Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30730 and RR42_RS30735 are separated by 501 nucleotides RR42_RS30735 and RR42_RS30740 are separated by 141 nucleotides RR42_RS30740 and RR42_RS30745 are separated by 252 nucleotides
RR42_RS30730: RR42_RS30730 - hypothetical protein, at 2,271,144 to 2,272,472
_RS30730
RR42_RS30735: RR42_RS30735 - tRNA-Met, at 2,272,974 to 2,273,049
_RS30735
RR42_RS30740: RR42_RS30740 - hypothetical protein, at 2,273,191 to 2,273,403
_RS30740
RR42_RS30745: RR42_RS30745 - hypothetical protein, at 2,273,656 to 2,275,734
_RS30745
Position (kb)
2272
2273
2274 Strain fitness (log2 ratio)
-2
-1
0
1 at 2272.138 kb on + strand, within RR42_RS30730 at 2272.138 kb on + strand, within RR42_RS30730 at 2272.139 kb on - strand, within RR42_RS30730 at 2272.139 kb on - strand, within RR42_RS30730 at 2272.182 kb on + strand, within RR42_RS30730 at 2272.300 kb on - strand, within RR42_RS30730 at 2272.324 kb on + strand, within RR42_RS30730 at 2272.482 kb on + strand at 2272.482 kb on + strand at 2272.483 kb on - strand at 2272.483 kb on - strand at 2272.483 kb on - strand at 2272.652 kb on + strand at 2272.709 kb on + strand at 2272.741 kb on + strand at 2272.741 kb on + strand at 2272.778 kb on + strand at 2272.778 kb on + strand at 2272.778 kb on + strand at 2272.779 kb on - strand at 2272.779 kb on - strand at 2272.779 kb on - strand at 2273.013 kb on + strand, within RR42_RS30735 at 2273.058 kb on + strand at 2273.060 kb on + strand at 2273.060 kb on + strand at 2273.060 kb on + strand at 2273.061 kb on - strand at 2273.061 kb on - strand at 2273.089 kb on + strand at 2273.090 kb on - strand at 2273.155 kb on + strand at 2273.155 kb on + strand at 2273.156 kb on - strand at 2273.156 kb on - strand at 2273.156 kb on - strand at 2273.293 kb on + strand, within RR42_RS30740 at 2273.294 kb on - strand, within RR42_RS30740 at 2273.368 kb on + strand, within RR42_RS30740 at 2273.368 kb on + strand, within RR42_RS30740 at 2273.368 kb on + strand, within RR42_RS30740 at 2273.369 kb on - strand, within RR42_RS30740 at 2273.369 kb on - strand, within RR42_RS30740 at 2273.369 kb on - strand, within RR42_RS30740 at 2273.369 kb on - strand, within RR42_RS30740 at 2273.369 kb on - strand, within RR42_RS30740 at 2273.372 kb on + strand, within RR42_RS30740 at 2273.372 kb on + strand, within RR42_RS30740 at 2273.373 kb on - strand, within RR42_RS30740 at 2273.373 kb on - strand, within RR42_RS30740 at 2273.373 kb on - strand, within RR42_RS30740 at 2273.373 kb on - strand, within RR42_RS30740 at 2273.381 kb on + strand, within RR42_RS30740 at 2273.382 kb on - strand at 2273.453 kb on + strand at 2273.454 kb on - strand at 2273.454 kb on - strand at 2273.454 kb on - strand at 2273.569 kb on - strand at 2273.569 kb on - strand at 2273.582 kb on + strand at 2273.582 kb on + strand at 2273.582 kb on + strand at 2273.625 kb on + strand at 2273.625 kb on + strand at 2273.626 kb on - strand at 2273.626 kb on - strand at 2273.626 kb on - strand