Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35630 and RR42_RS35635 are separated by 7 nucleotides RR42_RS35635 and RR42_RS35640 are separated by 13 nucleotides RR42_RS35640 and RR42_RS35645 overlap by 10 nucleotides
RR42_RS35630: RR42_RS35630 - ArsR family transcriptional regulator, at 3,447,660 to 3,448,313
_RS35630
RR42_RS35635: RR42_RS35635 - hypothetical protein, at 3,448,321 to 3,448,671
_RS35635
RR42_RS35640: RR42_RS35640 - MBL fold metallo-hydrolase, at 3,448,685 to 3,449,440
_RS35640
RR42_RS35645: RR42_RS35645 - MFS transporter, at 3,449,431 to 3,450,732
_RS35645
Position (kb)
3448
3449
3450 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3447.685 kb on - strand at 3447.685 kb on - strand at 3447.685 kb on - strand at 3447.694 kb on + strand at 3447.713 kb on + strand at 3447.713 kb on + strand at 3447.850 kb on + strand, within RR42_RS35630 at 3447.851 kb on - strand, within RR42_RS35630 at 3447.851 kb on - strand, within RR42_RS35630 at 3447.876 kb on + strand, within RR42_RS35630 at 3447.876 kb on + strand, within RR42_RS35630 at 3447.877 kb on - strand, within RR42_RS35630 at 3447.877 kb on - strand, within RR42_RS35630 at 3447.877 kb on - strand, within RR42_RS35630 at 3447.877 kb on - strand, within RR42_RS35630 at 3447.877 kb on - strand, within RR42_RS35630 at 3447.917 kb on - strand, within RR42_RS35630 at 3448.054 kb on + strand, within RR42_RS35630 at 3448.055 kb on - strand, within RR42_RS35630 at 3448.055 kb on - strand, within RR42_RS35630 at 3448.057 kb on + strand, within RR42_RS35630 at 3448.136 kb on + strand, within RR42_RS35630 at 3448.136 kb on + strand, within RR42_RS35630 at 3448.136 kb on + strand, within RR42_RS35630 at 3448.183 kb on - strand, within RR42_RS35630 at 3448.183 kb on - strand, within RR42_RS35630 at 3448.311 kb on + strand at 3448.311 kb on + strand at 3448.311 kb on + strand at 3448.311 kb on + strand at 3448.311 kb on + strand at 3448.312 kb on - strand at 3448.312 kb on - strand at 3448.354 kb on + strand at 3448.368 kb on + strand, within RR42_RS35635 at 3448.368 kb on + strand, within RR42_RS35635 at 3448.368 kb on + strand, within RR42_RS35635 at 3448.369 kb on - strand, within RR42_RS35635 at 3448.372 kb on + strand, within RR42_RS35635 at 3448.372 kb on + strand, within RR42_RS35635 at 3448.373 kb on - strand, within RR42_RS35635 at 3448.400 kb on + strand, within RR42_RS35635 at 3448.400 kb on + strand, within RR42_RS35635 at 3448.492 kb on + strand, within RR42_RS35635 at 3448.492 kb on + strand, within RR42_RS35635 at 3448.493 kb on - strand, within RR42_RS35635 at 3448.493 kb on - strand, within RR42_RS35635 at 3448.493 kb on - strand, within RR42_RS35635 at 3448.493 kb on - strand, within RR42_RS35635 at 3448.594 kb on - strand, within RR42_RS35635 at 3448.594 kb on - strand, within RR42_RS35635 at 3448.594 kb on - strand, within RR42_RS35635 at 3448.594 kb on - strand, within RR42_RS35635 at 3448.868 kb on + strand, within RR42_RS35640 at 3448.868 kb on + strand, within RR42_RS35640 at 3448.910 kb on + strand, within RR42_RS35640 at 3448.911 kb on - strand, within RR42_RS35640 at 3448.913 kb on + strand, within RR42_RS35640 at 3448.914 kb on - strand, within RR42_RS35640 at 3449.026 kb on + strand, within RR42_RS35640 at 3449.027 kb on - strand, within RR42_RS35640 at 3449.027 kb on - strand, within RR42_RS35640 at 3449.027 kb on - strand, within RR42_RS35640 at 3449.052 kb on + strand, within RR42_RS35640 at 3449.052 kb on + strand, within RR42_RS35640 at 3449.053 kb on - strand, within RR42_RS35640 at 3449.053 kb on - strand, within RR42_RS35640 at 3449.053 kb on - strand, within RR42_RS35640 at 3449.172 kb on - strand, within RR42_RS35640 at 3449.316 kb on + strand, within RR42_RS35640 at 3449.317 kb on - strand, within RR42_RS35640 at 3449.317 kb on - strand, within RR42_RS35640 at 3449.317 kb on - strand, within RR42_RS35640 at 3449.532 kb on + strand at 3449.533 kb on - strand at 3449.533 kb on - strand at 3449.549 kb on + strand at 3449.676 kb on + strand, within