Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2696 and Pf6N2E2_2697 are separated by 130 nucleotides Pf6N2E2_2697 and Pf6N2E2_2698 are separated by 119 nucleotides
Pf6N2E2_2696 - Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-), at 3,188,514 to 3,190,235
_2696
Pf6N2E2_2697 - Permease of the drug/metabolite transporter (DMT) superfamily, at 3,190,366 to 3,191,274
_2697
Pf6N2E2_2698 - Permease of the drug/metabolite transporter (DMT) superfamily, at 3,191,394 to 3,192,257
_2698
Position (kb)
3190
3191
3192 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3189.379 kb on + strand, within Pf6N2E2_2696 at 3189.379 kb on + strand, within Pf6N2E2_2696 at 3189.379 kb on + strand, within Pf6N2E2_2696 at 3189.380 kb on - strand, within Pf6N2E2_2696 at 3189.380 kb on - strand, within Pf6N2E2_2696 at 3189.463 kb on + strand, within Pf6N2E2_2696 at 3189.464 kb on - strand, within Pf6N2E2_2696 at 3189.607 kb on + strand, within Pf6N2E2_2696 at 3189.608 kb on - strand, within Pf6N2E2_2696 at 3189.758 kb on - strand, within Pf6N2E2_2696 at 3189.808 kb on + strand, within Pf6N2E2_2696 at 3189.808 kb on + strand, within Pf6N2E2_2696 at 3189.809 kb on - strand, within Pf6N2E2_2696 at 3189.809 kb on - strand, within Pf6N2E2_2696 at 3189.809 kb on - strand, within Pf6N2E2_2696 at 3189.809 kb on - strand, within Pf6N2E2_2696 at 3189.809 kb on - strand, within Pf6N2E2_2696 at 3189.809 kb on - strand, within Pf6N2E2_2696 at 3189.923 kb on - strand, within Pf6N2E2_2696 at 3189.971 kb on + strand, within Pf6N2E2_2696 at 3189.972 kb on - strand, within Pf6N2E2_2696 at 3189.972 kb on - strand, within Pf6N2E2_2696 at 3189.972 kb on - strand, within Pf6N2E2_2696 at 3189.973 kb on + strand, within Pf6N2E2_2696 at 3189.973 kb on + strand, within Pf6N2E2_2696 at 3189.973 kb on + strand, within Pf6N2E2_2696 at 3189.974 kb on - strand, within Pf6N2E2_2696 at 3190.072 kb on + strand at 3190.072 kb on + strand at 3190.072 kb on + strand at 3190.072 kb on + strand at 3190.072 kb on + strand at 3190.073 kb on - strand at 3190.073 kb on - strand at 3190.073 kb on - strand at 3190.073 kb on - strand at 3190.073 kb on - strand at 3190.235 kb on + strand at 3190.236 kb on - strand at 3190.241 kb on - strand at 3190.241 kb on - strand at 3190.264 kb on + strand at 3190.280 kb on + strand at 3190.392 kb on - strand at 3190.609 kb on + strand, within Pf6N2E2_2697 at 3190.609 kb on + strand, within Pf6N2E2_2697 at 3190.609 kb on + strand, within Pf6N2E2_2697 at 3190.609 kb on + strand, within Pf6N2E2_2697 at 3190.610 kb on - strand, within Pf6N2E2_2697 at 3190.642 kb on + strand, within Pf6N2E2_2697 at 3190.846 kb on + strand, within Pf6N2E2_2697 at 3190.846 kb on + strand, within Pf6N2E2_2697 at 3190.847 kb on - strand, within Pf6N2E2_2697 at 3190.966 kb on + strand, within Pf6N2E2_2697 at 3191.059 kb on + strand, within Pf6N2E2_2697 at 3191.060 kb on - strand, within Pf6N2E2_2697 at 3191.060 kb on - strand, within Pf6N2E2_2697 at 3191.060 kb on - strand, within Pf6N2E2_2697 at 3191.060 kb on - strand, within Pf6N2E2_2697 at 3191.068 kb on + strand, within Pf6N2E2_2697 at 3191.069 kb on - strand, within Pf6N2E2_2697 at 3191.069 kb on - strand, within Pf6N2E2_2697 at 3191.191 kb on + strand at 3191.191 kb on + strand at 3191.191 kb on + strand at 3191.192 kb on - strand at 3191.192 kb on - strand at 3191.368 kb on + strand at 3191.369 kb on - strand at 3191.370 kb on + strand at 3191.370 kb on + strand at 3191.371 kb on - strand at 3191.406 kb on - strand at 3191.406 kb on - strand at 3191.488 kb on + strand, within Pf6N2E2_2698 at 3191.489 kb on - strand, within