Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4716 and Pf6N2E2_4717 are separated by 308 nucleotides Pf6N2E2_4717 and Pf6N2E2_4718 are separated by 21 nucleotides
Pf6N2E2_4716 - Membrane protein, putative, at 5,452,907 to 5,453,611
_4716
Pf6N2E2_4717 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 5,453,920 to 5,455,173
_4717
Pf6N2E2_4718 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 5,455,195 to 5,456,445
_4718
Position (kb)
5453
5454
5455
5456 Strain fitness (log2 ratio)
-2
-1
0
1 at 5452.983 kb on - strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.119 kb on - strand, within Pf6N2E2_4716 at 5453.119 kb on - strand, within Pf6N2E2_4716 at 5453.145 kb on - strand, within Pf6N2E2_4716 at 5453.272 kb on - strand, within Pf6N2E2_4716 at 5453.272 kb on - strand, within Pf6N2E2_4716 at 5453.364 kb on - strand, within Pf6N2E2_4716 at 5453.364 kb on - strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.421 kb on - strand, within Pf6N2E2_4716 at 5453.552 kb on + strand at 5453.673 kb on - strand at 5453.784 kb on + strand at 5453.837 kb on + strand at 5453.838 kb on - strand at 5453.838 kb on - strand at 5453.838 kb on - strand at 5453.951 kb on - strand at 5454.032 kb on - strand at 5454.082 kb on + strand, within Pf6N2E2_4717 at 5454.112 kb on + strand, within Pf6N2E2_4717 at 5454.112 kb on + strand, within Pf6N2E2_4717 at 5454.113 kb on - strand, within Pf6N2E2_4717 at 5454.233 kb on + strand, within Pf6N2E2_4717 at 5454.233 kb on + strand, within Pf6N2E2_4717 at 5454.234 kb on - strand, within Pf6N2E2_4717 at 5454.234 kb on - strand, within Pf6N2E2_4717 at 5454.236 kb on - strand, within Pf6N2E2_4717 at 5454.337 kb on + strand, within Pf6N2E2_4717 at 5454.337 kb on + strand, within Pf6N2E2_4717 at 5454.338 kb on - strand, within Pf6N2E2_4717 at 5454.352 kb on + strand, within Pf6N2E2_4717 at 5454.385 kb on + strand, within Pf6N2E2_4717 at 5454.452 kb on - strand, within Pf6N2E2_4717 at 5454.845 kb on + strand, within Pf6N2E2_4717 at 5454.845 kb on + strand, within Pf6N2E2_4717 at 5454.845 kb on + strand, within Pf6N2E2_4717 at 5454.845 kb on + strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5455.136 kb on + strand at 5455.187 kb on + strand at 5455.187 kb on + strand at 5455.188 kb on - strand at 5455.194 kb on + strand at 5455.194 kb on + strand at 5455.194 kb on + strand at 5455.194 kb on + strand at 5455.195 kb on - strand at 5455.195 kb on - strand at 5455.195 kb on - strand at 5455.204 kb on + strand at 5455.269 kb on + strand at 5455.291 kb on + strand at 5455.339 kb on + strand, within Pf6N2E2_4718 at 5455.339 kb on + strand, within Pf6N2E2_4718 at 5455.339 kb on + strand, within Pf6N2E2_4718 at 5455.340 kb on - strand, within Pf6N2E2_4718 at 5455.444 kb on + strand, within Pf6N2E2_4718 at 5455.445 kb on - strand, within Pf6N2E2_4718 at 5455.445 kb on - strand, within Pf6N2E2_4718 at 5455.445 kb on - strand, within Pf6N2E2_4718 at 5455.471 kb on + strand, within Pf6N2E2_4718 at 5455.472 kb on - strand, within Pf6N2E2_4718 at 5455.520 kb on - strand, within Pf6N2E2_4718 at 5455.549 kb on + strand, within Pf6N2E2_4718 at 5455.622 kb on + strand, within Pf6N2E2_4718 at 5455.623 kb on - strand, within Pf6N2E2_4718 at 5456.041 kb on + strand, within Pf6N2E2_4718 at 5456.095 kb on + strand, within Pf6N2E2_4718
