Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS34940

Experiment: R2A with nitrate 150 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS34930 and RR42_RS34935 are separated by 70 nucleotidesRR42_RS34935 and RR42_RS34940 are separated by 125 nucleotidesRR42_RS34940 and RR42_RS34945 are separated by 33 nucleotidesRR42_RS34945 and RR42_RS34950 overlap by 4 nucleotides RR42_RS34930: RR42_RS34930 - succinate-semialdehyde dehydrogenase, at 3,288,056 to 3,289,261 _RS34930 RR42_RS34935: RR42_RS34935 - cytochrome C oxidase subunit IV, at 3,289,332 to 3,289,616 _RS34935 RR42_RS34940: RR42_RS34940 - bb3-type cytochrome oxidase subunit IV, at 3,289,742 to 3,290,425 _RS34940 RR42_RS34945: RR42_RS34945 - bb3-type cytochrome oxidase subunit III, at 3,290,459 to 3,291,097 _RS34945 RR42_RS34950: RR42_RS34950 - cytochrome C oxidase, at 3,291,094 to 3,292,851 _RS34950 Position (kb) 3289 3290 3291Strain fitness (log2 ratio) -2 -1 0 1 2at 3289.023 kb on + strand, within RR42_RS34930at 3289.086 kb on + strand, within RR42_RS34930at 3289.086 kb on + strand, within RR42_RS34930at 3289.087 kb on - strand, within RR42_RS34930at 3289.087 kb on - strand, within RR42_RS34930at 3289.087 kb on - strand, within RR42_RS34930at 3289.087 kb on - strand, within RR42_RS34930at 3289.087 kb on - strand, within RR42_RS34930at 3289.087 kb on - strand, within RR42_RS34930at 3289.228 kb on + strandat 3289.228 kb on + strandat 3289.229 kb on - strandat 3289.230 kb on + strandat 3289.230 kb on + strandat 3289.231 kb on - strandat 3289.231 kb on - strandat 3289.231 kb on - strandat 3289.297 kb on - strandat 3289.531 kb on + strand, within RR42_RS34935at 3289.549 kb on + strand, within RR42_RS34935at 3289.549 kb on + strand, within RR42_RS34935at 3289.549 kb on + strand, within RR42_RS34935at 3289.606 kb on + strandat 3289.606 kb on + strandat 3289.769 kb on - strandat 3290.259 kb on + strand, within RR42_RS34940at 3290.259 kb on + strand, within RR42_RS34940at 3290.664 kb on + strand, within RR42_RS34945at 3290.665 kb on - strand, within RR42_RS34945at 3290.940 kb on + strand, within RR42_RS34945at 3291.197 kb on + strandat 3291.197 kb on + strandat 3291.197 kb on + strandat 3291.198 kb on - strandat 3291.198 kb on - strandat 3291.203 kb on + strandat 3291.203 kb on + strandat 3291.204 kb on - strandat 3291.378 kb on + strand, within RR42_RS34950at 3291.396 kb on + strand, within RR42_RS34950at 3291.396 kb on + strand, within RR42_RS34950at 3291.397 kb on - strand, within RR42_RS34950at 3291.397 kb on - strand, within RR42_RS34950at 3291.398 kb on + strand, within RR42_RS34950

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM
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3,289,023 + RR42_RS34930 0.80 -0.5
3,289,086 + RR42_RS34930 0.85 -2.3
3,289,086 + RR42_RS34930 0.85 +1.1
3,289,087 - RR42_RS34930 0.85 +0.7
3,289,087 - RR42_RS34930 0.85 +0.0
3,289,087 - RR42_RS34930 0.85 +0.6
3,289,087 - RR42_RS34930 0.85 -2.1
3,289,087 - RR42_RS34930 0.85 +0.3
3,289,087 - RR42_RS34930 0.85 -0.5
3,289,228 + -0.7
3,289,228 + -0.9
3,289,229 - +0.2
3,289,230 + +2.2
3,289,230 + -1.4
3,289,231 - +0.5
3,289,231 - -0.1
3,289,231 - +0.2
3,289,297 - -0.7
3,289,531 + RR42_RS34935 0.70 +0.4
3,289,549 + RR42_RS34935 0.76 -0.4
3,289,549 + RR42_RS34935 0.76 -0.6
3,289,549 + RR42_RS34935 0.76 +0.3
3,289,606 + +1.3
3,289,606 + -0.6
3,289,769 - -0.1
3,290,259 + RR42_RS34940 0.76 -0.4
3,290,259 + RR42_RS34940 0.76 -0.3
3,290,664 + RR42_RS34945 0.32 +0.8
3,290,665 - RR42_RS34945 0.32 -0.2
3,290,940 + RR42_RS34945 0.75 +0.2
3,291,197 + +0.4
3,291,197 + +0.3
3,291,197 + +0.1
3,291,198 - -0.8
3,291,198 - +0.8
3,291,203 + +0.2
3,291,203 + -0.7
3,291,204 - -1.0
3,291,378 + RR42_RS34950 0.16 -1.4
3,291,396 + RR42_RS34950 0.17 +0.0
3,291,396 + RR42_RS34950 0.17 +0.8
3,291,397 - RR42_RS34950 0.17 +0.3
3,291,397 - RR42_RS34950 0.17 +0.4
3,291,398 + RR42_RS34950 0.17 -0.2

Or see this region's nucleotide sequence