Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3132 and Pf6N2E2_3133 overlap by 38 nucleotides Pf6N2E2_3133 and Pf6N2E2_3134 are separated by 44 nucleotides Pf6N2E2_3134 and Pf6N2E2_3135 are separated by 361 nucleotides Pf6N2E2_3135 and Pf6N2E2_3136 are separated by 113 nucleotides
Pf6N2E2_3132 - FIG00954548: hypothetical protein, at 3,624,072 to 3,624,335
_3132
Pf6N2E2_3133 - Death on curing protein, Doc toxin, at 3,624,298 to 3,624,597
_3133
Pf6N2E2_3134 - hypothetical protein, at 3,624,642 to 3,624,827
_3134
Pf6N2E2_3135 - Glucans biosynthesis protein D precursor, at 3,625,189 to 3,626,817
_3135
Pf6N2E2_3136 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 3,626,931 to 3,628,037
_3136
Position (kb)
3625
3626
3627 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3624.190 kb on - strand, within Pf6N2E2_3132 at 3624.190 kb on - strand, within Pf6N2E2_3132 at 3624.190 kb on - strand, within Pf6N2E2_3132 at 3624.284 kb on + strand, within Pf6N2E2_3132 at 3624.284 kb on + strand, within Pf6N2E2_3132 at 3624.284 kb on + strand, within Pf6N2E2_3132 at 3624.311 kb on + strand at 3624.311 kb on + strand at 3624.312 kb on - strand at 3624.680 kb on - strand, within Pf6N2E2_3134 at 3624.816 kb on + strand at 3624.842 kb on + strand at 3624.842 kb on + strand at 3624.842 kb on + strand at 3624.907 kb on - strand at 3624.938 kb on + strand at 3624.943 kb on + strand at 3624.972 kb on + strand at 3624.973 kb on - strand at 3624.973 kb on - strand at 3624.984 kb on + strand at 3624.984 kb on + strand at 3624.985 kb on - strand at 3624.985 kb on - strand at 3625.035 kb on + strand at 3625.035 kb on + strand at 3625.036 kb on - strand at 3625.045 kb on - strand at 3625.083 kb on + strand at 3625.083 kb on + strand at 3625.083 kb on + strand at 3625.083 kb on + strand at 3625.083 kb on + strand at 3625.083 kb on + strand at 3625.083 kb on + strand at 3625.083 kb on + strand at 3625.084 kb on - strand at 3625.084 kb on - strand at 3625.092 kb on + strand at 3625.092 kb on + strand at 3625.092 kb on + strand at 3625.092 kb on + strand at 3625.092 kb on + strand at 3625.093 kb on - strand at 3625.093 kb on - strand at 3625.093 kb on - strand at 3625.135 kb on - strand at 3625.184 kb on + strand at 3625.184 kb on + strand at 3625.184 kb on + strand at 3625.184 kb on + strand at 3625.185 kb on - strand at 3625.185 kb on - strand at 3625.185 kb on - strand at 3625.185 kb on - strand at 3625.185 kb on - strand at 3625.185 kb on - strand at 3625.185 kb on - strand at 3625.185 kb on - strand at 3625.188 kb on + strand at 3625.188 kb on + strand at 3625.188 kb on + strand at 3625.188 kb on + strand at 3625.189 kb on - strand at 3625.189 kb on - strand at 3625.189 kb on - strand at 3625.189 kb on - strand at 3625.189 kb on - strand at 3625.235 kb on - strand at 3625.439 kb on - strand, within Pf6N2E2_3135 at 3625.537 kb on + strand, within Pf6N2E2_3135 at 3625.537 kb on + strand, within Pf6N2E2_3135 at 3625.537 kb on + strand, within Pf6N2E2_3135 at 3625.537 kb on + strand, within Pf6N2E2_3135 at 3625.537 kb on + strand, within Pf6N2E2_3135 at 3625.537 kb on + strand, within Pf6N2E2_3135 at 3625.538 kb on - strand, within Pf6N2E2_3135 at 3625.538 