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 5,452,983 - Pf6N2E2_4716 0.11 +0.4 5,453,009 + Pf6N2E2_4716 0.14 -0.2 5,453,009 + Pf6N2E2_4716 0.14 +1.2 5,453,009 + Pf6N2E2_4716 0.14 +1.4 5,453,009 + Pf6N2E2_4716 0.14 -0.6 5,453,009 + Pf6N2E2_4716 0.14 -0.4 5,453,010 - Pf6N2E2_4716 0.15 +0.9 5,453,010 - Pf6N2E2_4716 0.15 -0.8 5,453,010 - Pf6N2E2_4716 0.15 -1.1 5,453,010 - Pf6N2E2_4716 0.15 +0.1 5,453,010 - Pf6N2E2_4716 0.15 +0.1 5,453,010 - Pf6N2E2_4716 0.15 -0.0 5,453,010 - Pf6N2E2_4716 0.15 +0.8 5,453,010 - Pf6N2E2_4716 0.15 +0.5 5,453,119 - Pf6N2E2_4716 0.30 +1.3 5,453,119 - Pf6N2E2_4716 0.30 -0.0 5,453,145 - Pf6N2E2_4716 0.34 +0.9 5,453,272 - Pf6N2E2_4716 0.52 -1.3 5,453,272 - Pf6N2E2_4716 0.52 -2.5 5,453,364 - Pf6N2E2_4716 0.65 -0.5 5,453,364 - Pf6N2E2_4716 0.65 -0.3 5,453,420 + Pf6N2E2_4716 0.73 -1.8 5,453,420 + Pf6N2E2_4716 0.73 -0.1 5,453,420 + Pf6N2E2_4716 0.73 +0.5 5,453,421 - Pf6N2E2_4716 0.73 -0.3 5,453,552 + +0.6 5,453,673 - +0.2 5,453,784 + -0.1 5,453,837 + -1.6 5,453,838 - -1.7 5,453,838 - +0.4 5,453,838 - +0.5 5,453,951 - +0.8 5,454,032 - +0.5 5,454,082 + Pf6N2E2_4717 0.13 -0.7 5,454,112 + Pf6N2E2_4717 0.15 +1.0 5,454,112 + Pf6N2E2_4717 0.15 -0.9 5,454,113 - Pf6N2E2_4717 0.15 -0.5 5,454,233 + Pf6N2E2_4717 0.25 -0.2 5,454,233 + Pf6N2E2_4717 0.25 -0.4 5,454,234 - Pf6N2E2_4717 0.25 +0.3 5,454,234 - Pf6N2E2_4717 0.25 -1.7 5,454,236 - Pf6N2E2_4717 0.25 +0.3 5,454,337 + Pf6N2E2_4717 0.33 +0.4 5,454,337 + Pf6N2E2_4717 0.33 +0.7 5,454,338 - Pf6N2E2_4717 0.33 -0.8 5,454,352 + Pf6N2E2_4717 0.34 -1.1 5,454,385 + Pf6N2E2_4717 0.37 -1.3 5,454,452 - Pf6N2E2_4717 0.42 -0.6 5,454,845 + Pf6N2E2_4717 0.74 -0.4 5,454,845 + Pf6N2E2_4717 0.74 -1.2 5,454,845 + Pf6N2E2_4717 0.74 +0.5 5,454,845 + Pf6N2E2_4717 0.74 -1.2 5,454,846 - Pf6N2E2_4717 0.74 +0.1 5,454,846 - Pf6N2E2_4717 0.74 -0.7 5,454,846 - Pf6N2E2_4717 0.74 +0.3 5,454,846 - Pf6N2E2_4717 0.74 +0.2 5,454,846 - Pf6N2E2_4717 0.74 -0.5 5,455,136 + +0.5 5,455,187 + +0.2 5,455,187 + -0.6 5,455,188 - -0.7 5,455,194 + +1.0 5,455,194 + -0.2 5,455,194 + +0.2 5,455,194 + +0.7 5,455,195 - +0.6 5,455,195 - -1.4 5,455,195 - +0.2 5,455,204 + +0.5 5,455,269 + +0.6 5,455,291 + -0.4 5,455,339 + Pf6N2E2_4718 0.12 -0.1 5,455,339 + Pf6N2E2_4718 0.12 +0.7 5,455,339 + Pf6N2E2_4718 0.12 -1.6 5,455,340 - Pf6N2E2_4718 0.12 +0.2 5,455,444 + Pf6N2E2_4718 0.20 +0.5 5,455,445 - Pf6N2E2_4718 0.20 +0.1 5,455,445 - Pf6N2E2_4718 0.20 +0.0 5,455,445 - Pf6N2E2_4718 0.20 -1.1 5,455,471 + Pf6N2E2_4718 0.22 -0.8 5,455,472 - Pf6N2E2_4718 0.22 -0.6 5,455,520 - Pf6N2E2_4718 0.26 +0.5 5,455,549 + Pf6N2E2_4718 0.28 -2.4 5,455,622 + Pf6N2E2_4718 0.34 -0.1 5,455,623 - Pf6N2E2_4718 0.34 +0.6 5,456,041 + Pf6N2E2_4718 0.68 -0.7 5,456,095 + Pf6N2E2_4718 0.72 -1.3
Or see this region's nucleotide sequence