Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3064 and Pf6N2E2_3065 overlap by 1 nucleotides Pf6N2E2_3065 and Pf6N2E2_3066 are separated by 13 nucleotides Pf6N2E2_3066 and Pf6N2E2_3067 overlap by 4 nucleotides Pf6N2E2_3067 and Pf6N2E2_3068 are separated by 213 nucleotides
Pf6N2E2_3064 - Phosphatidate cytidylyltransferase (EC 2.7.7.41), at 3,551,349 to 3,552,155
_3064
Pf6N2E2_3065 - Undecaprenyl diphosphate synthase (EC 2.5.1.31), at 3,552,155 to 3,552,910
_3065
Pf6N2E2_3066 - Ribosome recycling factor, at 3,552,924 to 3,553,481
_3066
Pf6N2E2_3067 - Uridine monophosphate kinase (EC 2.7.4.22), at 3,553,478 to 3,554,221
_3067
Pf6N2E2_3068 - Translation elongation factor Ts, at 3,554,435 to 3,555,301
_3068
Position (kb)
3552
3553
3554 Strain fitness (log2 ratio)
-1
0
1
2 at 3552.926 kb on - strand at 3552.926 kb on - strand at 3552.928 kb on - strand at 3552.928 kb on - strand at 3554.284 kb on - strand at 3554.289 kb on - strand at 3554.329 kb on - strand at 3554.437 kb on - strand at 3554.437 kb on - strand at 3554.437 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 3,552,926 - -0.9 3,552,926 - -1.6 3,552,928 - -0.9 3,552,928 - +0.8 3,554,284 - +2.7 3,554,289 - +1.4 3,554,329 - +0.9 3,554,437 - -0.5 3,554,437 - -1.6 3,554,437 - -0.3
Or see this region's nucleotide sequence