Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3006 and Pf6N2E2_3007 overlap by 14 nucleotides Pf6N2E2_3007 and Pf6N2E2_3008 are separated by 130 nucleotides Pf6N2E2_3008 and Pf6N2E2_3009 overlap by 4 nucleotides Pf6N2E2_3009 and Pf6N2E2_3010 are separated by 67 nucleotides
Pf6N2E2_3006 - Phage tail/DNA circulation protein, at 3,495,088 to 3,496,326
_3006
Pf6N2E2_3007 - Phage tail length tape-measure protein, at 3,496,313 to 3,497,887
_3007
Pf6N2E2_3008 - FIG00954242: hypothetical protein, at 3,498,018 to 3,498,314
_3008
Pf6N2E2_3009 - Phage tail tube protein, at 3,498,311 to 3,498,658
_3009
Pf6N2E2_3010 - Bacteriophage tail sheath protein, at 3,498,726 to 3,500,222
_3010
Position (kb)
3496
3497
3498 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3495.315 kb on - strand, within Pf6N2E2_3006 at 3495.529 kb on + strand, within Pf6N2E2_3006 at 3495.765 kb on - strand, within Pf6N2E2_3006 at 3495.807 kb on - strand, within Pf6N2E2_3006 at 3495.807 kb on - strand, within Pf6N2E2_3006 at 3495.980 kb on + strand, within Pf6N2E2_3006 at 3496.520 kb on - strand, within Pf6N2E2_3007 at 3496.520 kb on - strand, within Pf6N2E2_3007 at 3497.198 kb on - strand, within Pf6N2E2_3007 at 3497.453 kb on + strand, within Pf6N2E2_3007 at 3497.453 kb on + strand, within Pf6N2E2_3007 at 3497.454 kb on - strand, within Pf6N2E2_3007 at 3497.454 kb on - strand, within Pf6N2E2_3007 at 3497.609 kb on + strand, within Pf6N2E2_3007 at 3497.664 kb on - strand, within Pf6N2E2_3007 at 3497.705 kb on - strand, within Pf6N2E2_3007 at 3497.705 kb on - strand, within Pf6N2E2_3007 at 3497.746 kb on + strand at 3497.803 kb on + strand at 3497.804 kb on - strand at 3498.005 kb on + strand at 3498.005 kb on + strand at 3498.145 kb on + strand, within Pf6N2E2_3008 at 3498.145 kb on + strand, within Pf6N2E2_3008 at 3498.145 kb on + strand, within Pf6N2E2_3008 at 3498.145 kb on + strand, within Pf6N2E2_3008 at 3498.145 kb on + strand, within Pf6N2E2_3008 at 3498.145 kb on + strand, within Pf6N2E2_3008 at 3498.146 kb on - strand, within Pf6N2E2_3008 at 3498.146 kb on - strand, within Pf6N2E2_3008 at 3498.146 kb on - strand, within Pf6N2E2_3008 at 3498.411 kb on + strand, within Pf6N2E2_3009 at 3498.411 kb on + strand, within Pf6N2E2_3009 at 3498.412 kb on - strand, within Pf6N2E2_3009 at 3498.537 kb on + strand, within Pf6N2E2_3009 at 3498.537 kb on + strand, within Pf6N2E2_3009 at 3498.538 kb on - strand, within Pf6N2E2_3009 at 3498.630 kb on + strand at 3498.630 kb on + strand at 3498.631 kb on - strand at 3498.631 kb on - strand at 3498.631 kb on - strand at 3498.678 kb on - strand at 3498.721 kb on + strand at 3498.722 kb on - strand at 3498.722 kb on - strand at 3498.742 kb on + strand at 3498.743 kb on - strand at 3498.793 kb on + strand at 3498.794 kb on - strand at 3498.794 kb on - strand at 3498.794 kb on - strand at 3498.794 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 3,495,315 - Pf6N2E2_3006 0.18 -1.1 3,495,529 + Pf6N2E2_3006 0.36 -0.9 3,495,765 - Pf6N2E2_3006 0.55 +0.0 3,495,807 - Pf6N2E2_3006 0.58 -0.1 3,495,807 - Pf6N2E2_3006 0.58 -0.9 3,495,980 + Pf6N2E2_3006 0.72 +1.2 3,496,520 - Pf6N2E2_3007 0.13 -0.7 3,496,520 - Pf6N2E2_3007 0.13 -0.8 3,497,198 - Pf6N2E2_3007 0.56 -1.4 3,497,453 + Pf6N2E2_3007 0.72 +0.5 3,497,453 + Pf6N2E2_3007 0.72 -0.8 3,497,454 - Pf6N2E2_3007 0.72 +0.5 3,497,454 - Pf6N2E2_3007 0.72 -0.9 3,497,609 + Pf6N2E2_3007 0.82 -0.7 3,497,664 - Pf6N2E2_3007 0.86 -2.0 3,497,705 - Pf6N2E2_3007 0.88 +0.5 3,497,705 - Pf6N2E2_3007 0.88 -1.3 3,497,746 + -0.2 3,497,803 + +1.1 3,497,804 - -0.3 3,498,005 + +0.1 3,498,005 + +0.7 3,498,145 + Pf6N2E2_3008 0.43 +0.3 3,498,145 + Pf6N2E2_3008 0.43 +1.6 3,498,145 + Pf6N2E2_3008 0.43 +0.2 3,498,145 + Pf6N2E2_3008 0.43 +0.0 3,498,145 + Pf6N2E2_3008 0.43 -0.3 3,498,145 + Pf6N2E2_3008 0.43 -0.7 3,498,146 - Pf6N2E2_3008 0.43 -0.3 3,498,146 - Pf6N2E2_3008 0.43 +0.8 3,498,146 - Pf6N2E2_3008 0.43 -0.0 3,498,411 + Pf6N2E2_3009 0.29 +0.1 3,498,411 + Pf6N2E2_3009 0.29 +1.3 3,498,412 - Pf6N2E2_3009 0.29 -0.7 3,498,537 + Pf6N2E2_3009 0.65 -0.7 3,498,537 + Pf6N2E2_3009 0.65 -1.2 3,498,538 - Pf6N2E2_3009 0.65 +1.0 3,498,630 + -1.4 3,498,630 + -1.4 3,498,631 - +2.1 3,498,631 - -0.8 3,498,631 - +0.7 3,498,678 - +0.3 3,498,721 + +1.3 3,498,722 - +0.6 3,498,722 - -0.2 3,498,742 + +0.4 3,498,743 - -1.2 3,498,793 + -0.1 3,498,794 - +0.2 3,498,794 - -0.2 3,498,794 - +0.3 3,498,794 - -0.4
Or see this region's nucleotide sequence