kb on - strand, within Pf6N2E2_3135 at 3625.538 kb on - strand, within Pf6N2E2_3135 at 3625.538 kb on - strand, within Pf6N2E2_3135 at 3625.538 kb on - strand, within Pf6N2E2_3135 at 3625.538 kb on - strand, within Pf6N2E2_3135 at 3625.538 kb on - strand, within Pf6N2E2_3135 at 3625.560 kb on + strand, within Pf6N2E2_3135 at 3625.561 kb on - strand, within Pf6N2E2_3135 at 3625.601 kb on - strand, within Pf6N2E2_3135 at 3625.720 kb on + strand, within Pf6N2E2_3135 at 3625.720 kb on + strand, within Pf6N2E2_3135 at 3625.786 kb on + strand, within Pf6N2E2_3135 at 3625.786 kb on + strand, within Pf6N2E2_3135 at 3625.871 kb on - strand, within Pf6N2E2_3135 at 3625.871 kb on - strand, within Pf6N2E2_3135 at 3626.222 kb on - strand, within Pf6N2E2_3135 at 3626.243 kb on - strand, within Pf6N2E2_3135 at 3626.319 kb on + strand, within Pf6N2E2_3135 at 3626.319 kb on + strand, within Pf6N2E2_3135 at 3626.320 kb on - strand, within Pf6N2E2_3135 at 3626.398 kb on + strand, within Pf6N2E2_3135 at 3626.398 kb on + strand, within Pf6N2E2_3135 at 3626.398 kb on + strand, within Pf6N2E2_3135 at 3626.398 kb on + strand, within Pf6N2E2_3135 at 3626.398 kb on + strand, within Pf6N2E2_3135 at 3626.399 kb on - strand, within Pf6N2E2_3135 at 3626.399 kb on - strand, within Pf6N2E2_3135 at 3626.399 kb on - strand, within Pf6N2E2_3135 at 3626.399 kb on - strand, within Pf6N2E2_3135 at 3626.399 kb on - strand, within Pf6N2E2_3135 at 3626.399 kb on - strand, within Pf6N2E2_3135 at 3626.399 kb on - strand, within Pf6N2E2_3135 at 3626.404 kb on + strand, within Pf6N2E2_3135 at 3626.669 kb on - strand at 3626.677 kb on - strand at 3626.773 kb on + strand at 3626.773 kb on + strand at 3626.773 kb on + strand at 3626.773 kb on + strand at 3626.773 kb on + strand at 3626.779 kb on + strand at 3626.779 kb on + strand at 3626.779 kb on + strand at 3626.780 kb on - strand at 3626.780 kb on - strand at 3626.809 kb on + strand at 3626.809 kb on + strand at 3626.809 kb on + strand at 3626.810 kb on - strand at 3626.810 kb on - strand at 3626.810 kb on - strand at 3626.857 kb on + strand at 3626.858 kb on - strand at 3626.921 kb on + strand at 3627.084 kb on - strand, within Pf6N2E2_3136 at 3627.378 kb on + strand, within Pf6N2E2_3136 at 3627.443 kb on - strand, within Pf6N2E2_3136 at 3627.494 kb on - strand, within Pf6N2E2_3136 at 3627.678 kb on + strand, within Pf6N2E2_3136 at 3627.678 kb on + strand, within Pf6N2E2_3136 at 3627.678 kb on - strand, within Pf6N2E2_3136 at 3627.679 kb on - strand, within Pf6N2E2_3136 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.745 kb on - strand, within Pf6N2E2_3136 at 3627.745 kb on - strand, within Pf6N2E2_3136
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 3,624,190 - Pf6N2E2_3132 0.45 -0.4 3,624,190 - Pf6N2E2_3132 0.45 +0.2 3,624,190 - Pf6N2E2_3132 0.45 -1.1 3,624,284 + Pf6N2E2_3132 0.80 -2.2 3,624,284 + Pf6N2E2_3132 0.80 -1.1 3,624,284 + Pf6N2E2_3132 0.80 +1.8 3,624,311 + +0.5 3,624,311 + +0.4 3,624,312 - +0.7 3,624,680 - Pf6N2E2_3134 0.20 +1.1 3,624,816 + -0.6 3,624,842 + -0.2 3,624,842 + +0.7 3,624,842 + +1.1 3,624,907 - +0.4 3,624,938 + +0.6 3,624,943 + -1.4 3,624,972 + -0.2 3,624,973 - +0.1 3,624,973 - +1.6 