Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32600 and RR42_RS32605 are separated by 680 nucleotides RR42_RS32605 and RR42_RS32610 are separated by 122 nucleotides
RR42_RS32600: RR42_RS32600 - cytochrome O ubiquinol oxidase subunit II, at 2,709,779 to 2,710,747
_RS32600
RR42_RS32605: RR42_RS32605 - sulfonate ABC transporter substrate-binding protein, at 2,711,428 to 2,712,396
_RS32605
RR42_RS32610: RR42_RS32610 - O-acetylhomoserine aminocarboxypropyltransferase, at 2,712,519 to 2,713,793
_RS32610
Position (kb)
2711
2712
2713 Strain fitness (log2 ratio)
-2
-1
0
1 at 2710.739 kb on + strand at 2711.317 kb on + strand at 2711.331 kb on - strand at 2711.382 kb on - strand at 2711.382 kb on - strand at 2711.740 kb on + strand, within RR42_RS32605 at 2711.740 kb on + strand, within RR42_RS32605 at 2711.740 kb on + strand, within RR42_RS32605 at 2711.866 kb on + strand, within RR42_RS32605 at 2711.867 kb on - strand, within RR42_RS32605 at 2711.867 kb on - strand, within RR42_RS32605 at 2711.867 kb on - strand, within RR42_RS32605 at 2711.926 kb on + strand, within RR42_RS32605 at 2712.001 kb on + strand, within RR42_RS32605 at 2712.002 kb on - strand, within RR42_RS32605 at 2712.046 kb on + strand, within RR42_RS32605 at 2712.047 kb on - strand, within RR42_RS32605 at 2712.047 kb on - strand, within RR42_RS32605 at 2712.047 kb on - strand, within RR42_RS32605 at 2712.076 kb on + strand, within RR42_RS32605 at 2712.076 kb on + strand, within RR42_RS32605 at 2712.076 kb on + strand, within RR42_RS32605 at 2712.077 kb on - strand, within RR42_RS32605 at 2712.077 kb on - strand, within RR42_RS32605 at 2712.077 kb on - strand, within RR42_RS32605 at 2712.077 kb on - strand, within RR42_RS32605 at 2712.077 kb on - strand, within RR42_RS32605 at 2712.404 kb on + strand at 2712.405 kb on - strand at 2712.468 kb on + strand at 2712.468 kb on + strand at 2712.469 kb on - strand at 2712.489 kb on + strand at 2712.490 kb on - strand at 2712.490 kb on - strand at 2712.490 kb on - strand at 2712.490 kb on - strand at 2712.490 kb on - strand at 2712.493 kb on + strand at 2712.494 kb on - strand at 2712.594 kb on + strand at 2712.595 kb on - strand at 2712.668 kb on + strand, within RR42_RS32610 at 2712.669 kb on - strand, within RR42_RS32610 at 2712.672 kb on + strand, within RR42_RS32610 at 2712.673 kb on - strand, within RR42_RS32610 at 2712.673 kb on - strand, within RR42_RS32610 at 2712.673 kb on - strand, within RR42_RS32610 at 2712.673 kb on - strand, within RR42_RS32610 at 2712.673 kb on - strand, within RR42_RS32610 at 2712.673 kb on - strand, within RR42_RS32610 at 2712.780 kb on + strand, within RR42_RS32610 at 2712.780 kb on + strand, within RR42_RS32610 at 2712.780 kb on + strand, within RR42_RS32610 at 2712.837 kb on + strand, within RR42_RS32610 at 2712.849 kb on + strand, within RR42_RS32610 at 2712.849 kb on + strand, within RR42_RS32610 at 2712.850 kb on - strand, within