Pf6N2E2_2698 at 3191.521 kb on + strand, within Pf6N2E2_2698 at 3191.522 kb on - strand, within Pf6N2E2_2698 at 3191.522 kb on - strand, within Pf6N2E2_2698 at 3191.522 kb on - strand, within Pf6N2E2_2698 at 3191.522 kb on - strand, within Pf6N2E2_2698 at 3191.522 kb on - strand, within Pf6N2E2_2698 at 3191.570 kb on - strand, within Pf6N2E2_2698 at 3191.642 kb on - strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.837 kb on - strand, within Pf6N2E2_2698 at 3191.837 kb on - strand, within Pf6N2E2_2698 at 3192.261 kb on + strand at 3192.270 kb on + strand at 3192.270 kb on + strand at 3192.270 kb on + strand at 3192.271 kb on - strand at 3192.271 kb on - strand at 3192.271 kb on - strand at 3192.271 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 3,189,379 + Pf6N2E2_2696 0.50 -1.3 3,189,379 + Pf6N2E2_2696 0.50 +0.0 3,189,379 + Pf6N2E2_2696 0.50 -0.8 3,189,380 - Pf6N2E2_2696 0.50 +0.4 3,189,380 - Pf6N2E2_2696 0.50 +0.5 3,189,463 + Pf6N2E2_2696 0.55 -0.5 3,189,464 - Pf6N2E2_2696 0.55 +0.6 3,189,607 + Pf6N2E2_2696 0.63 -1.0 3,189,608 - Pf6N2E2_2696 0.64 -0.3 3,189,758 - Pf6N2E2_2696 0.72 -0.1 3,189,808 + Pf6N2E2_2696 0.75 +0.7 3,189,808 + Pf6N2E2_2696 0.75 -1.2 3,189,809 - Pf6N2E2_2696 0.75 +0.4 3,189,809 - Pf6N2E2_2696 0.75 -1.5 3,189,809 - Pf6N2E2_2696 0.75 +0.2 3,189,809 - Pf6N2E2_2696 0.75 -0.5 3,189,809 - Pf6N2E2_2696 0.75 +1.2 3,189,809 - Pf6N2E2_2696 0.75 +0.2 3,189,923 - Pf6N2E2_2696 0.82 -1.1 3,189,971 + Pf6N2E2_2696 0.85 -0.3 3,189,972 - Pf6N2E2_2696 0.85 +0.6 3,189,972 - Pf6N2E2_2696 0.85 -0.6 3,189,972 - Pf6N2E2_2696 0.85 +0.5 3,189,973 + Pf6N2E2_2696 0.85 -0.0 3,189,973 + Pf6N2E2_2696 0.85 +1.0 3,189,973 + Pf6N2E2_2696 0.85 +0.1 3,189,974 - Pf6N2E2_2696 0.85 +0.2 3,190,072 + +0.2 3,190,072 + -0.7 3,190,072 + +0.1 3,190,072 + -1.4 3,190,072 + +1.2 3,190,073 - +0.0 3,190,073 - -1.8 3,190,073 - +0.7 3,190,073 - -0.1 3,190,073 - -0.6 3,190,235 + +0.4 3,190,236 - -0.1 3,190,241 - +0.9 3,190,241 - -0.8 3,190,264 + +0.2 3,190,280 + -0.2 3,190,392 - +1.1 3,190,609 + Pf6N2E2_2697 0.27 -0.0 3,190,609 + Pf6N2E2_2697 0.27 +0.5 3,190,609 + Pf6N2E2_2697 0.27 +0.0 3,190,609 + Pf6N2E2_2697 0.27 +0.8 3,190,610 - Pf6N2E2_2697 0.27 -0.3 3,190,642 + Pf6N2E2_2697 0.30 +1.0 3,190,846 + Pf6N2E2_2697 0.53 +0.3 3,190,846 + Pf6N2E2_2697 0.53 -0.6 3,190,847 - Pf6N2E2_2697 0.53 -0.4 3,190,966 + Pf6N2E2_2697 0.66 +0.5 3,191,059 + Pf6N2E2_2697 0.76 +1.0 3,191,060 - Pf6N2E2_2697 0.76 +1.2 3,191,060 - Pf6N2E2_2697 0.76 -2.2 3,191,060 - Pf6N2E2_2697 0.76 +1.2 3,191,060 - Pf6N2E2_2697 0.76 +0.2 3,191,068 + Pf6N2E2_2697 0.77 +0.3 3,191,069 - Pf6N2E2_2697 0.77 +0.5 3,191,069 - Pf6N2E2_2697 0.77 +0.2 3,191,191 + -0.5 3,191,191 + +0.2 3,191,191 + +1.4 3,191,192 - +0.2 3,191,192 - +2.4 3,191,368 + -0.4 3,191,369 - +0.6 3,191,370 + +0.4 3,191,370 + -1.0 3,191,371 - +1.0 3,191,406 - -0.6 3,191,406 - +0.6 3,191,488 + Pf6N2E2_2698 0.11 +0.1 3,191,489 - Pf6N2E2_2698 0.11 -0.2 3,191,521 + Pf6N2E2_2698 0.15 -1.6 3,191,522 - Pf6N2E2_2698 0.15 +0.1 3,191,522 - Pf6N2E2_2698 0.15 -0.4 3,191,522 - Pf6N2E2_2698 0.15 +0.2 3,191,522 - Pf6N2E2_2698 0.15 -0.3 3,191,522 - Pf6N2E2_2698 0.15 +0.6 3,191,570 - Pf6N2E2_2698 0.20 +1.3 3,191,642 - Pf6N2E2_2698 0.29 -0.2 3,191,836 + Pf6N2E2_2698 0.51 -0.0 3,191,836 + Pf6N2E2_2698 0.51 -0.5 3,191,836 + Pf6N2E2_2698 0.51 -0.3 3,191,836 + Pf6N2E2_2698 0.51 +0.3 3,191,836 + Pf6N2E2_2698 0.51 +1.2 3,191,837 - Pf6N2E2_2698 0.51 +1.4 3,191,837 - Pf6N2E2_2698 0.51 -0.0 3,192,261 + +1.2 3,192,270 + -2.6 3,192,270 + +2.2 3,192,270 + +3.0 3,192,271 - -0.3 3,192,271 - -0.3 3,192,271 - -0.3 3,192,271 - +0.2
Or see this region's nucleotide sequence