3,624,984 + +2.4 3,624,984 + -0.6 3,624,985 - +0.7 3,624,985 - +1.4 3,625,035 + +0.5 3,625,035 + -0.6 3,625,036 - +1.4 3,625,045 - +2.0 3,625,083 + +0.4 3,625,083 + +0.8 3,625,083 + +2.2 3,625,083 + +0.6 3,625,083 + -0.6 3,625,083 + +0.7 3,625,083 + +0.5 3,625,083 + +0.1 3,625,084 - +1.3 3,625,084 - +0.9 3,625,092 + -0.4 3,625,092 + +0.2 3,625,092 + +0.8 3,625,092 + +0.8 3,625,092 + +1.4 3,625,093 - +1.3 3,625,093 - +0.6 3,625,093 - +1.6 3,625,135 - +1.5 3,625,184 + +1.7 3,625,184 + +2.1 3,625,184 + +2.1 3,625,184 + +0.3 3,625,185 - -0.3 3,625,185 - +0.7 3,625,185 - +2.9 3,625,185 - +1.2 3,625,185 - +0.8 3,625,185 - +1.5 3,625,185 - +2.2 3,625,185 - +1.3 3,625,188 + +2.6 3,625,188 + +2.5 3,625,188 + +3.4 3,625,188 + -0.4 3,625,189 - +2.2 3,625,189 - +2.2 3,625,189 - +0.5 3,625,189 - +2.5 3,625,189 - -0.0 3,625,235 - +1.7 3,625,439 - Pf6N2E2_3135 0.15 +2.1 3,625,537 + Pf6N2E2_3135 0.21 +1.2 3,625,537 + Pf6N2E2_3135 0.21 +3.0 3,625,537 + Pf6N2E2_3135 0.21 +1.6 3,625,537 + Pf6N2E2_3135 0.21 +1.8 3,625,537 + Pf6N2E2_3135 0.21 +1.5 3,625,537 + Pf6N2E2_3135 0.21 +1.7 3,625,538 - Pf6N2E2_3135 0.21 +1.5 3,625,538 - Pf6N2E2_3135 0.21 +2.6 3,625,538 - Pf6N2E2_3135 0.21 +1.9 3,625,538 - Pf6N2E2_3135 0.21 +1.8 3,625,538 - Pf6N2E2_3135 0.21 +1.9 3,625,538 - Pf6N2E2_3135 0.21 +1.1 3,625,538 - Pf6N2E2_3135 0.21 +1.3 3,625,560 + Pf6N2E2_3135 0.23 +1.2 3,625,561 - Pf6N2E2_3135 0.23 +0.7 3,625,601 - Pf6N2E2_3135 0.25 +2.0 3,625,720 + Pf6N2E2_3135 0.33 +0.8 3,625,720 + Pf6N2E2_3135 0.33 +3.0 3,625,786 + Pf6N2E2_3135 0.37 +1.5 3,625,786 + Pf6N2E2_3135 0.37 +1.8 3,625,871 - Pf6N2E2_3135 0.42 +0.9 3,625,871 - Pf6N2E2_3135 0.42 +2.7 3,626,222 - Pf6N2E2_3135 0.63 +2.8 3,626,243 - Pf6N2E2_3135 0.65 +0.6 3,626,319 + Pf6N2E2_3135 0.69 +2.1 3,626,319 + Pf6N2E2_3135 0.69 +1.8 3,626,320 - Pf6N2E2_3135 0.69 +3.3 3,626,398 + Pf6N2E2_3135 0.74 +1.8 3,626,398 + Pf6N2E2_3135 0.74 +2.1 3,626,398 + Pf6N2E2_3135 0.74 +1.0 3,626,398 + Pf6N2E2_3135 0.74 +1.8 3,626,398 + Pf6N2E2_3135 0.74 +1.8 3,626,399 - Pf6N2E2_3135 0.74 +1.3 3,626,399 - Pf6N2E2_3135 0.74 +2.1 3,626,399 - Pf6N2E2_3135 0.74 +1.5 3,626,399 - Pf6N2E2_3135 0.74 +2.7 3,626,399 - Pf6N2E2_3135 0.74 +0.4 3,626,399 - Pf6N2E2_3135 0.74 +2.9 3,626,399 - Pf6N2E2_3135 0.74 +1.5 3,626,404 + Pf6N2E2_3135 0.75 +2.0 3,626,669 - +1.6 3,626,677 - +1.5 3,626,773 + +2.6 3,626,773 + +1.6 3,626,773 + +1.9 3,626,773 + +1.4 3,626,773 + +2.6 3,626,779 + +2.0 3,626,779 + +1.2 3,626,779 + +2.0 3,626,780 - +1.5 3,626,780 - +3.2 3,626,809 + +1.0 3,626,809 + +0.9 3,626,809 + +0.2 3,626,810 - -0.5 3,626,810 - +0.8 3,626,810 - +1.0 3,626,857 + +0.2 3,626,858 - -0.6 3,626,921 + -0.5 3,627,084 - Pf6N2E2_3136 0.14 +0.5 3,627,378 + Pf6N2E2_3136 0.40 +0.1 3,627,443 - Pf6N2E2_3136 0.46 +0.4 3,627,494 - Pf6N2E2_3136 0.51 +0.7 3,627,678 + Pf6N2E2_3136 0.67 +0.8 3,627,678 + Pf6N2E2_3136 0.67 -0.6 3,627,678 - Pf6N2E2_3136 0.67 +2.1 3,627,679 - Pf6N2E2_3136 0.68 -0.7 3,627,744 + Pf6N2E2_3136 0.73 +1.0 3,627,744 + Pf6N2E2_3136 0.73 +0.6 3,627,744 + Pf6N2E2_3136 0.73 -0.5 3,627,744 + Pf6N2E2_3136 0.73 +1.5 3,627,744 + Pf6N2E2_3136 0.73 +0.4 3,627,745 - Pf6N2E2_3136 0.74 -3.1 3,627,745 - Pf6N2E2_3136 0.74 -0.4
Or see this region's nucleotide sequence