Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_124 and Pf6N2E2_125 overlap by 4 nucleotides Pf6N2E2_125 and Pf6N2E2_126 are separated by 376 nucleotides
Pf6N2E2_124 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 171,231 to 172,310
_124
Pf6N2E2_125 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 172,307 to 173,671
_125
Pf6N2E2_126 - FIG00960892: hypothetical protein, at 174,048 to 175,745
_126
Position (kb)
172
173
174 Strain fitness (log2 ratio)
-1
0
1
2 at 171.313 kb on + strand at 171.313 kb on + strand at 171.493 kb on + strand, within Pf6N2E2_124 at 171.493 kb on + strand, within Pf6N2E2_124 at 171.493 kb on + strand, within Pf6N2E2_124 at 171.532 kb on + strand, within Pf6N2E2_124 at 171.533 kb on - strand, within Pf6N2E2_124 at 171.649 kb on + strand, within Pf6N2E2_124 at 171.649 kb on + strand, within Pf6N2E2_124 at 171.811 kb on + strand, within Pf6N2E2_124 at 171.811 kb on + strand, within Pf6N2E2_124 at 171.812 kb on - strand, within Pf6N2E2_124 at 171.812 kb on - strand, within Pf6N2E2_124 at 171.812 kb on - strand, within Pf6N2E2_124 at 171.812 kb on - strand, within Pf6N2E2_124 at 171.812 kb on - strand, within Pf6N2E2_124 at 171.916 kb on + strand, within Pf6N2E2_124 at 171.916 kb on + strand, within Pf6N2E2_124 at 171.917 kb on - strand, within Pf6N2E2_124 at 171.937 kb on + strand, within Pf6N2E2_124 at 171.937 kb on + strand, within Pf6N2E2_124 at 171.937 kb on + strand, within Pf6N2E2_124 at 171.937 kb on + strand, within Pf6N2E2_124 at 171.937 kb on + strand, within Pf6N2E2_124 at 171.937 kb on + strand, within Pf6N2E2_124 at 171.981 kb on - strand, within Pf6N2E2_124 at 171.981 kb on - strand, within Pf6N2E2_124 at 171.981 kb on - strand, within Pf6N2E2_124 at 171.981 kb on - strand, within Pf6N2E2_124 at 171.981 kb on - strand, within Pf6N2E2_124 at 172.012 kb on + strand, within Pf6N2E2_124 at 172.013 kb on - strand, within Pf6N2E2_124 at 172.013 kb on - strand, within Pf6N2E2_124 at 172.013 kb on - strand, within Pf6N2E2_124 at 172.013 kb on - strand, within Pf6N2E2_124 at 172.013 kb on - strand, within Pf6N2E2_124 at 172.071 kb on + strand, within Pf6N2E2_124 at 172.202 kb on - strand, within Pf6N2E2_124 at 172.204 kb on + strand at 172.404 kb on + strand at 172.405 kb on - strand at 172.405 kb on - strand at 172.455 kb on - strand, within Pf6N2E2_125 at 172.569 kb on + strand, within Pf6N2E2_125 at 172.605 kb on + strand, within Pf6N2E2_125 at 172.768 kb on + strand, within Pf6N2E2_125 at 172.899 kb on + strand, within Pf6N2E2_125 at 173.106 kb on + strand, within Pf6N2E2_125 at 173.106 kb on + strand, within