Experiment: Nitrate reduction with yeast extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22345 and AO356_22350 are separated by 1 nucleotides AO356_22350 and AO356_22355 are separated by 176 nucleotides AO356_22355 and AO356_22360 overlap by 4 nucleotides
AO356_22345: AO356_22345 - potassium transporter KefC, at 4,995,646 to 4,997,472
_22345
AO356_22350: AO356_22350 - potassium transporter KefF, at 4,997,474 to 4,997,995
_22350
AO356_22355: AO356_22355 - histidine kinase, at 4,998,172 to 4,999,518
_22355
AO356_22360: AO356_22360 - chemotaxis protein CheY, at 4,999,515 to 5,000,225
_22360
Position (kb)
4998
4999
5000 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4997.324 kb on + strand at 4997.368 kb on + strand at 4997.369 kb on - strand at 4997.369 kb on - strand at 4997.373 kb on - strand at 4997.438 kb on + strand at 4997.438 kb on + strand at 4997.438 kb on + strand at 4997.438 kb on + strand at 4997.508 kb on + strand at 4997.508 kb on + strand at 4997.508 kb on + strand at 4997.509 kb on - strand at 4997.509 kb on - strand at 4997.509 kb on - strand at 4997.550 kb on + strand, within AO356_22350 at 4997.595 kb on + strand, within AO356_22350 at 4997.595 kb on + strand, within AO356_22350 at 4997.595 kb on + strand, within AO356_22350 at 4997.595 kb on + strand, within AO356_22350 at 4997.596 kb on - strand, within AO356_22350 at 4997.596 kb on - strand, within AO356_22350 at 4997.596 kb on - strand, within AO356_22350 at 4997.596 kb on - strand, within AO356_22350 at 4997.596 kb on - strand, within AO356_22350 at 4997.596 kb on - strand, within AO356_22350 at 4997.733 kb on + strand, within AO356_22350 at 4997.793 kb on + strand, within AO356_22350 at 4997.793 kb on + strand, within AO356_22350 at 4997.799 kb on + strand, within AO356_22350 at 4997.799 kb on + strand, within AO356_22350 at 4997.799 kb on + strand, within AO356_22350 at 4997.799 kb on + strand, within AO356_22350 at 4997.800 kb on - strand, within AO356_22350 at 4997.889 kb on + strand, within AO356_22350 at 4997.890 kb on - strand, within AO356_22350 at 4997.890 kb on - strand, within AO356_22350 at 4997.900 kb on + strand, within AO356_22350 at 4997.900 kb on + strand, within AO356_22350 at 4997.900 kb on + strand, within AO356_22350 at 4997.900 kb on + strand, within AO356_22350 at 4997.901 kb on - strand, within AO356_22350 at 4997.901 kb on - strand, within AO356_22350 at 4997.901 kb on - strand, within AO356_22350 at 4997.901 kb on - strand, within AO356_22350 at 4997.901 kb on - strand, within AO356_22350 at 4997.979 kb on + strand at 4997.987 kb on - strand at 4998.069 kb on + strand at 4998.070 kb on - strand at 4998.353 kb on + strand, within AO356_22355 at 4998.443 kb on + strand, within AO356_22355 at 4998.506 kb on + strand, within AO356_22355 at 4998.506 kb on + strand, within AO356_22355 at 4998.506 kb on + strand, within AO356_22355 at 4998.506 kb on + strand, within AO356_22355 at 4998.506 kb on + strand, within AO356_22355 at 4998.507 kb on - strand, within AO356_22355 at 4998.507 kb on - strand, within AO356_22355 at 4998.507 kb on - strand, within AO356_22355 at 4998.507 kb on - strand, within AO356_22355 at 4999.139 kb on + strand, within AO356_22355 at 4999.139 kb on + strand, within AO356_22355 at 4999.140 kb on - strand, within AO356_22355 at 4999.546 kb on + strand at 4999.728 kb on + strand, within AO356_22360 at 5000.147 kb on + strand, within AO356_22360 at 5000.148 kb on - strand, within AO356_22360 at 5000.148 kb on - strand, within AO356_22360 at 5000.148 kb on - strand, within AO356_22360 at 5000.149 kb on + strand, within AO356_22360 at 5000.149 kb on + strand, within AO356_22360 