Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_619

Experiment: Magnesium chloride 0.125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_618 and Pf6N2E2_619 overlap by 4 nucleotidesPf6N2E2_619 and Pf6N2E2_620 are separated by 337 nucleotides Pf6N2E2_618 - Exonuclease SbcC, at 700,409 to 704,050 _618 Pf6N2E2_619 - Exonuclease SbcD, at 704,047 to 705,291 _619 Pf6N2E2_620 - BatD, at 705,629 to 707,266 _620 Position (kb) 704 705 706Strain fitness (log2 ratio) -2 -1 0 1at 703.244 kb on + strand, within Pf6N2E2_618at 703.461 kb on + strand, within Pf6N2E2_618at 703.462 kb on - strand, within Pf6N2E2_618at 703.611 kb on + strand, within Pf6N2E2_618at 704.130 kb on - strandat 704.234 kb on + strand, within Pf6N2E2_619at 704.624 kb on + strand, within Pf6N2E2_619at 704.624 kb on + strand, within Pf6N2E2_619at 704.625 kb on - strand, within Pf6N2E2_619at 704.770 kb on - strand, within Pf6N2E2_619at 705.102 kb on - strand, within Pf6N2E2_619at 705.147 kb on + strand, within Pf6N2E2_619at 705.296 kb on + strandat 705.296 kb on + strandat 705.579 kb on + strandat 705.630 kb on + strandat 705.631 kb on - strandat 705.754 kb on - strandat 705.892 kb on + strand, within Pf6N2E2_620at 705.893 kb on - strand, within Pf6N2E2_620at 705.893 kb on - strand, within Pf6N2E2_620at 706.036 kb on - strand, within Pf6N2E2_620

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Per-strain Table

Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M
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703,244 + Pf6N2E2_618 0.78 +0.4
703,461 + Pf6N2E2_618 0.84 +0.7
703,462 - Pf6N2E2_618 0.84 -0.5
703,611 + Pf6N2E2_618 0.88 +1.4
704,130 - -0.3
704,234 + Pf6N2E2_619 0.15 -1.8
704,624 + Pf6N2E2_619 0.46 +0.6
704,624 + Pf6N2E2_619 0.46 -0.6
704,625 - Pf6N2E2_619 0.46 +0.5
704,770 - Pf6N2E2_619 0.58 +0.4
705,102 - Pf6N2E2_619 0.85 -2.4
705,147 + Pf6N2E2_619 0.88 -1.8
705,296 + -0.1
705,296 + +0.1
705,579 + +0.8
705,630 + -2.3
705,631 - -1.9
705,754 - -0.5
705,892 + Pf6N2E2_620 0.16 -0.5
705,893 - Pf6N2E2_620 0.16 -0.1
705,893 - Pf6N2E2_620 0.16 +1.7
706,036 - Pf6N2E2_620 0.25 -1.2

Or see this region's nucleotide sequence