Pf6N2E2_125 at 173.228 kb on + strand, within Pf6N2E2_125 at 173.644 kb on - strand at 173.644 kb on - strand at 173.644 kb on - strand at 173.657 kb on + strand at 173.657 kb on + strand at 173.657 kb on + strand at 173.657 kb on + strand at 173.701 kb on + strand at 173.702 kb on - strand at 173.720 kb on + strand at 174.084 kb on + strand at 174.105 kb on + strand at 174.108 kb on + strand at 174.108 kb on + strand at 174.109 kb on - strand at 174.204 kb on + strand at 174.204 kb on + strand at 174.205 kb on - strand at 174.209 kb on - strand at 174.209 kb on - strand at 174.209 kb on - strand at 174.209 kb on - strand at 174.209 kb on - strand at 174.209 kb on - strand at 174.276 kb on + strand, within Pf6N2E2_126 at 174.276 kb on + strand, within Pf6N2E2_126 at 174.276 kb on + strand, within Pf6N2E2_126 at 174.276 kb on + strand, within Pf6N2E2_126 at 174.277 kb on - strand, within Pf6N2E2_126 at 174.277 kb on - strand, within Pf6N2E2_126 at 174.298 kb on - strand, within Pf6N2E2_126 at 174.298 kb on - strand, within Pf6N2E2_126 at 174.306 kb on + strand, within Pf6N2E2_126 at 174.306 kb on + strand, within Pf6N2E2_126 at 174.306 kb on + strand, within Pf6N2E2_126 at 174.306 kb on + strand, within Pf6N2E2_126 at 174.306 kb on + strand, within Pf6N2E2_126 at 174.307 kb on - strand, within Pf6N2E2_126 at 174.307 kb on - strand, within Pf6N2E2_126 at 174.307 kb on - strand, within Pf6N2E2_126 at 174.307 kb on - strand, within Pf6N2E2_126 at 174.308 kb on + strand, within Pf6N2E2_126 at 174.309 kb on - strand, within Pf6N2E2_126 at 174.330 kb on + strand, within Pf6N2E2_126 at 174.344 kb on + strand, within Pf6N2E2_126 at 174.360 kb on + strand, within Pf6N2E2_126 at 174.361 kb on - strand, within Pf6N2E2_126 at 174.412 kb on + strand, within Pf6N2E2_126 at 174.413 kb on - strand, within Pf6N2E2_126 at 174.460 kb on - strand, within Pf6N2E2_126 at 174.460 kb on - strand, within Pf6N2E2_126 at 174.555 kb on + strand, within Pf6N2E2_126 at 174.555 kb on + strand, within Pf6N2E2_126 at 174.555 kb on + strand, within Pf6N2E2_126 at 174.555 kb on + strand, within Pf6N2E2_126 at 174.556 kb on - strand, within Pf6N2E2_126 at 174.556 kb on - strand, within Pf6N2E2_126
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 171,313 + -0.8 171,313 + +0.3 171,493 + Pf6N2E2_124 0.24 -0.1 171,493 + Pf6N2E2_124 0.24 +0.9 171,493 + Pf6N2E2_124 0.24 +0.6 171,532 + Pf6N2E2_124 0.28 +0.7 171,533 - Pf6N2E2_124 0.28 -1.0 171,649 + Pf6N2E2_124 0.39 -0.3 171,649 + Pf6N2E2_124 0.39 +0.6 171,811 + Pf6N2E2_124 0.54 +0.3 171,811 + Pf6N2E2_124 0.54 -0.0 171,812 - Pf6N2E2_124 0.54 -0.2 