356_23815 0.74 +0.6 5,342,663 + AO356_23815 0.75 +2.5 5,342,663 + AO356_23815 0.75 +0.4 5,342,663 + AO356_23815 0.75 +0.5 5,342,664 - AO356_23815 0.75 +1.1 5,342,828 + AO356_23815 0.85 +1.5 5,342,829 - AO356_23815 0.85 +1.4 5,342,829 - AO356_23815 0.85 +0.3 5,342,847 - AO356_23815 0.86 +2.2 5,342,847 - AO356_23815 0.86 -0.1 5,342,978 + +2.6 5,342,979 - +0.7 5,342,999 + -0.1 5,343,163 - +0.5 5,343,163 - +1.0 5,343,195 + +1.4 5,343,196 - +0.2 5,343,196 - +0.7 5,343,238 + -0.1 5,343,238 + -0.8 5,343,238 + +0.9 5,343,238 + +1.4 5,343,238 + +1.6 5,343,238 + +1.7 5,343,239 - +0.9 5,343,239 - -0.8 5,343,252 - +1.3 5,343,296 + -1.6 5,343,297 - -2.1 5,343,297 - +0.7 5,343,310 - +1.4 5,343,598 - AO356_23820 0.39 -2.1 5,343,598 - AO356_23820 0.39 +1.1 5,343,732 + AO356_23820 0.59 +1.4 5,343,732 + AO356_23820 0.59 +0.6 5,343,732 + AO356_23820 0.59 +0.3 5,343,733 - AO356_23820 0.59 +1.7 5,343,733 - AO356_23820 0.59 +0.5 5,343,733 - AO356_23820 0.59 -1.7 5,343,733 - AO356_23820 0.59 +0.7 5,343,885 + AO356_23820 0.81 +0.0 5,343,885 + AO356_23820 0.81 -0.9 5,343,886 - AO356_23820 0.82 -0.5 5,344,065 + -1.3 5,344,066 - +1.3
Or see this region's nucleotide sequence