Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23590 and RR42_RS23595 are separated by 187 nucleotides RR42_RS23595 and RR42_RS23600 overlap by 4 nucleotides RR42_RS23600 and RR42_RS23605 overlap by 4 nucleotides
RR42_RS23590: RR42_RS23590 - FkbM family methyltransferase, at 617,882 to 618,952
_RS23590
RR42_RS23595: RR42_RS23595 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 619,140 to 619,685
_RS23595
RR42_RS23600: RR42_RS23600 - SAM-dependent methyltransferase, at 619,682 to 620,965
_RS23600
RR42_RS23605: RR42_RS23605 - CDP-glucose 4,6-dehydratase, at 620,962 to 622,020
_RS23605
Position (kb) 619
620
621 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 618.783 kb on + strand, within RR42_RS23590 at 618.783 kb on + strand, within RR42_RS23590 at 618.783 kb on + strand, within RR42_RS23590 at 618.784 kb on - strand, within RR42_RS23590 at 618.784 kb on - strand, within RR42_RS23590 at 619.120 kb on + strand at 619.291 kb on + strand, within RR42_RS23595 at 619.292 kb on - strand, within RR42_RS23595 at 619.292 kb on - strand, within RR42_RS23595 at 619.351 kb on + strand, within RR42_RS23595 at 619.402 kb on + strand, within RR42_RS23595 at 619.415 kb on - strand, within RR42_RS23595 at 619.798 kb on - strand at 619.896 kb on + strand, within RR42_RS23600 at 619.897 kb on - strand, within RR42_RS23600 at 619.897 kb on - strand, within RR42_RS23600 at 619.927 kb on - strand, within RR42_RS23600 at 619.939 kb on + strand, within RR42_RS23600 at 619.939 kb on + strand, within RR42_RS23600 at 619.940 kb on - strand, within RR42_RS23600 at 619.963 kb on - strand, within RR42_RS23600 at 620.250 kb on + strand, within RR42_RS23600 at 620.250 kb on + strand, within RR42_RS23600 at 620.251 kb on - strand, within RR42_RS23600 at 620.256 kb on + strand, within RR42_RS23600 at 620.568 kb on + strand, within RR42_RS23600 at 620.569 kb on - strand, within RR42_RS23600 at 620.655 kb on + strand, within RR42_RS23600 at 620.688 kb on + strand, within RR42_RS23600 at 620.700 kb on + strand, within RR42_RS23600 at 620.707 kb on - strand, within RR42_RS23600 at 620.754 kb on - strand, within RR42_RS23600 at 621.036 kb on - strand at 621.314 kb on + strand, within RR42_RS23605 at 621.555 kb on - strand, within RR42_RS23605 at 621.555 kb on - strand, within RR42_RS23605 at 621.555 kb on - strand, within RR42_RS23605 at 621.618 kb on - strand, within RR42_RS23605 at 621.911 kb on + strand, within RR42_RS23605 at 621.947 kb on - strand at 621.947 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 618,783 + RR42_RS23590 0.84 +0.6 618,783 + RR42_RS23590 0.84 -1.3 618,783 + RR42_RS23590 0.84 +0.4 618,784 - RR42_RS23590 0.84 +0.3 618,784 - RR42_RS23590 0.84 +0.1 619,120 + -0.1 619,291 + RR42_RS23595 0.28 -0.1 619,292 - RR42_RS23595 0.28 -0.1 619,292 - RR42_RS23595 0.28 -0.7 619,351 + RR42_RS23595 0.39 +0.1 619,402 + RR42_RS23595 0.48 +1.3 619,415 - RR42_RS23595 0.50 -2.8 619,798 - -0.4 619,896 + RR42_RS23600 0.17 +0.1 619,897 - RR42_RS23600 0.17 +0.1 619,897 - RR42_RS23600 0.17 -0.5 619,927 - RR42_RS23600 0.19 +0.8 619,939 + RR42_RS23600 0.20 +0.5 619,939 + RR42_RS23600 0.20 +0.2 619,940 - RR42_RS23600 0.20 +0.3 619,963 - RR42_RS23600 0.22 +0.5 620,250 + RR42_RS23600 0.44 -0.5 620,250 + RR42_RS23600 0.44 +0.8 620,251 - RR42_RS23600 0.44 +0.6 620,256 + RR42_RS23600 0.45 +0.2 620,568 + RR42_RS23600 0.69 -0.2 620,569 - RR42_RS23600 0.69 +0.2 620,655 + RR42_RS23600 0.76 +0.4 620,688 + RR42_RS23600 0.78 +0.3 620,700 + RR42_RS23600 0.79 +0.5 620,707 - RR42_RS23600 0.80 +0.0 620,754 - RR42_RS23600 0.83 -0.3 621,036 - +0.7 621,314 + RR42_RS23605 0.33 +0.7 621,555 - RR42_RS23605 0.56 +0.2 621,555 - RR42_RS23605 0.56 +0.2 621,555 - RR42_RS23605 0.56 +1.8 621,618 - RR42_RS23605 0.62 -0.7 621,911 + RR42_RS23605 0.90 +0.3 621,947 - -1.3 621,947 - +0.6
Or see this region's nucleotide sequence