Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_462 and Pf6N2E2_463 are separated by 167 nucleotides Pf6N2E2_463 and Pf6N2E2_464 are separated by 4 nucleotides Pf6N2E2_464 and Pf6N2E2_465 are separated by 68 nucleotides Pf6N2E2_465 and Pf6N2E2_466 are separated by 41 nucleotides
Pf6N2E2_462 - Aspartate aminotransferase (EC 2.6.1.1), at 527,302 to 528,486
_462
Pf6N2E2_463 - Transcriptional regulatory protein RstA, at 528,654 to 529,355
_463
Pf6N2E2_464 - Sensory histidine kinase QseC, at 529,360 to 530,661
_464
Pf6N2E2_465 - Probable transmembrane protein, at 530,730 to 531,371
_465
Pf6N2E2_466 - Nicotinamidase (EC 3.5.1.19), at 531,413 to 532,066
_466
Position (kb) 529
530
531 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 528.391 kb on - strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.548 kb on + strand at 528.711 kb on + strand at 528.712 kb on - strand at 528.948 kb on + strand, within Pf6N2E2_463 at 528.948 kb on + strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.995 kb on + strand, within Pf6N2E2_463 at 529.131 kb on + strand, within Pf6N2E2_463 at 529.132 kb on - strand, within Pf6N2E2_463 at 529.132 kb on - strand, within Pf6N2E2_463 at 529.171 kb on + strand, within Pf6N2E2_463 at 529.171 kb on + strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.406 kb on - strand at 529.420 kb on + strand at 529.421 kb on - strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.472 kb on - strand at 529.484 kb on - strand at 529.774 kb on + strand, within Pf6N2E2_464 at 529.775 kb on - strand, within Pf6N2E2_464 at 529.775 kb on - strand, within Pf6N2E2_464 at 530.732 kb on - strand at 530.754 kb on - strand at 530.890 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.906 kb on - strand, within Pf6N2E2_465 at 530.906 kb on - strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.966 kb on - strand, within Pf6N2E2_465 at 530.968 kb on + strand, within Pf6N2E2_465 at 530.968 kb on + strand, within Pf6N2E2_465 at 530.969 kb on - strand, within Pf6N2E2_465 at 530.969 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.404 kb on + strand at 531.404 kb on + strand at 531.405 kb on - strand at 531.405 kb on - strand at 531.434 kb on + strand at 531.435 kb on - strand at 531.435 kb on - strand at 531.615 kb on + strand, within Pf6N2E2_466 at 531.615 kb on + strand, within Pf6N2E2_466 at 531.616 kb on - strand, within Pf6N2E2_466
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 528,391 - +1.0 528,546 + +0.2 528,546 + +0.9 528,546 + -0.9 528,546 + +1.3 528,546 + +0.7 528,548 + -2.3 528,711 + -0.3 528,712 - +0.3 528,948 + Pf6N2E2_463 0.42 -0.0 528,948 + Pf6N2E2_463 0.42 +1.0 528,949 - Pf6N2E2_463 0.42 +0.8 528,949 - Pf6N2E2_463 0.42 -0.2 528,949 - Pf6N2E2_463 0.42 -0.6 528,949 - Pf6N2E2_463 0.42 +0.2 528,949 - Pf6N2E2_463 0.42 +0.6 528,995 + Pf6N2E2_463 0.49 +0.5 529,131 + Pf6N2E2_463 0.68 +0.6 529,132 - Pf6N2E2_463 0.68 -0.3 529,132 - Pf6N2E2_463 0.68 -0.4 529,171 + Pf6N2E2_463 0.74 -0.2 529,171 + Pf6N2E2_463 0.74 -0.2 529,172 - Pf6N2E2_463 0.74 +0.3 529,172 - Pf6N2E2_463 0.74 -0.4 529,172 - Pf6N2E2_463 0.74 -0.2 529,406 - +0.8 529,420 + -1.0 529,421 - -0.2 529,471 + +0.1 529,471 + +0.8 529,471 + +0.4 529,471 + -1.0 529,471 + -1.1 529,471 + +0.6 529,472 - +1.0 529,484 - -0.2 529,774 + Pf6N2E2_464 0.32 -1.0 529,775 - Pf6N2E2_464 0.32 -0.5 529,775 - Pf6N2E2_464 0.32 -1.2 530,732 - -0.2 530,754 - +0.3 530,890 + Pf6N2E2_465 0.25 -0.1 530,905 + Pf6N2E2_465 0.27 +1.4 530,905 + Pf6N2E2_465 0.27 +1.3 530,905 + Pf6N2E2_465 0.27 -1.9 530,906 - Pf6N2E2_465 0.27 -2.9 530,906 - Pf6N2E2_465 0.27 +0.2 530,965 + Pf6N2E2_465 0.37 +0.8 530,965 + Pf6N2E2_465 0.37 -0.8 530,965 + Pf6N2E2_465 0.37 +0.6 530,965 + Pf6N2E2_465 0.37 +1.5 530,966 - Pf6N2E2_465 0.37 +0.5 530,968 + Pf6N2E2_465 0.37 -0.4 530,968 + Pf6N2E2_465 0.37 -1.4 530,969 - Pf6N2E2_465 0.37 +0.8 530,969 - Pf6N2E2_465 0.37 +0.2 531,239 - Pf6N2E2_465 0.79 +0.2 531,239 - Pf6N2E2_465 0.79 +0.2 531,239 - Pf6N2E2_465 0.79 +0.5 531,401 + +0.7 531,401 + +0.3 531,401 + -0.4 531,401 + -0.6 531,401 + -0.8 531,404 + +2.1 531,404 + -0.0 531,405 - -0.0 531,405 - -1.0 531,434 + +0.0 531,435 - +1.1 531,435 - -0.1 531,615 + Pf6N2E2_466 0.31 -0.0 531,615 + Pf6N2E2_466 0.31 +1.0 531,616 - Pf6N2E2_466 0.31 +0.5
Or see this region's nucleotide sequence