Experiment: Nitrate reduction with yeast extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22755 and AO356_22760 are separated by 87 nucleotides AO356_22760 and AO356_22765 overlap by 11 nucleotides AO356_22765 and AO356_22770 are separated by 388 nucleotides AO356_22770 and AO356_22775 are separated by 202 nucleotides
AO356_22755: AO356_22755 - 6-O-methylguanine DNA methyltransferase, at 5,081,642 to 5,082,715
_22755
AO356_22760: AO356_22760 - alpha-ketoglutarate-dependent dioxygenase, at 5,082,803 to 5,083,483
_22760
AO356_22765: AO356_22765 - proline hydroxylase, at 5,083,473 to 5,084,201
_22765
AO356_22770: AO356_22770 - hypothetical protein, at 5,084,590 to 5,084,892
_22770
AO356_22775: AO356_22775 - hypothetical protein, at 5,085,095 to 5,085,445
_22775
Position (kb)
5083
5084
5085 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5082.597 kb on + strand, within AO356_22755 at 5082.597 kb on + strand, within AO356_22755 at 5082.597 kb on + strand, within AO356_22755 at 5082.597 kb on + strand, within AO356_22755 at 5082.598 kb on - strand, within AO356_22755 at 5082.598 kb on - strand, within AO356_22755 at 5082.598 kb on - strand, within AO356_22755 at 5082.622 kb on - strand at 5083.080 kb on + strand, within AO356_22760 at 5083.081 kb on - strand, within AO356_22760 at 5083.279 kb on + strand, within AO356_22760 at 5083.279 kb on + strand, within AO356_22760 at 5083.279 kb on + strand, within AO356_22760 at 5083.279 kb on + strand, within AO356_22760 at 5083.279 kb on + strand, within AO356_22760 at 5083.382 kb on + strand, within AO356_22760 at 5083.383 kb on - strand, within AO356_22760 at 5083.475 kb on - strand at 5083.475 kb on - strand at 5083.475 kb on - strand at 5083.602 kb on + strand, within AO356_22765 at 5083.602 kb on + strand, within AO356_22765 at 5083.602 kb on + strand, within AO356_22765 at 5083.603 kb on - strand, within AO356_22765 at 5083.603 kb on - strand, within AO356_22765 at 5083.753 kb on + strand, within AO356_22765 at 5083.753 kb on + strand, within AO356_22765 at 5083.777 kb on + strand, within AO356_22765 at 5083.792 kb on + strand, within AO356_22765 at 5083.793 kb on - strand, within AO356_22765 at 5083.793 kb on - strand, within AO356_22765 at 5083.931 kb on - strand, within AO356_22765 at 5083.931 kb on - strand, within AO356_22765 at 5083.931 kb on - strand, within AO356_22765 at 5083.981 kb on + strand, within AO356_22765 at 5083.982 kb on - strand, within AO356_22765 at 5083.987 kb on + strand, within AO356_22765 at 5083.988 kb on - strand, within AO356_22765 at 5084.053 kb on + strand, within AO356_22765 at 5084.053 kb on + strand, within AO356_22765 at 5084.054 kb on - strand, within AO356_22765 at 5084.054 kb on - strand, within AO356_22765 at 5084.054 kb on - strand, within AO356_22765 at 5084.055 kb on + strand, within AO356_22765 at 5084.056 kb on - strand, within AO356_22765 at 5084.056 kb on - strand, within AO356_22765 at 5084.056 kb on - strand, within AO356_22765 at 5084.161 kb on - strand at 5084.161 kb on - strand at 5084.161 kb on - strand at 5084.161 kb on - strand at 5084.393 kb on - strand at 5084.402 kb on + strand at 5084.402 kb on + strand at 5084.403 kb on - strand at 5084.530 kb on + strand at 5084.530 kb on + strand at 5084.542 kb on + strand at 5084.542 kb on + strand at 5084.542 kb on + strand at 5084.542 kb on + strand at 5084.543 kb on - strand at 5084.543 kb on - strand at 5084.543 kb on - strand at 5084.543 kb on - strand at 5084.543 kb on - strand at 5084.543 kb on - strand at 5084.759 kb on + strand, within AO356_22770 at 5084.759 kb on + strand, within AO356_22770 at 5084.760 kb on - strand, within AO356_22770 at 5084.760 kb on - strand, within AO356_22770 at 5084.760 kb on - strand, within AO356_22770 at 5084.840 kb on - strand, within AO356_22770 at 5084.974 kb on + strand at 5084.974 kb on + strand at 5084.974 kb on + strand at 5084.974 kb on + strand at 5084.975 kb on - strand at 5084.975 kb on - strand at 5084.975 kb on - strand at 5084.978 kb on - strand at 5085.008 kb on + strand at 5085.080 kb on + strand at 5085.080 kb on + strand at 5085.081 kb on - strand at 5085.094 kb on + strand at 5085.094 kb on + strand at 5085.094 kb on + strand at 5085.094 kb on + strand at 5085.094 kb on + strand at 5085.094 kb on + strand at 5085.094 kb on + strand at 5085.094 kb on + strand at 5085.094 kb on + strand at 5085.094 kb on + strand at 5085.095 kb on - strand at 5085.095 kb on - strand at 5085.095 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction with yeast extract remove 5,082,597 + AO356_22755 0.89 +0.0 5,082,597 + AO356_22755 0.89 -0.5 5,082,597 + AO356_22755 0.89 -0.0 5,082,597 + AO356_22755 0.89 -0.6 5,082,598 - AO356_22755 0.89 +0.3 5,082,598 - AO356_22755 0.89 -0.3 5,082,598 - AO356_22755 0.89 +0.8 5,082,622 - +1.6 5,083,080 + AO356_22760 0.41 -1.0 5,083,081 - AO356_22760 0.41 -1.4 5,083,279 + AO356_22760 0.70 -1.7 5,083,279 + AO356_22760 0.70 -0.4 5,083,279 + AO356_22760 0.70 +1.0 5,083,279 + AO356_22760 0.70 +0.2 5,083,279 + AO356_22760 0.70 -0.2 5,083,382 + AO356_22760 0.85 +0.1 5,083,383 - AO356_22760 0.85 -2.6 5,083,475 - +1.7 5,083,475 - -0.5 5,083,475 - -0.6 5,083,602 + AO356_22765 0.18 +0.4 5,083,602 + AO356_22765 0.18 +0.0 5,083,602 + AO356_22765 0.18 +0.6 5,083,603 - AO356_22765 0.18 +0.1 5,083,603 - AO356_22765 0.18 -0.8 5,083,753 + AO356_22765 0.38 -1.4 5,083,753 + AO356_22765 0.38 -0.3 5,083,777 + AO356_22765 0.42 -0.0 5,083,792 + AO356_22765 0.44 -0.6 5,083,793 - AO356_22765 0.44 -0.6 5,083,793 - AO356_22765 0.44 -1.8 5,083,931 - AO356_22765 0.63 +1.1 5,083,931 - AO356_22765 0.63 +1.5 5,083,931 - AO356_22765 0.63 -1.6 5,083,981 + AO356_22765 0.70 +0.8 5,083,982 - AO356_22765 0.70 -1.9 5,083,987 + AO356_22765 0.71 -0.1 5,083,988 - AO356_22765 0.71 -0.6 5,084,053 + AO356_22765 0.80 -1.2 5,084,053 + AO356_22765 0.80 -2.1 5,084,054 - AO356_22765 0.80 +1.7 5,084,054 - AO356_22765 0.80 -0.7 5,084,054 - AO356_22765 0.80 +1.0 5,084,055 + AO356_22765 0.80 -0.3 5,084,056 - AO356_22765 0.80 -1.1 5,084,056 - AO356_22765 0.80 +1.6 5,084,056 - AO356_22765 0.80 +1.3 5,084,161 - +3.7 5,084,161 - -0.3 5,084,161 - +0.9 5,084,161 - +1.2 5,084,393 - -0.2 5,084,402 + -0.5 5,084,402 + -0.9 5,084,403 - -2.4 5,084,530 + -0.2 5,084,530 + +0.3 5,084,542 + +0.5 5,084,542 + -1.8 5,084,542 + +0.8 5,084,542 + -1.5 5,084,543 - -1.1 5,084,543 - +1.9 5,084,543 - -0.8 5,084,543 - -2.7 5,084,543 - +0.7 5,084,543 - +0.9 5,084,759 + AO356_22770 0.56 -1.3 5,084,759 + AO356_22770 0.56 -1.6 5,084,760 - AO356_22770 0.56 -1.5 5,084,760 - AO356_22770 0.56 +2.9 5,084,760 - AO356_22770 0.56 +1.1 5,084,840 - AO356_22770 0.83 +0.8 5,084,974 + -1.0 5,084,974 + -1.1 5,084,974 + +0.8 5,084,974 + +1.1 5,084,975 - +1.2 5,084,975 - +1.1 5,084,975 - -1.7 5,084,978 - +1.3 5,085,008 + +0.9 5,085,080 + -0.5 5,085,080 + +0.6 5,085,081 - -0.8 5,085,094 + -0.8 5,085,094 + -1.5 5,085,094 + -0.1 5,085,094 + +2.0 5,085,094 + +1.6 5,085,094 + -1.1 5,085,094 + +0.2 5,085,094 + +1.8 5,085,094 + -2.0 5,085,094 + -1.0 5,085,095 - -0.5 5,085,095 - -0.6 5,085,095 - -0.2
Or see this region's nucleotide sequence