Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5537 and Pf6N2E2_5538 are separated by 127 nucleotides Pf6N2E2_5538 and Pf6N2E2_5539 overlap by 11 nucleotides Pf6N2E2_5539 and Pf6N2E2_5540 are separated by 14 nucleotides
Pf6N2E2_5537 - Holliday junction DNA helicase RuvB, at 6,353,119 to 6,354,180
_5537
Pf6N2E2_5538 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 6,354,308 to 6,354,775
_5538
Pf6N2E2_5539 - MotA/TolQ/ExbB proton channel family protein, at 6,354,765 to 6,355,460
_5539
Pf6N2E2_5540 - Tol biopolymer transport system, TolR protein, at 6,355,475 to 6,355,936
_5540
Position (kb)
6354
6355 Strain fitness (log2 ratio)
-2
-1
0
1 at 6354.052 kb on + strand, within Pf6N2E2_5537 at 6354.113 kb on + strand at 6354.114 kb on - strand at 6354.149 kb on + strand at 6354.295 kb on + strand at 6354.295 kb on + strand at 6354.302 kb on + strand at 6354.302 kb on + strand at 6354.307 kb on + strand at 6354.307 kb on + strand at 6354.307 kb on + strand at 6354.356 kb on + strand, within Pf6N2E2_5538 at 6354.356 kb on + strand, within Pf6N2E2_5538 at 6354.359 kb on + strand, within Pf6N2E2_5538 at 6354.359 kb on + strand, within Pf6N2E2_5538 at 6354.389 kb on + strand, within Pf6N2E2_5538 at 6354.389 kb on + strand, within Pf6N2E2_5538 at 6354.389 kb on + strand, within Pf6N2E2_5538 at 6354.392 kb on + strand, within Pf6N2E2_5538 at 6354.392 kb on + strand, within Pf6N2E2_5538 at 6354.392 kb on + strand, within Pf6N2E2_5538 at 6354.392 kb on + strand, within Pf6N2E2_5538 at 6354.401 kb on + strand, within Pf6N2E2_5538 at 6354.401 kb on + strand, within Pf6N2E2_5538 at 6354.412 kb on + strand, within Pf6N2E2_5538 at 6354.552 kb on + strand, within Pf6N2E2_5538 at 6354.552 kb on + strand, within Pf6N2E2_5538 at 6354.673 kb on + strand, within Pf6N2E2_5538 at 6354.673 kb on + strand, within Pf6N2E2_5538 at 6354.705 kb on + strand, within Pf6N2E2_5538 at 6354.705 kb on + strand, within Pf6N2E2_5538 at 6354.739 kb on + strand at 6355.436 kb on + strand at 6355.436 kb on + strand at 6355.458 kb on + strand at 6355.458 kb on + strand at 6355.458 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 6,354,052 + Pf6N2E2_5537 0.88 -1.3 6,354,113 + -2.3 6,354,114 - -0.7 6,354,149 + -2.0 6,354,295 + -0.2 6,354,295 + -0.1 6,354,302 + -0.2 6,354,302 + +0.6 6,354,307 + -0.1 6,354,307 + -0.1 6,354,307 + +1.0 6,354,356 + Pf6N2E2_5538 0.10 -0.9 6,354,356 + Pf6N2E2_5538 0.10 +0.3 6,354,359 + Pf6N2E2_5538 0.11 -2.6 6,354,359 + Pf6N2E2_5538 0.11 -1.1 6,354,389 + Pf6N2E2_5538 0.17 -0.6 6,354,389 + Pf6N2E2_5538 0.17 -0.4 6,354,389 + Pf6N2E2_5538 0.17 +1.0 6,354,392 + Pf6N2E2_5538 0.18 -0.7 6,354,392 + Pf6N2E2_5538 0.18 +1.0 6,354,392 + Pf6N2E2_5538 0.18 +0.1 6,354,392 + Pf6N2E2_5538 0.18 +0.1 6,354,401 + Pf6N2E2_5538 0.20 -1.4 6,354,401 + Pf6N2E2_5538 0.20 -1.4 6,354,412 + Pf6N2E2_5538 0.22 -0.4 6,354,552 + Pf6N2E2_5538 0.52 -0.4 6,354,552 + Pf6N2E2_5538 0.52 -0.9 6,354,673 + Pf6N2E2_5538 0.78 -0.4 6,354,673 + Pf6N2E2_5538 0.78 +0.2 6,354,705 + Pf6N2E2_5538 0.85 -1.4 6,354,705 + Pf6N2E2_5538 0.85 -0.5 6,354,739 + -1.0 6,355,436 + -0.9 6,355,436 + +0.4 6,355,458 + +0.9 6,355,458 + +0.8 6,355,458 + -1.3
Or see this region's nucleotide sequence