RR42_RS32610 at 2712.850 kb on - strand, within RR42_RS32610 at 2712.850 kb on - strand, within RR42_RS32610 at 2712.850 kb on - strand, within RR42_RS32610 at 2713.029 kb on - strand, within RR42_RS32610 at 2713.029 kb on - strand, within RR42_RS32610 at 2713.074 kb on + strand, within RR42_RS32610 at 2713.074 kb on + strand, within RR42_RS32610 at 2713.075 kb on - strand, within RR42_RS32610 at 2713.131 kb on + strand, within RR42_RS32610 at 2713.132 kb on - strand, within RR42_RS32610 at 2713.242 kb on + strand, within RR42_RS32610 at 2713.284 kb on + strand, within RR42_RS32610 at 2713.285 kb on - strand, within RR42_RS32610 at 2713.285 kb on - strand, within RR42_RS32610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 2,710,739 + -1.9 2,711,317 + -0.0 2,711,331 - +0.2 2,711,382 - +0.2 2,711,382 - +0.4 2,711,740 + RR42_RS32605 0.32 +0.1 2,711,740 + RR42_RS32605 0.32 -0.5 2,711,740 + RR42_RS32605 0.32 -1.2 2,711,866 + RR42_RS32605 0.45 -0.1 2,711,867 - RR42_RS32605 0.45 -1.8 2,711,867 - RR42_RS32605 0.45 -0.3 2,711,867 - RR42_RS32605 0.45 +0.2 2,711,926 + RR42_RS32605 0.51 -0.9 2,712,001 + RR42_RS32605 0.59 -1.2 2,712,002 - RR42_RS32605 0.59 +0.1 2,712,046 + RR42_RS32605 0.64 -0.7 2,712,047 - RR42_RS32605 0.64 +0.8 2,712,047 - RR42_RS32605 0.64 +0.4 2,712,047 - RR42_RS32605 0.64 +0.3 2,712,076 + RR42_RS32605 0.67 +0.1 2,712,076 + RR42_RS32605 0.67 +0.5 2,712,076 + RR42_RS32605 0.67 -0.2 2,712,077 - RR42_RS32605 0.67 -0.8 2,712,077 - RR42_RS32605 0.67 -0.1 2,712,077 - RR42_RS32605 0.67 -0.7 2,712,077 - RR42_RS32605 0.67 -0.6 2,712,077 - RR42_RS32605 0.67 +0.8 2,712,404 + -0.0 2,712,405 - -0.8 2,712,468 + -0.7 2,712,468 + +0.0 2,712,469 - -0.4 2,712,489 + +0.0 2,712,490 - -0.6 2,712,490 - -1.2 2,712,490 - -1.5 2,712,490 - +1.2 2,712,490 - -0.1 2,712,493 + -0.9 2,712,494 - -0.1 2,712,594 + -1.2 2,712,595 - -1.5 2,712,668 + RR42_RS32610 0.12 -0.5 2,712,669 - RR42_RS32610 0.12 -0.2 2,712,672 + RR42_RS32610 0.12 -0.0 2,712,673 - RR42_RS32610 0.12 -0.2 2,712,673 - RR42_RS32610 0.12 +0.2 2,712,673 - RR42_RS32610 0.12 -0.1 2,712,673 - RR42_RS32610 0.12 -0.3 2,712,673 - RR42_RS32610 0.12 -0.8 2,712,673 - RR42_RS32610 0.12 -0.2 2,712,780 + RR42_RS32610 0.20 +0.6 2,712,780 + RR42_RS32610 0.20 -0.6 2,712,780 + RR42_RS32610 0.20 -0.0 2,712,837 + RR42_RS32610 0.25 -0.1 2,712,849 + RR42_RS32610 0.26 -1.1 2,712,849 + RR42_RS32610 0.26 -2.2 2,712,850 - RR42_RS32610 0.26 -0.9 2,712,850 - RR42_RS32610 0.26 +0.0 2,712,850 - RR42_RS32610 0.26 +0.2 2,712,850 - RR42_RS32610 0.26 -0.4 2,713,029 - RR42_RS32610 0.40 -0.3 2,713,029 - RR42_RS32610 0.40 +0.6 2,713,074 + RR42_RS32610 0.44 -0.1 2,713,074 + RR42_RS32610 0.44 +0.6 2,713,075 - RR42_RS32610 0.44 +0.7 2,713,131 + RR42_RS32610 0.48 -0.7 2,713,132 - RR42_RS32610 0.48 +0.6 2,713,242 + RR42_RS32610 0.57 -0.2 2,713,284 + RR42_RS32610 0.60 -0.0 2,713,285 - RR42_RS32610 0.60 -0.5 2,713,285 - RR42_RS32610 0.60 -0.7
Or see this region's nucleotide sequence