Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00800

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00790 and BWI76_RS00795 are separated by 39 nucleotidesBWI76_RS00795 and BWI76_RS00800 are separated by 91 nucleotidesBWI76_RS00800 and BWI76_RS00805 are separated by 166 nucleotides BWI76_RS00790: BWI76_RS00790 - bifunctional aspartokinase II/homoserine dehydrogenase II, at 160,976 to 163,408 _RS00790 BWI76_RS00795: BWI76_RS00795 - carboxymuconolactone decarboxylase, at 163,448 to 163,819 _RS00795 BWI76_RS00800: BWI76_RS00800 - LysR family transcriptional regulator, at 163,911 to 164,816 _RS00800 BWI76_RS00805: BWI76_RS00805 - 5,10-methylenetetrahydrofolate reductase, at 164,983 to 165,870 _RS00805 Position (kb) 163 164 165Strain fitness (log2 ratio) -2 -1 0 1 2at 162.911 kb on - strand, within BWI76_RS00790at 163.095 kb on + strand, within BWI76_RS00790at 163.183 kb on + strandat 163.191 kb on - strandat 163.379 kb on + strandat 163.613 kb on - strand, within BWI76_RS00795at 163.650 kb on - strand, within BWI76_RS00795at 163.740 kb on - strand, within BWI76_RS00795at 163.778 kb on + strand, within BWI76_RS00795at 163.799 kb on + strandat 163.822 kb on - strandat 163.839 kb on - strandat 163.866 kb on + strandat 163.889 kb on - strandat 164.060 kb on + strandat 164.154 kb on + strand, within BWI76_RS00800at 164.233 kb on + strand, within BWI76_RS00800at 164.241 kb on - strand, within BWI76_RS00800at 164.241 kb on - strand, within BWI76_RS00800at 164.464 kb on + strand, within BWI76_RS00800at 164.734 kb on - strandat 164.739 kb on - strandat 164.745 kb on - strandat 164.791 kb on - strandat 164.988 kb on - strandat 165.056 kb on - strandat 165.139 kb on - strand, within BWI76_RS00805at 165.252 kb on - strand, within BWI76_RS00805at 165.364 kb on - strand, within BWI76_RS00805at 165.492 kb on + strand, within BWI76_RS00805at 165.803 kb on + strandat 165.808 kb on + strandat 165.811 kb on - strandat 165.816 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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162,911 - BWI76_RS00790 0.80 +0.3
163,095 + BWI76_RS00790 0.87 -0.1
163,183 + +0.5
163,191 - +0.3
163,379 + -0.5
163,613 - BWI76_RS00795 0.44 -0.1
163,650 - BWI76_RS00795 0.54 +1.1
163,740 - BWI76_RS00795 0.78 -0.2
163,778 + BWI76_RS00795 0.89 +0.8
163,799 + +0.4
163,822 - -0.9
163,839 - -0.3
163,866 + -0.3
163,889 - -0.5
164,060 + +2.3
164,154 + BWI76_RS00800 0.27 -2.7
164,233 + BWI76_RS00800 0.36 -1.7
164,241 - BWI76_RS00800 0.36 -0.3
164,241 - BWI76_RS00800 0.36 -0.5
164,464 + BWI76_RS00800 0.61 -0.2
164,734 - +0.6
164,739 - +0.7
164,745 - +0.5
164,791 - -1.1
164,988 - +1.3
165,056 - -0.3
165,139 - BWI76_RS00805 0.18 -0.4
165,252 - BWI76_RS00805 0.30 +0.1
165,364 - BWI76_RS00805 0.43 -0.2
165,492 + BWI76_RS00805 0.57 -0.4
165,803 + +0.1
165,808 + +0.5
165,811 - +0.3
165,816 - +2.0

Or see this region's nucleotide sequence