171,812 - Pf6N2E2_124 0.54 -0.1 171,812 - Pf6N2E2_124 0.54 +0.2 171,812 - Pf6N2E2_124 0.54 +0.9 171,812 - Pf6N2E2_124 0.54 -0.7 171,916 + Pf6N2E2_124 0.63 -0.6 171,916 + Pf6N2E2_124 0.63 -1.4 171,917 - Pf6N2E2_124 0.64 +0.2 171,937 + Pf6N2E2_124 0.65 +0.6 171,937 + Pf6N2E2_124 0.65 +0.9 171,937 + Pf6N2E2_124 0.65 +0.8 171,937 + Pf6N2E2_124 0.65 +0.3 171,937 + Pf6N2E2_124 0.65 -0.4 171,937 + Pf6N2E2_124 0.65 -0.3 171,981 - Pf6N2E2_124 0.69 -1.1 171,981 - Pf6N2E2_124 0.69 +0.2 171,981 - Pf6N2E2_124 0.69 -0.9 171,981 - Pf6N2E2_124 0.69 -0.8 171,981 - Pf6N2E2_124 0.69 +1.2 172,012 + Pf6N2E2_124 0.72 -0.6 172,013 - Pf6N2E2_124 0.72 +0.0 172,013 - Pf6N2E2_124 0.72 +0.7 172,013 - Pf6N2E2_124 0.72 -0.1 172,013 - Pf6N2E2_124 0.72 +1.5 172,013 - Pf6N2E2_124 0.72 +0.7 172,071 + Pf6N2E2_124 0.78 -0.3 172,202 - Pf6N2E2_124 0.90 -0.8 172,204 + -0.0 172,404 + +0.5 172,405 - -0.9 172,405 - +0.6 172,455 - Pf6N2E2_125 0.11 +1.1 172,569 + Pf6N2E2_125 0.19 +0.6 172,605 + Pf6N2E2_125 0.22 +0.2 172,768 + Pf6N2E2_125 0.34 +0.2 172,899 + Pf6N2E2_125 0.43 +1.9 173,106 + Pf6N2E2_125 0.59 +0.7 173,106 + Pf6N2E2_125 0.59 -1.1 173,228 + Pf6N2E2_125 0.67 +1.4 173,644 - +0.5 173,644 - +1.0 173,644 - +0.7 173,657 + +0.3 173,657 + +0.4 173,657 + -0.8 173,657 + +1.3 173,701 + +1.1 173,702 - -1.3 173,720 + +0.2 174,084 + +0.6 174,105 + +0.9 174,108 + +0.4 174,108 + -0.1 174,109 - +0.2 174,204 + -0.0 174,204 + -0.6 174,205 - -0.4 174,209 - +0.7 174,209 - -0.1 174,209 - -1.0 174,209 - +0.2 174,209 - -0.9 174,209 - +0.4 174,276 + Pf6N2E2_126 0.13 -0.3 174,276 + Pf6N2E2_126 0.13 -0.4 174,276 + Pf6N2E2_126 0.13 +0.0 174,276 + Pf6N2E2_126 0.13 -0.6 174,277 - Pf6N2E2_126 0.13 +1.0 174,277 - Pf6N2E2_126 0.13 +0.2 174,298 - Pf6N2E2_126 0.15 +0.1 174,298 - Pf6N2E2_126 0.15 +0.3 174,306 + Pf6N2E2_126 0.15 +0.3 174,306 + Pf6N2E2_126 0.15 -0.3 174,306 + Pf6N2E2_126 0.15 -0.9 174,306 + Pf6N2E2_126 0.15 +1.9 174,306 + Pf6N2E2_126 0.15 -0.3 174,307 - Pf6N2E2_126 0.15 +0.6 174,307 - Pf6N2E2_126 0.15 -1.5 174,307 - Pf6N2E2_126 0.15 -0.8 174,307 - Pf6N2E2_126 0.15 -0.1 174,308 + Pf6N2E2_126 0.15 +0.2 174,309 - Pf6N2E2_126 0.15 -1.0 174,330 + Pf6N2E2_126 0.17 +0.6 174,344 + Pf6N2E2_126 0.17 +0.8 174,360 + Pf6N2E2_126 0.18 -0.8 174,361 - Pf6N2E2_126 0.18 +1.3 174,412 + Pf6N2E2_126 0.21 +0.1 174,413 - Pf6N2E2_126 0.21 +1.4 174,460 - Pf6N2E2_126 0.24 +1.9 174,460 - Pf6N2E2_126 0.24 -1.1 174,555 + Pf6N2E2_126 0.30 +0.3 174,555 + Pf6N2E2_126 0.30 -1.6 174,555 + Pf6N2E2_126 0.30 -0.7 174,555 + Pf6N2E2_126 0.30 -0.3 174,556 - Pf6N2E2_126 0.30 -0.1 174,556 - Pf6N2E2_126 0.30 -1.4
Or see this region's nucleotide sequence