at 2273.630 kb on - strand at 2273.635 kb on + strand at 2273.635 kb on + strand at 2273.635 kb on + strand at 2273.638 kb on + strand at 2273.639 kb on - strand at 2273.661 kb on + strand at 2273.662 kb on - strand at 2273.664 kb on + strand at 2273.665 kb on - strand at 2273.665 kb on - strand at 2273.738 kb on + strand at 2273.739 kb on - strand at 2273.798 kb on + strand at 2273.832 kb on + strand at 2273.857 kb on + strand at 2273.857 kb on + strand at 2273.858 kb on - strand at 2273.989 kb on + strand, within RR42_RS30745 at 2273.990 kb on - strand, within RR42_RS30745 at 2273.990 kb on - strand, within RR42_RS30745 at 2273.990 kb on - strand, within RR42_RS30745 at 2273.990 kb on - strand, within RR42_RS30745 at 2273.990 kb on - strand, within RR42_RS30745
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 2,272,138 + RR42_RS30730 0.75 -0.2 2,272,138 + RR42_RS30730 0.75 -0.1 2,272,139 - RR42_RS30730 0.75 +0.1 2,272,139 - RR42_RS30730 0.75 +0.4 2,272,182 + RR42_RS30730 0.78 -0.1 2,272,300 - RR42_RS30730 0.87 +0.7 2,272,324 + RR42_RS30730 0.89 +0.2 2,272,482 + -0.3 2,272,482 + -0.4 2,272,483 - +0.2 2,272,483 - +0.3 2,272,483 - +0.2 2,272,652 + -0.7 2,272,709 + +0.3 2,272,741 + -1.0 2,272,741 + -0.3 2,272,778 + -0.4 2,272,778 + -0.3 2,272,778 + +0.3 2,272,779 - -0.0 2,272,779 - -1.4 2,272,779 - +1.4 2,273,013 + RR42_RS30735 0.51 -1.2 2,273,058 + -0.0 2,273,060 + -1.0 2,273,060 + -0.2 2,273,060 + +0.6 2,273,061 - +1.1 2,273,061 - -0.2 2,273,089 + +0.7 2,273,090 - -0.7 2,273,155 + +0.3 2,273,155 + -0.1 2,273,156 - -0.9 2,273,156 - +0.6 2,273,156 - -0.5 2,273,293 + RR42_RS30740 0.48 +0.4 2,273,294 - RR42_RS30740 0.48 -0.7 2,273,368 + RR42_RS30740 0.83 -0.4 2,273,368 + RR42_RS30740 0.83 +0.1 2,273,368 + RR42_RS30740 0.83 -1.2 2,273,369 - RR42_RS30740 0.84 -0.4 2,273,369 - RR42_RS30740 0.84 -0.3 2,273,369 - RR42_RS30740 0.84 -0.4 2,273,369 - RR42_RS30740 0.84 -0.3 2,273,369 - RR42_RS30740 0.84 +0.1 2,273,372 + RR42_RS30740 0.85 +0.1 2,273,372 + RR42_RS30740 0.85 -1.2 2,273,373 - RR42_RS30740 0.85 -0.4 2,273,373 - RR42_RS30740 0.85 +1.3 2,273,373 - RR42_RS30740 0.85 +0.0 2,273,373 - RR42_RS30740 0.85 -0.4 2,273,381 + RR42_RS30740 0.89 +0.6 2,273,382 - -0.4 2,273,453 + -0.4 2,273,454 - -0.3 2,273,454 - +1.0 2,273,454 - -0.3 2,273,569 - -0.1 2,273,569 - +0.4 2,273,582 + -0.6 2,273,582 + -0.5 2,273,582 + -1.8 2,273,625 + +0.2 2,273,625 + -0.9 2,273,626 - +0.6 2,273,626 - -0.4 2,273,626 - -1.4 2,273,630 - -0.4 2,273,635 + +0.5 2,273,635 + +0.6 2,273,635 + -0.2 2,273,638 + -0.1 2,273,639 - -0.8 2,273,661 + +0.2 2,273,662 - -0.7 2,273,664 + -1.4 2,273,665 - -2.2 2,273,665 - -0.1 2,273,738 + -0.5 2,273,739 - +0.4 2,273,798 + -1.2 2,273,832 + -0.3 2,273,857 + -0.2 2,273,857 + +0.2 2,273,858 - +0.1 2,273,989 + RR42_RS30745 0.16 +0.1 2,273,990 - RR42_RS30745 0.16 -1.1 2,273,990 - RR42_RS30745 0.16 -0.3 2,273,990 - RR42_RS30745 0.16 +0.9 2,273,990 - RR42_RS30745 0.16 +1.0 2,273,990 - RR42_RS30745 0.16 +0.5
Or see this region's nucleotide sequence