Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS27850

Experiment: R2A with nitrate 150 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS27840 and RR42_RS27845 are separated by 65 nucleotidesRR42_RS27845 and RR42_RS27850 are separated by 63 nucleotidesRR42_RS27850 and RR42_RS27855 are separated by 21 nucleotidesRR42_RS27855 and RR42_RS27860 are separated by 116 nucleotides RR42_RS27840: RR42_RS27840 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 1,610,206 to 1,611,054 _RS27840 RR42_RS27845: RR42_RS27845 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 1,611,120 to 1,611,509 _RS27845 RR42_RS27850: RR42_RS27850 - 2-oxo-hepta-3-ene-1,7-dioate hydratase, at 1,611,573 to 1,612,376 _RS27850 RR42_RS27855: RR42_RS27855 - 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase, at 1,612,398 to 1,613,201 _RS27855 RR42_RS27860: RR42_RS27860 - ABC transporter substrate-binding protein, at 1,613,318 to 1,614,283 _RS27860 Position (kb) 1611 1612 1613Strain fitness (log2 ratio) -2 -1 0 1 2at 1610.603 kb on - strand, within RR42_RS27840at 1610.897 kb on - strand, within RR42_RS27840at 1610.972 kb on - strandat 1610.973 kb on - strandat 1611.142 kb on - strandat 1611.298 kb on - strand, within RR42_RS27845at 1611.298 kb on - strand, within RR42_RS27845at 1611.406 kb on + strand, within RR42_RS27845at 1611.406 kb on + strand, within RR42_RS27845at 1611.406 kb on + strand, within RR42_RS27845at 1611.407 kb on - strand, within RR42_RS27845at 1611.409 kb on - strand, within RR42_RS27845at 1611.409 kb on - strand, within RR42_RS27845at 1611.468 kb on + strand, within RR42_RS27845at 1611.690 kb on + strand, within RR42_RS27850at 1611.811 kb on - strand, within RR42_RS27850at 1611.954 kb on + strand, within RR42_RS27850at 1611.954 kb on + strand, within RR42_RS27850at 1611.955 kb on - strand, within RR42_RS27850at 1611.969 kb on + strand, within RR42_RS27850at 1611.969 kb on + strand, within RR42_RS27850at 1612.322 kb on + strandat 1612.415 kb on + strandat 1612.482 kb on + strand, within RR42_RS27855at 1612.483 kb on - strand, within RR42_RS27855at 1613.071 kb on - strand, within RR42_RS27855at 1613.197 kb on - strandat 1613.199 kb on + strandat 1613.200 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM
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1,610,603 - RR42_RS27840 0.47 -0.3
1,610,897 - RR42_RS27840 0.81 +0.0
1,610,972 - +0.2
1,610,973 - +0.7
1,611,142 - -1.6
1,611,298 - RR42_RS27845 0.46 -2.3
1,611,298 - RR42_RS27845 0.46 +0.0
1,611,406 + RR42_RS27845 0.73 +0.1
1,611,406 + RR42_RS27845 0.73 -0.2
1,611,406 + RR42_RS27845 0.73 +2.2
1,611,407 - RR42_RS27845 0.74 +0.3
1,611,409 - RR42_RS27845 0.74 +0.9
1,611,409 - RR42_RS27845 0.74 +0.1
1,611,468 + RR42_RS27845 0.89 -0.8
1,611,690 + RR42_RS27850 0.15 +0.4
1,611,811 - RR42_RS27850 0.30 +0.2
1,611,954 + RR42_RS27850 0.47 +0.3
1,611,954 + RR42_RS27850 0.47 -0.5
1,611,955 - RR42_RS27850 0.48 +0.1
1,611,969 + RR42_RS27850 0.49 +0.5
1,611,969 + RR42_RS27850 0.49 +0.3
1,612,322 + -0.1
1,612,415 + +0.7
1,612,482 + RR42_RS27855 0.10 -0.7
1,612,483 - RR42_RS27855 0.11 -0.2
1,613,071 - RR42_RS27855 0.84 -1.6
1,613,197 - +0.2
1,613,199 + -0.4
1,613,200 - +0.4

Or see this region's nucleotide sequence