Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1622 and Pf6N2E2_1623 are separated by 157 nucleotides Pf6N2E2_1623 and Pf6N2E2_1624 are separated by 36 nucleotides Pf6N2E2_1624 and Pf6N2E2_1625 are separated by 203 nucleotides
Pf6N2E2_1622 - Methyl-accepting chemotaxis protein, at 1,896,899 to 1,898,869
_1622
Pf6N2E2_1623 - Siderophore biosynthesis diaminobutyrate--2-oxoglutarate aminotransferase (EC 2.6.1.76), at 1,899,027 to 1,900,457
_1623
Pf6N2E2_1624 - MbtH-like protein, at 1,900,494 to 1,900,709
_1624
Pf6N2E2_1625 - Ferric reductase (1.6.99.14), at 1,900,913 to 1,901,650
_1625
Position (kb)
1899
1900
1901 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1898.087 kb on + strand, within Pf6N2E2_1622 at 1898.088 kb on - strand, within Pf6N2E2_1622 at 1898.111 kb on + strand, within Pf6N2E2_1622 at 1898.112 kb on - strand, within Pf6N2E2_1622 at 1898.292 kb on + strand, within Pf6N2E2_1622 at 1898.292 kb on + strand, within Pf6N2E2_1622 at 1898.293 kb on - strand, within Pf6N2E2_1622 at 1898.345 kb on + strand, within Pf6N2E2_1622 at 1898.358 kb on + strand, within Pf6N2E2_1622 at 1898.359 kb on - strand, within Pf6N2E2_1622 at 1898.359 kb on - strand, within Pf6N2E2_1622 at 1898.359 kb on - strand, within Pf6N2E2_1622 at 1898.518 kb on - strand, within Pf6N2E2_1622 at 1899.036 kb on - strand at 1899.307 kb on - strand, within Pf6N2E2_1623 at 1899.489 kb on - strand, within Pf6N2E2_1623 at 1899.552 kb on + strand, within Pf6N2E2_1623 at 1899.553 kb on - strand, within Pf6N2E2_1623 at 1899.691 kb on - strand, within Pf6N2E2_1623 at 1900.276 kb on + strand, within Pf6N2E2_1623 at 1900.276 kb on + strand, within Pf6N2E2_1623 at 1900.277 kb on - strand, within Pf6N2E2_1623 at 1900.277 kb on - strand, within Pf6N2E2_1623 at 1900.434 kb on + strand at 1900.434 kb on + strand at 1900.435 kb on - strand at 1900.435 kb on - strand at 1900.473 kb on + strand at 1900.509 kb on + strand at 1900.509 kb on + strand at 1900.510 kb on - strand at 1900.510 kb on - strand at 1900.567 kb on + strand, within Pf6N2E2_1624 at 1900.567 kb on + strand, within Pf6N2E2_1624 at 1900.568 kb on - strand, within Pf6N2E2_1624 at 1900.568 kb on - strand, within Pf6N2E2_1624 at 1900.672 kb on - strand, within Pf6N2E2_1624 at 1900.672 kb on - strand, within Pf6N2E2_1624 at 1900.751 kb on - strand at 1900.855 kb on - strand at 1900.855 kb on - strand at 1900.856 kb on + strand at 1900.856 kb on + strand at 1900.857 kb on - strand at 1900.857 kb on - strand at 1901.001 kb on + strand, within Pf6N2E2_1625 at 1901.031 kb on - strand, within Pf6N2E2_1625 at 1901.291 kb on - strand, within Pf6N2E2_1625 at 1901.383 kb on + strand, within Pf6N2E2_1625 at 1901.384 kb on - strand, within Pf6N2E2_1625
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 1,898,087 + Pf6N2E2_1622 0.60 -0.5 1,898,088 - Pf6N2E2_1622 0.60 -1.2 1,898,111 + Pf6N2E2_1622 0.61 -0.7 1,898,112 - Pf6N2E2_1622 0.62 -0.2 1,898,292 + Pf6N2E2_1622 0.71 +0.6 1,898,292 + Pf6N2E2_1622 0.71 +0.4 1,898,293 - Pf6N2E2_1622 0.71 -0.1 1,898,345 + Pf6N2E2_1622 0.73 +0.6 1,898,358 + Pf6N2E2_1622 0.74 -0.7 1,898,359 - Pf6N2E2_1622 0.74 -0.4 1,898,359 - Pf6N2E2_1622 0.74 +2.1 1,898,359 - Pf6N2E2_1622 0.74 +1.4 1,898,518 - Pf6N2E2_1622 0.82 -0.7 1,899,036 - -1.6 1,899,307 - Pf6N2E2_1623 0.20 -0.2 1,899,489 - Pf6N2E2_1623 0.32 +1.1 1,899,552 + Pf6N2E2_1623 0.37 +2.6 1,899,553 - Pf6N2E2_1623 0.37 +1.5 1,899,691 - Pf6N2E2_1623 0.46 +0.3 1,900,276 + Pf6N2E2_1623 0.87 +0.6 1,900,276 + Pf6N2E2_1623 0.87 +1.2 1,900,277 - Pf6N2E2_1623 0.87 +1.0 1,900,277 - Pf6N2E2_1623 0.87 +1.1 1,900,434 + +1.3 1,900,434 + +0.7 1,900,435 - +1.4 1,900,435 - -0.5 1,900,473 + +2.1 1,900,509 + +0.9 1,900,509 + +1.7 1,900,510 - +0.6 1,900,510 - +1.4 1,900,567 + Pf6N2E2_1624 0.34 +0.9 1,900,567 + Pf6N2E2_1624 0.34 +0.4 1,900,568 - Pf6N2E2_1624 0.34 -0.4 1,900,568 - Pf6N2E2_1624 0.34 +0.2 1,900,672 - Pf6N2E2_1624 0.82 +0.7 1,900,672 - Pf6N2E2_1624 0.82 -0.1 1,900,751 - -0.9 1,900,855 - +0.8 1,900,855 - +0.6 1,900,856 + -1.4 1,900,856 + +0.8 1,900,857 - -0.6 1,900,857 - +2.0 1,901,001 + Pf6N2E2_1625 0.12 +0.2 1,901,031 - Pf6N2E2_1625 0.16 -2.3 1,901,291 - Pf6N2E2_1625 0.51 -0.8 1,901,383 + Pf6N2E2_1625 0.64 -0.7 1,901,384 - Pf6N2E2_1625 0.64 -2.1
Or see this region's nucleotide sequence