Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34930 and RR42_RS34935 are separated by 70 nucleotides RR42_RS34935 and RR42_RS34940 are separated by 125 nucleotides RR42_RS34940 and RR42_RS34945 are separated by 33 nucleotides RR42_RS34945 and RR42_RS34950 overlap by 4 nucleotides
RR42_RS34930: RR42_RS34930 - succinate-semialdehyde dehydrogenase, at 3,288,056 to 3,289,261
_RS34930
RR42_RS34935: RR42_RS34935 - cytochrome C oxidase subunit IV, at 3,289,332 to 3,289,616
_RS34935
RR42_RS34940: RR42_RS34940 - bb3-type cytochrome oxidase subunit IV, at 3,289,742 to 3,290,425
_RS34940
RR42_RS34945: RR42_RS34945 - bb3-type cytochrome oxidase subunit III, at 3,290,459 to 3,291,097
_RS34945
RR42_RS34950: RR42_RS34950 - cytochrome C oxidase, at 3,291,094 to 3,292,851
_RS34950
Position (kb)
3289
3290
3291 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3289.023 kb on + strand, within RR42_RS34930 at 3289.086 kb on + strand, within RR42_RS34930 at 3289.086 kb on + strand, within RR42_RS34930 at 3289.087 kb on - strand, within RR42_RS34930 at 3289.087 kb on - strand, within RR42_RS34930 at 3289.087 kb on - strand, within RR42_RS34930 at 3289.087 kb on - strand, within RR42_RS34930 at 3289.087 kb on - strand, within RR42_RS34930 at 3289.087 kb on - strand, within RR42_RS34930 at 3289.228 kb on + strand at 3289.228 kb on + strand at 3289.229 kb on - strand at 3289.230 kb on + strand at 3289.230 kb on + strand at 3289.231 kb on - strand at 3289.231 kb on - strand at 3289.231 kb on - strand at 3289.297 kb on - strand at 3289.531 kb on + strand, within RR42_RS34935 at 3289.549 kb on + strand, within RR42_RS34935 at 3289.549 kb on + strand, within RR42_RS34935 at 3289.549 kb on + strand, within RR42_RS34935 at 3289.606 kb on + strand at 3289.606 kb on + strand at 3289.769 kb on - strand at 3290.259 kb on + strand, within RR42_RS34940 at 3290.259 kb on + strand, within RR42_RS34940 at 3290.664 kb on + strand, within RR42_RS34945 at 3290.665 kb on - strand, within RR42_RS34945 at 3290.940 kb on + strand, within RR42_RS34945 at 3291.197 kb on + strand at 3291.197 kb on + strand at 3291.197 kb on + strand at 3291.198 kb on - strand at 3291.198 kb on - strand at 3291.203 kb on + strand at 3291.203 kb on + strand at 3291.204 kb on - strand at 3291.378 kb on + strand, within RR42_RS34950 at 3291.396 kb on + strand, within RR42_RS34950 at 3291.396 kb on + strand, within RR42_RS34950 at 3291.397 kb on - strand, within RR42_RS34950 at 3291.397 kb on - strand, within RR42_RS34950 at 3291.398 kb on + strand, within RR42_RS34950
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 3,289,023 + RR42_RS34930 0.80 -0.5 3,289,086 + RR42_RS34930 0.85 -2.3 3,289,086 + RR42_RS34930 0.85 +1.1 3,289,087 - RR42_RS34930 0.85 +0.7 3,289,087 - RR42_RS34930 0.85 +0.0 3,289,087 - RR42_RS34930 0.85 +0.6 3,289,087 - RR42_RS34930 0.85 -2.1 3,289,087 - RR42_RS34930 0.85 +0.3 3,289,087 - RR42_RS34930 0.85 -0.5 3,289,228 + -0.7 3,289,228 + -0.9 3,289,229 - +0.2 3,289,230 + +2.2 3,289,230 + -1.4 3,289,231 - +0.5 3,289,231 - -0.1 3,289,231 - +0.2 3,289,297 - -0.7 3,289,531 + RR42_RS34935 0.70 +0.4 3,289,549 + RR42_RS34935 0.76 -0.4 3,289,549 + RR42_RS34935 0.76 -0.6 3,289,549 + RR42_RS34935 0.76 +0.3 3,289,606 + +1.3 3,289,606 + -0.6 3,289,769 - -0.1 3,290,259 + RR42_RS34940 0.76 -0.4 3,290,259 + RR42_RS34940 0.76 -0.3 3,290,664 + RR42_RS34945 0.32 +0.8 3,290,665 - RR42_RS34945 0.32 -0.2 3,290,940 + RR42_RS34945 0.75 +0.2 3,291,197 + +0.4 3,291,197 + +0.3 3,291,197 + +0.1 3,291,198 - -0.8 3,291,198 - +0.8 3,291,203 + +0.2 3,291,203 + -0.7 3,291,204 - -1.0 3,291,378 + RR42_RS34950 0.16 -1.4 3,291,396 + RR42_RS34950 0.17 +0.0 3,291,396 + RR42_RS34950 0.17 +0.8 3,291,397 - RR42_RS34950 0.17 +0.3 3,291,397 - RR42_RS34950 0.17 +0.4 3,291,398 + RR42_RS34950 0.17 -0.2
Or see this region's nucleotide sequence