Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS37460 and RR42_RS37465 are separated by 463 nucleotides RR42_RS37465 and RR42_RS37470 are separated by 186 nucleotides
RR42_RS37460: RR42_RS37460 - MFS transporter, at 3,882,654 to 3,883,907
_RS37460
RR42_RS37465: RR42_RS37465 - LysR family transcriptional regulator, at 3,884,371 to 3,885,282
_RS37465
RR42_RS37470: RR42_RS37470 - permease, at 3,885,469 to 3,886,239
_RS37470
Position (kb)
3884
3885
3886 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3883.393 kb on + strand, within RR42_RS37460 at 3883.768 kb on + strand, within RR42_RS37460 at 3883.768 kb on + strand, within RR42_RS37460 at 3883.768 kb on + strand, within RR42_RS37460 at 3883.782 kb on + strand at 3883.782 kb on + strand at 3883.794 kb on - strand at 3883.957 kb on + strand at 3884.031 kb on + strand at 3884.032 kb on - strand at 3884.032 kb on - strand at 3884.032 kb on - strand at 3884.032 kb on - strand at 3884.356 kb on - strand at 3884.356 kb on - strand at 3884.473 kb on - strand, within RR42_RS37465 at 3884.473 kb on - strand, within RR42_RS37465 at 3884.621 kb on + strand, within RR42_RS37465 at 3884.715 kb on + strand, within RR42_RS37465 at 3884.715 kb on + strand, within RR42_RS37465 at 3884.715 kb on + strand, within RR42_RS37465 at 3884.716 kb on - strand, within RR42_RS37465 at 3884.762 kb on - strand, within RR42_RS37465 at 3884.762 kb on - strand, within RR42_RS37465 at 3884.787 kb on - strand, within RR42_RS37465 at 3884.787 kb on - strand, within RR42_RS37465 at 3884.787 kb on - strand, within RR42_RS37465 at 3884.787 kb on - strand, within RR42_RS37465 at 3884.914 kb on + strand, within RR42_RS37465 at 3884.915 kb on - strand, within RR42_RS37465 at 3884.915 kb on - strand, within RR42_RS37465 at 3884.915 kb on - strand, within RR42_RS37465 at 3884.936 kb on + strand, within RR42_RS37465 at 3885.255 kb on - strand at 3885.413 kb on + strand at 3885.414 kb on - strand at 3885.414 kb on - strand at 3885.439 kb on + strand at 3885.439 kb on + strand at 3885.439 kb on + strand at 3885.439 kb on + strand at 3885.517 kb on + strand at 3885.550 kb on + strand, within RR42_RS37470 at 3885.551 kb on - strand, within RR42_RS37470 at 3885.551 kb on - strand, within RR42_RS37470 at 3885.611 kb on - strand, within RR42_RS37470 at 3885.641 kb on + strand, within RR42_RS37470 at 3885.813 kb on - strand, within RR42_RS37470 at 3885.813 kb on - strand, within RR42_RS37470 at 3885.944 kb on - strand, within RR42_RS37470 at 3885.944 kb on - strand, within RR42_RS37470 at 3885.944 kb on - strand, within RR42_RS37470 at 3885.952 kb on + strand, within RR42_RS37470 at 3885.952 kb on + strand, within RR42_RS37470 at 3885.952 kb on + strand, within RR42_RS37470 at 3885.952 kb on + strand, within RR42_RS37470 at 3885.952 kb on + strand, within RR42_RS37470 at 3885.953 kb on - strand, within RR42_RS37470 at 3885.953 kb on - strand, within RR42_RS37470 at 3886.017 kb on + strand, within RR42_RS37470 at 3886.037 kb on + strand, within RR42_RS37470 at 3886.037 kb on + strand, within RR42_RS37470 at 3886.038 kb on - strand, within RR42_RS37470 at 3886.038 kb on - strand, within RR42_RS37470 at 3886.038 kb on - strand, within RR42_RS37470 at 3886.038 kb on - strand, within RR42_RS37470 at 3886.038 kb on - strand, within RR42_RS37470 at 3886.057 kb on + strand, within RR42_RS37470 at 3886.058 kb on - strand, within RR42_RS37470 at 3886.219 kb on + strand at 3886.219 kb on + strand at 3886.219 kb on + strand at 3886.220 kb on - strand at 3886.238 kb on - strand at 3886.238 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 3,883,393 + RR42_RS37460 0.59 +0.2 3,883,768 + RR42_RS37460 0.89 -0.7 3,883,768 + RR42_RS37460 0.89 -0.7 3,883,768 + RR42_RS37460 0.89 -1.5 3,883,782 + +0.4 3,883,782 + -1.5 3,883,794 - -0.9 3,883,957 + +0.4 3,884,031 + +0.1 3,884,032 - -0.3 3,884,032 - -0.2 3,884,032 - -0.9 3,884,032 - +1.0 3,884,356 - -0.5 3,884,356 - +1.3 3,884,473 - RR42_RS37465 0.11 -1.0 3,884,473 - RR42_RS37465 0.11 +1.9 3,884,621 + RR42_RS37465 0.27 +0.3 3,884,715 + RR42_RS37465 0.38 -0.9 3,884,715 + RR42_RS37465 0.38 -0.4 3,884,715 + RR42_RS37465 0.38 +1.0 3,884,716 - RR42_RS37465 0.38 +0.4 3,884,762 - RR42_RS37465 0.43 -3.6 3,884,762 - RR42_RS37465 0.43 +1.1 3,884,787 - RR42_RS37465 0.46 +0.6 3,884,787 - RR42_RS37465 0.46 -0.2 3,884,787 - RR42_RS37465 0.46 +0.6 3,884,787 - RR42_RS37465 0.46 -0.6 3,884,914 + RR42_RS37465 0.60 +0.2 3,884,915 - RR42_RS37465 0.60 +0.7 3,884,915 - RR42_RS37465 0.60 -0.4 3,884,915 - RR42_RS37465 0.60 -3.0 3,884,936 + RR42_RS37465 0.62 +0.1 3,885,255 - -1.4 3,885,413 + +0.1 3,885,414 - -0.4 3,885,414 - -0.3 3,885,439 + -0.2 3,885,439 + -3.1 3,885,439 + +0.2 3,885,439 + +0.4 3,885,517 + +1.2 3,885,550 + RR42_RS37470 0.11 -0.4 3,885,551 - RR42_RS37470 0.11 +0.4 3,885,551 - RR42_RS37470 0.11 -1.1 3,885,611 - RR42_RS37470 0.18 -0.8 3,885,641 + RR42_RS37470 0.22 -0.0 3,885,813 - RR42_RS37470 0.45 -0.1 3,885,813 - RR42_RS37470 0.45 +0.5 3,885,944 - RR42_RS37470 0.62 -0.9 3,885,944 - RR42_RS37470 0.62 +0.6 3,885,944 - RR42_RS37470 0.62 -0.7 3,885,952 + RR42_RS37470 0.63 +0.1 3,885,952 + RR42_RS37470 0.63 -0.5 3,885,952 + RR42_RS37470 0.63 -0.1 3,885,952 + RR42_RS37470 0.63 -0.1 3,885,952 + RR42_RS37470 0.63 -0.2 3,885,953 - RR42_RS37470 0.63 -0.3 3,885,953 - RR42_RS37470 0.63 -0.3 3,886,017 + RR42_RS37470 0.71 -0.1 3,886,037 + RR42_RS37470 0.74 -0.4 3,886,037 + RR42_RS37470 0.74 -0.2 3,886,038 - RR42_RS37470 0.74 +0.4 3,886,038 - RR42_RS37470 0.74 +0.8 3,886,038 - RR42_RS37470 0.74 +0.0 3,886,038 - RR42_RS37470 0.74 -0.9 3,886,038 - RR42_RS37470 0.74 +0.3 3,886,057 + RR42_RS37470 0.76 +0.7 3,886,058 - RR42_RS37470 0.76 -0.7 3,886,219 + +0.4 3,886,219 + +0.4 3,886,219 + -0.7 3,886,220 - +0.3 3,886,238 - +0.5 3,886,238 - -0.7
Or see this region's nucleotide sequence