RR42_RS35645 at 3449.676 kb on + strand, within RR42_RS35645 at 3449.677 kb on - strand, within RR42_RS35645 at 3449.741 kb on - strand, within RR42_RS35645 at 3449.772 kb on - strand, within RR42_RS35645 at 3449.864 kb on + strand, within RR42_RS35645 at 3449.865 kb on - strand, within RR42_RS35645 at 3449.893 kb on + strand, within RR42_RS35645 at 3449.966 kb on + strand, within RR42_RS35645 at 3449.967 kb on - strand, within RR42_RS35645 at 3449.967 kb on - strand, within RR42_RS35645 at 3450.014 kb on + strand, within RR42_RS35645 at 3450.089 kb on + strand, within RR42_RS35645 at 3450.090 kb on - strand, within RR42_RS35645 at 3450.244 kb on + strand, within RR42_RS35645 at 3450.244 kb on + strand, within RR42_RS35645 at 3450.244 kb on + strand, within RR42_RS35645 at 3450.299 kb on - strand, within RR42_RS35645 at 3450.414 kb on - strand, within RR42_RS35645
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 3,447,685 - +0.0 3,447,685 - +0.7 3,447,685 - +0.2 3,447,694 + +0.6 3,447,713 + +1.2 3,447,713 + +0.9 3,447,850 + RR42_RS35630 0.29 +1.5 3,447,851 - RR42_RS35630 0.29 -0.4 3,447,851 - RR42_RS35630 0.29 -1.2 3,447,876 + RR42_RS35630 0.33 +0.6 3,447,876 + RR42_RS35630 0.33 +1.2 3,447,877 - RR42_RS35630 0.33 -1.0 3,447,877 - RR42_RS35630 0.33 -0.8 3,447,877 - RR42_RS35630 0.33 +0.0 3,447,877 - RR42_RS35630 0.33 +1.3 3,447,877 - RR42_RS35630 0.33 -0.9 3,447,917 - RR42_RS35630 0.39 -0.7 3,448,054 + RR42_RS35630 0.60 +1.4 3,448,055 - RR42_RS35630 0.60 +0.2 3,448,055 - RR42_RS35630 0.60 +0.1 3,448,057 + RR42_RS35630 0.61 -1.7 3,448,136 + RR42_RS35630 0.73 +1.7 3,448,136 + RR42_RS35630 0.73 +1.4 3,448,136 + RR42_RS35630 0.73 +1.4 3,448,183 - RR42_RS35630 0.80 -0.2 3,448,183 - RR42_RS35630 0.80 +0.2 3,448,311 + +2.4 3,448,311 + +1.5 3,448,311 + +1.6 3,448,311 + +1.6 3,448,311 + +0.8 3,448,312 - -0.3 3,448,312 - +1.1 3,448,354 + +1.0 3,448,368 + RR42_RS35635 0.13 +0.9 3,448,368 + RR42_RS35635 0.13 +0.1 3,448,368 + RR42_RS35635 0.13 +0.7 3,448,369 - RR42_RS35635 0.14 +0.2 3,448,372 + RR42_RS35635 0.15 +0.7 3,448,372 + RR42_RS35635 0.15 +1.2 3,448,373 - RR42_RS35635 0.15 +0.4 3,448,400 + RR42_RS35635 0.23 +1.2 3,448,400 + RR42_RS35635 0.23 +0.9 3,448,492 + RR42_RS35635 0.49 +1.3 3,448,492 + RR42_RS35635 0.49 +1.1 3,448,493 - RR42_RS35635 0.49 -0.2 3,448,493 - RR42_RS35635 0.49 -0.6 3,448,493 - RR42_RS35635 0.49 +0.1 3,448,493 - RR42_RS35635 0.49 -0.1 3,448,594 - RR42_RS35635 0.78 +0.5 3,448,594 - RR42_RS35635 0.78 -0.1 3,448,594 - RR42_RS35635 0.78 -1.3 3,448,594 - RR42_RS35635 0.78 +0.6 3,448,868 + RR42_RS35640 0.24 -0.2 3,448,868 + RR42_RS35640 0.24 +0.5 3,448,910 + RR42_RS35640 0.30 +1.6 3,448,911 - RR42_RS35640 0.30 -0.4 3,448,913 + RR42_RS35640 0.30 +0.0 3,448,914 - RR42_RS35640 0.30 -0.1 3,449,026 + RR42_RS35640 0.45 +0.2 3,449,027 - RR42_RS35640 0.45 -1.0 3,449,027 - RR42_RS35640 0.45 -0.5 3,449,027 - RR42_RS35640 0.45 -0.9 3,449,052 + RR42_RS35640 0.49 +0.3 3,449,052 + RR42_RS35640 0.49 -0.0 3,449,053 - RR42_RS35640 0.49 -1.3 3,449,053 - RR42_RS35640 0.49 +0.4 3,449,053 - RR42_RS35640 0.49 -0.9 3,449,172 - RR42_RS35640 0.64 -0.1 3,449,316 + RR42_RS35640 0.83 +0.5 3,449,317 - RR42_RS35640 0.84 -0.2 3,449,317 - RR42_RS35640 0.84 -2.3 3,449,317 - RR42_RS35640 0.84 +0.6 3,449,532 + +1.3 3,449,533 - -2.2 3,449,533 - -0.9 3,449,549 + +1.0 3,449,676 + RR42_RS35645 0.19 +1.1 3,449,676 + RR42_RS35645 0.19 +1.3 3,449,677 - RR42_RS35645 0.19 -2.0 3,449,741 - RR42_RS35645 0.24 +0.1 3,449,772 - RR42_RS35645 0.26 +0.5 3,449,864 + RR42_RS35645 0.33 +1.5 3,449,865 - RR42_RS35645 0.33 +0.0 3,449,893 + RR42_RS35645 0.35 +1.8 3,449,966 + RR42_RS35645 0.41 +1.8 3,449,967 - RR42_RS35645 0.41 +0.2 3,449,967 - RR42_RS35645 0.41 -0.4 3,450,014 + RR42_RS35645 0.45 +1.7 3,450,089 + RR42_RS35645 0.51 +1.6 3,450,090 - RR42_RS35645 0.51 -0.3 3,450,244 + RR42_RS35645 0.62 +0.6 3,450,244 + RR42_RS35645 0.62 +0.4 3,450,244 + RR42_RS35645 0.62 +1.9 3,450,299 - RR42_RS35645 0.67 +0.1 3,450,414 - RR42_RS35645 0.75 -0.2
Or see this region's nucleotide sequence