Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31570 and RR42_RS31575 are separated by 151 nucleotides RR42_RS31575 and RR42_RS31580 are separated by 73 nucleotides RR42_RS31580 and RR42_RS31585 are separated by 120 nucleotides
RR42_RS31570: RR42_RS31570 - Asp/Glu/hydantoin racemase, at 2,462,201 to 2,462,821
_RS31570
RR42_RS31575: RR42_RS31575 - GntR family transcriptional regulator, at 2,462,973 to 2,463,656
_RS31575
RR42_RS31580: RR42_RS31580 - 3-hydroxybutyryl-CoA dehydratase, at 2,463,730 to 2,464,494
_RS31580
RR42_RS31585: RR42_RS31585 - ABC transporter substrate-binding protein, at 2,464,615 to 2,465,568
_RS31585
Position (kb)
2463
2464
2465 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2462.799 kb on + strand at 2462.806 kb on - strand at 2462.931 kb on + strand at 2463.007 kb on + strand at 2463.008 kb on - strand at 2463.188 kb on - strand, within RR42_RS31575 at 2463.232 kb on + strand, within RR42_RS31575 at 2463.233 kb on - strand, within RR42_RS31575 at 2463.850 kb on - strand, within RR42_RS31580 at 2463.910 kb on + strand, within RR42_RS31580 at 2463.910 kb on + strand, within RR42_RS31580 at 2463.910 kb on + strand, within RR42_RS31580 at 2464.042 kb on + strand, within RR42_RS31580 at 2464.142 kb on - strand, within RR42_RS31580 at 2464.162 kb on + strand, within RR42_RS31580 at 2464.162 kb on + strand, within RR42_RS31580 at 2464.162 kb on + strand, within RR42_RS31580 at 2464.163 kb on - strand, within RR42_RS31580 at 2464.163 kb on - strand, within RR42_RS31580 at 2464.163 kb on - strand, within RR42_RS31580 at 2464.492 kb on + strand at 2464.493 kb on - strand at 2464.625 kb on + strand at 2464.625 kb on + strand at 2464.626 kb on - strand at 2464.745 kb on - strand, within RR42_RS31585 at 2464.855 kb on + strand, within RR42_RS31585 at 2464.855 kb on + strand, within RR42_RS31585 at 2464.856 kb on - strand, within RR42_RS31585 at 2464.856 kb on - strand, within RR42_RS31585 at 2464.856 kb on - strand, within RR42_RS31585 at 2464.864 kb on + strand, within RR42_RS31585 at 2464.864 kb on + strand, within RR42_RS31585 at 2464.864 kb on + strand, within RR42_RS31585 at 2464.864 kb on + strand, within RR42_RS31585 at 2464.867 kb on - strand, within RR42_RS31585 at 2464.906 kb on + strand, within RR42_RS31585 at 2464.907 kb on - strand, within RR42_RS31585 at 2464.921 kb on + strand, within RR42_RS31585 at 2465.056 kb on + strand, within RR42_RS31585 at 2465.056 kb on + strand, within RR42_RS31585 at 2465.057 kb on - strand, within RR42_RS31585 at 2465.057 kb on - strand, within RR42_RS31585 at 2465.130 kb on + strand, within RR42_RS31585 at 2465.143 kb on + strand, within RR42_RS31585 at 2465.144 kb on - strand, within RR42_RS31585 at 2465.144 kb on - strand, within RR42_RS31585 at 2465.296 kb on + strand, within RR42_RS31585 at 2465.297 kb on - strand, within RR42_RS31585 at 2465.330 kb on - strand, within RR42_RS31585 at 2465.347 kb on + strand, within RR42_RS31585 at 2465.347 kb on + strand, within RR42_RS31585
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 2,462,799 + -1.0 2,462,806 - -0.7 2,462,931 + +0.7 2,463,007 + -0.6 2,463,008 - +0.3 2,463,188 - RR42_RS31575 0.31 -0.3 2,463,232 + RR42_RS31575 0.38 +0.2 2,463,233 - RR42_RS31575 0.38 +0.3 2,463,850 - RR42_RS31580 0.16 -1.0 2,463,910 + RR42_RS31580 0.24 +0.0 2,463,910 + RR42_RS31580 0.24 +0.5 2,463,910 + RR42_RS31580 0.24 +0.2 2,464,042 + RR42_RS31580 0.41 -3.5 2,464,142 - RR42_RS31580 0.54 +0.9 2,464,162 + RR42_RS31580 0.56 +0.4 2,464,162 + RR42_RS31580 0.56 -0.3 2,464,162 + RR42_RS31580 0.56 -1.1 2,464,163 - RR42_RS31580 0.57 -1.0 2,464,163 - RR42_RS31580 0.57 -1.5 2,464,163 - RR42_RS31580 0.57 -0.9 2,464,492 + -1.1 2,464,493 - +1.3 2,464,625 + +0.6 2,464,625 + -0.2 2,464,626 - -0.4 2,464,745 - RR42_RS31585 0.14 +0.6 2,464,855 + RR42_RS31585 0.25 -0.4 2,464,855 + RR42_RS31585 0.25 -0.0 2,464,856 - RR42_RS31585 0.25 +0.0 2,464,856 - RR42_RS31585 0.25 -0.2 2,464,856 - RR42_RS31585 0.25 -0.1 2,464,864 + RR42_RS31585 0.26 -2.6 2,464,864 + RR42_RS31585 0.26 -0.9 2,464,864 + RR42_RS31585 0.26 +0.9 2,464,864 + RR42_RS31585 0.26 -0.7 2,464,867 - RR42_RS31585 0.26 -0.5 2,464,906 + RR42_RS31585 0.31 -3.1 2,464,907 - RR42_RS31585 0.31 +0.3 2,464,921 + RR42_RS31585 0.32 -1.8 2,465,056 + RR42_RS31585 0.46 +0.1 2,465,056 + RR42_RS31585 0.46 +0.4 2,465,057 - RR42_RS31585 0.46 -2.7 2,465,057 - RR42_RS31585 0.46 -1.0 2,465,130 + RR42_RS31585 0.54 -0.3 2,465,143 + RR42_RS31585 0.55 -1.1 2,465,144 - RR42_RS31585 0.55 +0.2 2,465,144 - RR42_RS31585 0.55 +0.8 2,465,296 + RR42_RS31585 0.71 -0.3 2,465,297 - RR42_RS31585 0.71 -0.2 2,465,330 - RR42_RS31585 0.75 -0.1 2,465,347 + RR42_RS31585 0.77 -1.3 2,465,347 + RR42_RS31585 0.77 +0.7
Or see this region's nucleotide sequence