Experiment: Nitrate reduction with yeast extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22490 and AO356_22495 are separated by 181 nucleotides AO356_22495 and AO356_22500 overlap by 4 nucleotides AO356_22500 and AO356_22505 are separated by 125 nucleotides
AO356_22490: AO356_22490 - phosphohydrolase, at 5,026,140 to 5,027,579
_22490
AO356_22495: AO356_22495 - RNA polymerase subunit sigma-24, at 5,027,761 to 5,028,267
_22495
AO356_22500: AO356_22500 - hypothetical protein, at 5,028,264 to 5,029,190
_22500
AO356_22505: AO356_22505 - TonB-dependent receptor, at 5,029,316 to 5,031,715
_22505
Position (kb) 5028
5029
5030 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5027.389 kb on + strand, within AO356_22490 at 5027.506 kb on + strand at 5027.506 kb on + strand at 5027.506 kb on + strand at 5027.506 kb on + strand at 5028.017 kb on + strand, within AO356_22495 at 5028.017 kb on + strand, within AO356_22495 at 5028.017 kb on + strand, within AO356_22495 at 5028.017 kb on + strand, within AO356_22495 at 5028.017 kb on + strand, within AO356_22495 at 5028.018 kb on - strand, within AO356_22495 at 5028.018 kb on - strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.214 kb on + strand, within AO356_22495 at 5028.215 kb on - strand, within AO356_22495 at 5028.215 kb on - strand, within AO356_22495 at 5028.215 kb on - strand, within AO356_22495 at 5028.215 kb on - strand, within AO356_22495 at 5028.215 kb on - strand, within AO356_22495 at 5028.215 kb on - strand, within AO356_22495 at 5028.215 kb on - strand, within AO356_22495 at 5028.433 kb on + strand, within AO356_22500 at 5028.433 kb on + strand, within AO356_22500 at 5028.434 kb on - strand, within AO356_22500 at 5028.434 kb on - strand, within AO356_22500 at 5028.561 kb on + strand, within AO356_22500 at 5028.561 kb on + strand, within AO356_22500 at 5028.561 kb on + strand, within AO356_22500 at 5028.561 kb on + strand, within AO356_22500 at 5028.561 kb on + strand, within AO356_22500 at 5028.561 kb on + strand, within AO356_22500 at 5028.562 kb on - strand, within AO356_22500 at 5028.562 kb on - strand, within AO356_22500 at 5029.224 kb on - strand at 5029.224 kb on - strand at 5029.466 kb on + strand at 5029.466 kb on + strand at 5029.466 kb on + strand at 5029.521 kb on - strand at 5029.778 kb on + strand, within AO356_22505 at 5029.779 kb on - strand, within AO356_22505 at 5029.779 kb on - strand, within AO356_22505 at 5029.779 kb on - strand, within AO356_22505 at 5029.779 kb on - strand, within AO356_22505 at 5030.117 kb on + strand, within AO356_22505 at 5030.117 kb on + strand, within AO356_22505 at 5030.118 kb on - strand, within AO356_22505
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction with yeast extract remove 5,027,389 + AO356_22490 0.87 +0.2 5,027,506 + +1.8 5,027,506 + -1.4 5,027,506 + +0.9 5,027,506 + +0.6 5,028,017 + AO356_22495 0.50 -1.7 5,028,017 + AO356_22495 0.50 +1.9 5,028,017 + AO356_22495 0.50 +1.3 5,028,017 + AO356_22495 0.50 -0.8 5,028,017 + AO356_22495 0.50 +0.7 5,028,018 - AO356_22495 0.51 -1.8 5,028,018 - AO356_22495 0.51 +0.8 5,028,214 + AO356_22495 0.89 +2.0 5,028,214 + AO356_22495 0.89 +1.5 5,028,214 + +1.6 5,028,214 + AO356_22495 0.89 -2.2 5,028,214 + AO356_22495 0.89 +1.0 5,028,214 + AO356_22495 0.89 +1.1 5,028,214 + AO356_22495 0.89 -0.5 5,028,214 + AO356_22495 0.89 +1.7 5,028,214 + AO356_22495 0.89 +0.6 5,028,214 + AO356_22495 0.89 +0.0 5,028,215 - AO356_22495 0.90 -2.0 5,028,215 - AO356_22495 0.90 +1.0 5,028,215 - AO356_22495 0.90 +0.3 5,028,215 - AO356_22495 0.90 +0.2 5,028,215 - AO356_22495 0.90 -2.7 5,028,215 - AO356_22495 0.90 -1.1 5,028,215 - AO356_22495 0.90 -0.4 5,028,433 + AO356_22500 0.18 +0.0 5,028,433 + AO356_22500 0.18 +2.7 5,028,434 - AO356_22500 0.18 -0.6 5,028,434 - AO356_22500 0.18 +0.2 5,028,561 + AO356_22500 0.32 -0.8 5,028,561 + AO356_22500 0.32 -2.1 5,028,561 + AO356_22500 0.32 -0.0 5,028,561 + AO356_22500 0.32 -1.7 5,028,561 + AO356_22500 0.32 +3.2 5,028,561 + AO356_22500 0.32 +0.6 5,028,562 - AO356_22500 0.32 -0.4 5,028,562 - AO356_22500 0.32 +2.7 5,029,224 - +1.7 5,029,224 - -1.9 5,029,466 + -0.8 5,029,466 + +0.3 5,029,466 + +2.2 5,029,521 - +1.7 5,029,778 + AO356_22505 0.19 -1.6 5,029,779 - AO356_22505 0.19 +1.6 5,029,779 - AO356_22505 0.19 +1.0 5,029,779 - AO356_22505 0.19 -0.6 5,029,779 - AO356_22505 0.19 +1.2 5,030,117 + AO356_22505 0.33 +0.2 5,030,117 + AO356_22505 0.33 +0.2 5,030,118 - AO356_22505 0.33 -0.2
Or see this region's nucleotide sequence