Experiment: Nitrate reduction with yeast extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_10185 and AO356_10190 are separated by 249 nucleotides AO356_10190 and AO356_10195 are separated by 20 nucleotides AO356_10195 and AO356_10200 overlap by 4 nucleotides
AO356_10185: AO356_10185 - glutamate--cysteine ligase, at 2,297,998 to 2,299,581
_10185
AO356_10190: AO356_10190 - taurine ABC transporter substrate-binding protein, at 2,299,831 to 2,300,808
_10190
AO356_10195: AO356_10195 - taurine transporter ATP-binding subunit, at 2,300,829 to 2,301,623
_10195
AO356_10200: AO356_10200 - taurine ABC transporter permease, at 2,301,620 to 2,302,459
_10200
Position (kb)
2299
2300
2301 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2298.868 kb on - strand, within AO356_10185 at 2298.895 kb on + strand, within AO356_10185 at 2298.895 kb on + strand, within AO356_10185 at 2298.895 kb on + strand, within AO356_10185 at 2298.896 kb on - strand, within AO356_10185 at 2298.899 kb on - strand, within AO356_10185 at 2298.928 kb on + strand, within AO356_10185 at 2298.928 kb on + strand, within AO356_10185 at 2298.929 kb on - strand, within AO356_10185 at 2298.976 kb on + strand, within AO356_10185 at 2298.977 kb on - strand, within AO356_10185 at 2299.066 kb on + strand, within AO356_10185 at 2299.066 kb on + strand, within AO356_10185 at 2299.066 kb on + strand, within AO356_10185 at 2299.067 kb on - strand, within AO356_10185 at 2299.067 kb on - strand, within AO356_10185 at 2299.575 kb on + strand at 2299.575 kb on + strand at 2299.576 kb on - strand at 2299.576 kb on - strand at 2299.576 kb on - strand at 2299.783 kb on - strand at 2299.970 kb on - strand, within AO356_10190 at 2300.184 kb on + strand, within AO356_10190 at 2300.184 kb on + strand, within AO356_10190 at 2300.185 kb on - strand, within AO356_10190 at 2300.245 kb on + strand, within AO356_10190 at 2300.246 kb on - strand, within AO356_10190 at 2300.362 kb on + strand, within AO356_10190 at 2300.362 kb on + strand, within AO356_10190 at 2300.362 kb on + strand, within AO356_10190 at 2300.362 kb on + strand, within AO356_10190 at 2300.362 kb on + strand, within AO356_10190 at 2300.363 kb on - strand, within AO356_10190 at 2300.363 kb on - strand, within AO356_10190 at 2300.363 kb on - strand, within AO356_10190 at 2300.363 kb on - strand, within AO356_10190 at 2300.363 kb on - strand, within AO356_10190 at 2300.363 kb on - strand, within AO356_10190 at 2300.536 kb on + strand, within AO356_10190 at 2300.628 kb on + strand, within AO356_10190 at 2300.639 kb on + strand, within AO356_10190 at 2300.640 kb on - strand, within AO356_10190 at 2300.770 kb on + strand at 2300.771 kb on - strand at 2300.779 kb on + strand at 2300.779 kb on + strand at 2300.780 kb on - strand at 2300.780 kb on - strand at 2300.794 kb on + strand at 2300.794 kb on + strand at 2300.794 kb on + strand at 2300.794 kb on + strand at 2300.794 kb on + strand at 2300.794 kb on + strand at 2300.794 kb on + strand at 2300.794 kb on + strand at 2300.794 kb on + strand at 2300.794 kb on + strand at 2300.795 kb on - strand at 2300.795 kb on - strand at 2300.795 kb on - strand at 2300.795 kb on - strand at 2300.795 kb on - strand at 2300.795 kb on - strand at 2300.839 kb on + strand at 2300.865 kb on + strand at 2300.897 kb on + strand at 2300.897 kb on + strand at 2300.898 kb on - strand at 2300.898 kb on - strand at 2300.898 kb on - strand at 2301.629 kb on + strand at 2301.629 kb on + strand at 2301.630 kb on - strand at 2301.701 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction with yeast extract remove 2,298,868 - AO356_10185 0.55 +1.0 2,298,895 + AO356_10185 0.57 -0.4 2,298,895 + AO356_10185 0.57 -0.1 2,298,895 + AO356_10185 0.57 -0.6 2,298,896 - AO356_10185 0.57 +0.2 2,298,899 - AO356_10185 0.57 -1.0 2,298,928 + AO356_10185 0.59 -0.3 2,298,928 + AO356_10185 0.59 -1.3 2,298,929 - AO356_10185 0.59 +1.6 2,298,976 + AO356_10185 0.62 +0.1 2,298,977 - AO356_10185 0.62 -1.0 2,299,066 + AO356_10185 0.67 +0.3 2,299,066 + AO356_10185 0.67 +0.4 2,299,066 + AO356_10185 0.67 +0.2 2,299,067 - AO356_10185 0.67 -0.5 2,299,067 - AO356_10185 0.67 +0.8 2,299,575 + -1.5 2,299,575 + -0.9 2,299,576 - -2.3 2,299,576 - +0.3 2,299,576 - -1.0 2,299,783 - -0.3 2,299,970 - AO356_10190 0.14 +2.4 2,300,184 + AO356_10190 0.36 +2.8 2,300,184 + AO356_10190 0.36 +0.1 2,300,185 - AO356_10190 0.36 +0.7 2,300,245 + AO356_10190 0.42 +1.2 2,300,246 - AO356_10190 0.42 -0.7 2,300,362 + AO356_10190 0.54 +2.1 2,300,362 + AO356_10190 0.54 -1.7 2,300,362 + AO356_10190 0.54 +2.7 2,300,362 + AO356_10190 0.54 +0.4 2,300,362 + AO356_10190 0.54 +2.1 2,300,363 - AO356_10190 0.54 -2.6 2,300,363 - AO356_10190 0.54 -1.0 2,300,363 - AO356_10190 0.54 -0.8 2,300,363 - AO356_10190 0.54 -0.5 2,300,363 - AO356_10190 0.54 +0.3 2,300,363 - AO356_10190 0.54 -0.2 2,300,536 + AO356_10190 0.72 +0.4 2,300,628 + AO356_10190 0.81 +2.1 2,300,639 + AO356_10190 0.83 -1.4 2,300,640 - AO356_10190 0.83 -0.6 2,300,770 + +1.5 2,300,771 - -0.4 2,300,779 + -1.6 2,300,779 + +1.1 2,300,780 - +0.9 2,300,780 - -2.1 2,300,794 + +1.7 2,300,794 + -2.8 2,300,794 + -1.7 2,300,794 + +1.3 2,300,794 + -2.3 2,300,794 + +1.7 2,300,794 + -1.3 2,300,794 + -2.4 2,300,794 + +1.0 2,300,794 + -0.3 2,300,795 - -2.5 2,300,795 - -2.2 2,300,795 - +0.2 2,300,795 - -1.9 2,300,795 - -1.7 2,300,795 - +0.4 2,300,839 + -0.3 2,300,865 + -1.1 2,300,897 + +0.6 2,300,897 + +1.0 2,300,898 - +1.3 2,300,898 - +0.7 2,300,898 - -2.7 2,301,629 + +1.4 2,301,629 + +1.6 2,301,630 - +2.2 2,301,701 - -0.5
Or see this region's nucleotide sequence