at 5000.150 kb on - strand, within AO356_22360 at 5000.150 kb on - strand, within AO356_22360 at 5000.211 kb on + strand at 5000.214 kb on + strand at 5000.214 kb on + strand at 5000.215 kb on - strand at 5000.215 kb on - strand at 5000.215 kb on - strand at 5000.248 kb on + strand at 5000.248 kb on + strand at 5000.249 kb on - strand at 5000.261 kb on - strand at 5000.261 kb on - strand at 5000.263 kb on + strand at 5000.263 kb on + strand at 5000.263 kb on + strand at 5000.263 kb on + strand at 5000.263 kb on + strand at 5000.264 kb on - strand at 5000.290 kb on + strand at 5000.290 kb on + strand at 5000.290 kb on + strand at 5000.291 kb on - strand at 5000.291 kb on - strand at 5000.291 kb on - strand at 5000.291 kb on - strand at 5000.291 kb on - strand at 5000.291 kb on - strand at 5000.307 kb on - strand at 5000.325 kb on + strand at 5000.326 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction with yeast extract remove 4,997,324 + -2.1 4,997,368 + -1.7 4,997,369 - +1.1 4,997,369 - -0.0 4,997,373 - +2.3 4,997,438 + -1.7 4,997,438 + -0.5 4,997,438 + +1.3 4,997,438 + -0.4 4,997,508 + -1.1 4,997,508 + +0.2 4,997,508 + +0.5 4,997,509 - +1.5 4,997,509 - -0.0 4,997,509 - +1.6 4,997,550 + AO356_22350 0.15 -0.6 4,997,595 + AO356_22350 0.23 +0.5 4,997,595 + AO356_22350 0.23 -2.0 4,997,595 + AO356_22350 0.23 -0.9 4,997,595 + AO356_22350 0.23 -0.3 4,997,596 - AO356_22350 0.23 +1.4 4,997,596 - AO356_22350 0.23 -0.4 4,997,596 - AO356_22350 0.23 +0.8 4,997,596 - AO356_22350 0.23 -2.3 4,997,596 - AO356_22350 0.23 +1.5 4,997,596 - AO356_22350 0.23 -1.9 4,997,733 + AO356_22350 0.50 -0.9 4,997,793 + AO356_22350 0.61 +0.7 4,997,793 + AO356_22350 0.61 -0.7 4,997,799 + AO356_22350 0.62 -3.2 4,997,799 + AO356_22350 0.62 -0.8 4,997,799 + AO356_22350 0.62 +0.5 4,997,799 + AO356_22350 0.62 +0.6 4,997,800 - AO356_22350 0.62 -1.5 4,997,889 + AO356_22350 0.80 -0.4 4,997,890 - AO356_22350 0.80 -1.7 4,997,890 - AO356_22350 0.80 -0.9 4,997,900 + AO356_22350 0.82 +2.5 4,997,900 + AO356_22350 0.82 +1.1 4,997,900 + AO356_22350 0.82 +0.9 4,997,900 + AO356_22350 0.82 +1.4 4,997,901 - AO356_22350 0.82 +1.5 4,997,901 - AO356_22350 0.82 +0.9 4,997,901 - AO356_22350 0.82 +1.3 4,997,901 - AO356_22350 0.82 -2.0 4,997,901 - AO356_22350 0.82 -0.0 4,997,979 + +1.6 4,997,987 - +0.4 4,998,069 + -1.4 4,998,070 - +0.3 4,998,353 + AO356_22355 0.13 -1.5 4,998,443 + AO356_22355 0.20 -2.4 4,998,506 + AO356_22355 0.25 +0.2 4,998,506 + AO356_22355 0.25 -1.4 4,998,506 + AO356_22355 0.25 +1.6 4,998,506 + AO356_22355 0.25 -0.3 4,998,506 + AO356_22355 0.25 +0.5 4,998,507 - AO356_22355 0.25 -0.2 4,998,507 - AO356_22355 0.25 -1.6 4,998,507 - AO356_22355 0.25 -0.2 4,998,507 - AO356_22355 0.25 -0.2 4,999,139 + AO356_22355 0.72 +0.4 4,999,139 + AO356_22355 0.72 +0.6 4,999,140 - AO356_22355 0.72 +1.8 4,999,546 + -3.0 4,999,728 + AO356_22360 0.30 -1.1 5,000,147 + AO356_22360 0.89 -0.7 5,000,148 - AO356_22360 0.89 +0.8 5,000,148 - AO356_22360 0.89 +1.2 5,000,148 - AO356_22360 0.89 +0.1 5,000,149 + AO356_22360 0.89 +1.3 5,000,149 + AO356_22360 0.89 +0.4 5,000,150 - AO356_22360 0.89 -2.3 5,000,150 - AO356_22360 0.89 +0.5 5,000,211 + +1.3 5,000,214 + +0.3 5,000,214 + +0.6 5,000,215 - +2.1 5,000,215 - -0.1 5,000,215 - -0.9 5,000,248 + +1.3 5,000,248 + +0.7 5,000,249 - -2.6 5,000,261 - +1.1 5,000,261 - -2.3 5,000,263 + -1.2 5,000,263 + +1.0 5,000,263 + +0.9 5,000,263 + +0.6 5,000,263 + +0.9 5,000,264 - +1.6 5,000,290 + +0.4 5,000,290 + -0.5 5,000,290 + +0.0 5,000,291 - -2.3 5,000,291 - +0.5 5,000,291 - -2.3 5,000,291 - -2.0 5,000,291 - +0.3 5,000,291 - +1.1 5,000,307 - +1.7 5,000,325 + -0.2 5,000,326 - -1.3
Or see this region's nucleotide sequence