Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1761 and Pf6N2E2_1762 are separated by 862 nucleotides Pf6N2E2_1762 and Pf6N2E2_1763 are separated by 51 nucleotides
Pf6N2E2_1761 - Transcriptional regulator, LysR family, at 2,079,717 to 2,080,400
_1761
Pf6N2E2_1762 - putative lipase, at 2,081,263 to 2,082,180
_1762
Pf6N2E2_1763 - Cyclohexanone monooxygenase (EC 1.14.13.22), at 2,082,232 to 2,084,154
_1763
Position (kb)
2081
2082
2083 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2080.479 kb on + strand at 2080.797 kb on - strand at 2080.918 kb on - strand at 2080.922 kb on + strand at 2080.953 kb on + strand at 2080.953 kb on + strand at 2080.954 kb on - strand at 2080.965 kb on - strand at 2080.965 kb on - strand at 2081.152 kb on + strand at 2081.292 kb on - strand at 2081.446 kb on + strand, within Pf6N2E2_1762 at 2081.507 kb on + strand, within Pf6N2E2_1762 at 2081.565 kb on + strand, within Pf6N2E2_1762 at 2081.630 kb on + strand, within Pf6N2E2_1762 at 2081.652 kb on - strand, within Pf6N2E2_1762 at 2081.833 kb on + strand, within Pf6N2E2_1762 at 2081.896 kb on + strand, within Pf6N2E2_1762 at 2081.897 kb on - strand, within Pf6N2E2_1762 at 2082.167 kb on + strand at 2082.168 kb on - strand at 2082.168 kb on - strand at 2082.184 kb on - strand at 2082.209 kb on + strand at 2082.210 kb on - strand at 2082.225 kb on + strand at 2082.226 kb on - strand at 2082.231 kb on + strand at 2082.231 kb on + strand at 2082.231 kb on + strand at 2082.232 kb on - strand at 2082.232 kb on - strand at 2082.232 kb on - strand at 2082.232 kb on - strand at 2082.232 kb on - strand at 2082.232 kb on - strand at 2082.241 kb on + strand at 2082.242 kb on - strand at 2082.242 kb on - strand at 2082.242 kb on - strand at 2082.562 kb on + strand, within Pf6N2E2_1763 at 2082.562 kb on + strand, within Pf6N2E2_1763 at 2082.662 kb on + strand, within Pf6N2E2_1763 at 2082.663 kb on - strand, within Pf6N2E2_1763 at 2082.740 kb on - strand, within Pf6N2E2_1763 at 2082.750 kb on + strand, within Pf6N2E2_1763 at 2082.821 kb on - strand, within Pf6N2E2_1763 at 2082.859 kb on + strand, within Pf6N2E2_1763 at 2082.860 kb on - strand, within Pf6N2E2_1763 at 2082.878 kb on + strand, within Pf6N2E2_1763 at 2082.879 kb on - strand, within Pf6N2E2_1763 at 2082.886 kb on + strand, within Pf6N2E2_1763 at 2082.887 kb on - strand, within Pf6N2E2_1763 at 2083.034 kb on + strand, within Pf6N2E2_1763 at 2083.035 kb on - strand, within Pf6N2E2_1763
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 2,080,479 + +0.5 2,080,797 - -0.8 2,080,918 - +0.8 2,080,922 + +1.6 2,080,953 + +0.1 2,080,953 + -0.5 2,080,954 - +0.8 2,080,965 - -2.3 2,080,965 - +2.7 2,081,152 + -0.6 2,081,292 - -0.3 2,081,446 + Pf6N2E2_1762 0.20 +0.3 2,081,507 + Pf6N2E2_1762 0.27 +0.1 2,081,565 + Pf6N2E2_1762 0.33 +0.1 2,081,630 + Pf6N2E2_1762 0.40 +1.7 2,081,652 - Pf6N2E2_1762 0.42 +0.1 2,081,833 + Pf6N2E2_1762 0.62 -0.5 2,081,896 + Pf6N2E2_1762 0.69 +1.2 2,081,897 - Pf6N2E2_1762 0.69 +0.5 2,082,167 + +0.1 2,082,168 - +0.1 2,082,168 - +1.9 2,082,184 - +1.0 2,082,209 + -0.4 2,082,210 - +0.9 2,082,225 + +1.0 2,082,226 - -0.8 2,082,231 + -1.8 2,082,231 + +2.3 2,082,231 + -0.3 2,082,232 - +0.2 2,082,232 - +1.1 2,082,232 - -2.1 2,082,232 - -0.2 2,082,232 - +0.2 2,082,232 - +0.1 2,082,241 + +0.4 2,082,242 - +0.3 2,082,242 - +0.1 2,082,242 - -1.1 2,082,562 + Pf6N2E2_1763 0.17 -0.5 2,082,562 + Pf6N2E2_1763 0.17 -0.3 2,082,662 + Pf6N2E2_1763 0.22 -1.9 2,082,663 - Pf6N2E2_1763 0.22 +0.3 2,082,740 - Pf6N2E2_1763 0.26 +1.9 2,082,750 + Pf6N2E2_1763 0.27 -0.3 2,082,821 - Pf6N2E2_1763 0.31 -0.1 2,082,859 + Pf6N2E2_1763 0.33 -0.1 2,082,860 - Pf6N2E2_1763 0.33 -0.4 2,082,878 + Pf6N2E2_1763 0.34 +0.3 2,082,879 - Pf6N2E2_1763 0.34 +1.1 2,082,886 + Pf6N2E2_1763 0.34 -0.1 2,082,887 - Pf6N2E2_1763 0.34 +0.5 2,083,034 + Pf6N2E2_1763 0.42 +0.0 2,083,035 - Pf6N2E2_1763 0.42 +1.1
Or see this region's nucleotide sequence