Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_09230

Experiment: Nitrate reduction with yeast extract

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_09225 and AO356_09230 are separated by 42 nucleotidesAO356_09230 and AO356_09235 are separated by 804 nucleotides AO356_09225: AO356_09225 - 3-oxoacyl-ACP synthase, at 2,084,312 to 2,085,538 _09225 AO356_09230: AO356_09230 - excinuclease, at 2,085,581 to 2,086,027 _09230 AO356_09235: AO356_09235 - hypothetical protein, at 2,086,832 to 2,087,365 _09235 Position (kb) 2085 2086 2087Strain fitness (log2 ratio) -1 0 1 2at 2084.714 kb on + strand, within AO356_09225at 2084.715 kb on - strand, within AO356_09225at 2084.715 kb on - strand, within AO356_09225at 2084.715 kb on - strand, within AO356_09225at 2084.715 kb on - strand, within AO356_09225at 2084.834 kb on + strand, within AO356_09225at 2084.834 kb on + strand, within AO356_09225at 2084.834 kb on + strand, within AO356_09225at 2084.835 kb on - strand, within AO356_09225at 2084.927 kb on + strand, within AO356_09225at 2085.201 kb on - strand, within AO356_09225at 2085.380 kb on + strand, within AO356_09225at 2085.381 kb on - strand, within AO356_09225at 2085.434 kb on + strandat 2085.435 kb on - strandat 2085.470 kb on + strandat 2085.564 kb on - strandat 2085.926 kb on + strand, within AO356_09230at 2085.926 kb on + strand, within AO356_09230at 2085.926 kb on + strand, within AO356_09230at 2085.927 kb on - strand, within AO356_09230at 2085.959 kb on + strand, within AO356_09230at 2085.959 kb on + strand, within AO356_09230at 2085.959 kb on + strand, within AO356_09230at 2085.960 kb on - strand, within AO356_09230at 2085.960 kb on - strand, within AO356_09230at 2085.960 kb on - strand, within AO356_09230at 2086.047 kb on + strandat 2086.048 kb on - strandat 2086.408 kb on + strandat 2086.455 kb on + strandat 2086.523 kb on + strandat 2086.656 kb on + strandat 2086.721 kb on + strandat 2086.721 kb on + strandat 2086.961 kb on - strand, within AO356_09235at 2086.961 kb on - strand, within AO356_09235at 2087.015 kb on - strand, within AO356_09235at 2087.015 kb on - strand, within AO356_09235at 2087.015 kb on - strand, within AO356_09235at 2087.015 kb on - strand, within AO356_09235at 2087.015 kb on - strand, within AO356_09235

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction with yeast extract
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2,084,714 + AO356_09225 0.33 -1.3
2,084,715 - AO356_09225 0.33 -0.7
2,084,715 - AO356_09225 0.33 +1.9
2,084,715 - AO356_09225 0.33 -1.6
2,084,715 - AO356_09225 0.33 -1.5
2,084,834 + AO356_09225 0.43 +0.8
2,084,834 + AO356_09225 0.43 +0.1
2,084,834 + AO356_09225 0.43 +0.6
2,084,835 - AO356_09225 0.43 -0.5
2,084,927 + AO356_09225 0.50 -1.5
2,085,201 - AO356_09225 0.72 -1.7
2,085,380 + AO356_09225 0.87 -0.3
2,085,381 - AO356_09225 0.87 -1.6
2,085,434 + +1.2
2,085,435 - -0.6
2,085,470 + +0.1
2,085,564 - -0.6
2,085,926 + AO356_09230 0.77 +0.1
2,085,926 + AO356_09230 0.77 -0.1
2,085,926 + AO356_09230 0.77 +0.0
2,085,927 - AO356_09230 0.77 +1.1
2,085,959 + AO356_09230 0.85 +1.3
2,085,959 + AO356_09230 0.85 -1.6
2,085,959 + AO356_09230 0.85 -0.4
2,085,960 - AO356_09230 0.85 +1.6
2,085,960 - AO356_09230 0.85 -0.3
2,085,960 - AO356_09230 0.85 +1.2
2,086,047 + +0.1
2,086,048 - -0.3
2,086,408 + -0.3
2,086,455 + +2.6
2,086,523 + +0.7
2,086,656 + -0.6
2,086,721 + +1.3
2,086,721 + -0.8
2,086,961 - AO356_09235 0.24 +0.9
2,086,961 - AO356_09235 0.24 +0.3
2,087,015 - AO356_09235 0.34 +0.1
2,087,015 - AO356_09235 0.34 -1.6
2,087,015 - AO356_09235 0.34 +0.2
2,087,015 - AO356_09235 0.34 -1.4
2,087,015 - AO356_09235 0.34 -0.2

Or see this region's nucleotide sequence