Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23105 and RR42_RS23110 are separated by 193 nucleotides RR42_RS23110 and RR42_RS23115 are separated by 149 nucleotides RR42_RS23115 and RR42_RS23120 are separated by 498 nucleotides RR42_RS23120 and RR42_RS23125 are separated by 32 nucleotides
RR42_RS23105: RR42_RS23105 - hypothetical protein, at 518,360 to 518,818
_RS23105
RR42_RS23110: RR42_RS23110 - isochorismatase, at 519,012 to 519,542
_RS23110
RR42_RS23115: RR42_RS23115 - serine hydrolase, at 519,692 to 521,401
_RS23115
RR42_RS23120: RR42_RS23120 - hypothetical protein, at 521,900 to 522,226
_RS23120
RR42_RS23125: RR42_RS23125 - hypothetical protein, at 522,259 to 522,546
_RS23125
Position (kb) 519
520
521
522 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 518.704 kb on - strand, within RR42_RS23105 at 518.918 kb on + strand at 518.918 kb on + strand at 518.918 kb on + strand at 518.982 kb on - strand at 519.317 kb on - strand, within RR42_RS23110 at 519.363 kb on - strand, within RR42_RS23110 at 519.513 kb on + strand at 519.513 kb on + strand at 519.514 kb on - strand at 519.514 kb on - strand at 519.693 kb on + strand at 519.741 kb on + strand at 519.741 kb on + strand at 519.741 kb on + strand at 519.742 kb on - strand at 519.879 kb on - strand, within RR42_RS23115 at 519.879 kb on - strand, within RR42_RS23115 at 519.995 kb on + strand, within RR42_RS23115 at 519.995 kb on + strand, within RR42_RS23115 at 520.001 kb on - strand, within RR42_RS23115 at 520.001 kb on - strand, within RR42_RS23115 at 520.001 kb on - strand, within RR42_RS23115 at 520.059 kb on + strand, within RR42_RS23115 at 520.128 kb on + strand, within RR42_RS23115 at 520.213 kb on - strand, within RR42_RS23115 at 520.240 kb on - strand, within RR42_RS23115 at 520.240 kb on - strand, within RR42_RS23115 at 520.240 kb on - strand, within RR42_RS23115 at 520.398 kb on + strand, within RR42_RS23115 at 520.398 kb on + strand, within RR42_RS23115 at 520.399 kb on - strand, within RR42_RS23115 at 520.536 kb on + strand, within RR42_RS23115 at 520.838 kb on + strand, within RR42_RS23115 at 520.839 kb on - strand, within RR42_RS23115 at 520.956 kb on + strand, within RR42_RS23115 at 520.962 kb on + strand, within RR42_RS23115 at 520.963 kb on - strand, within RR42_RS23115 at 521.145 kb on + strand, within RR42_RS23115 at 521.146 kb on - strand, within RR42_RS23115 at 521.292 kb on + strand at 521.292 kb on + strand at 521.437 kb on - strand at 521.505 kb on + strand at 521.505 kb on + strand at 521.793 kb on - strand at 521.916 kb on + strand at 521.917 kb on - strand at 521.917 kb on - strand at 522.040 kb on + strand, within RR42_RS23120 at 522.040 kb on + strand, within RR42_RS23120 at 522.040 kb on + strand, within RR42_RS23120 at 522.041 kb on - strand, within RR42_RS23120 at 522.130 kb on - strand, within RR42_RS23120 at 522.236 kb on + strand at 522.237 kb on - strand at 522.237 kb on - strand at 522.237 kb on - strand at 522.391 kb on - strand, within RR42_RS23125
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 518,704 - RR42_RS23105 0.75 +0.1 518,918 + -3.3 518,918 + +0.4 518,918 + +0.0 518,982 - +0.3 519,317 - RR42_RS23110 0.57 -2.2 519,363 - RR42_RS23110 0.66 -0.6 519,513 + +0.3 519,513 + -0.2 519,514 - -0.1 519,514 - +0.1 519,693 + -0.8 519,741 + +0.5 519,741 + +1.1 519,741 + -0.1 519,742 - -0.3 519,879 - RR42_RS23115 0.11 -1.3 519,879 - RR42_RS23115 0.11 -2.3 519,995 + RR42_RS23115 0.18 +0.5 519,995 + RR42_RS23115 0.18 +0.2 520,001 - RR42_RS23115 0.18 +0.6 520,001 - RR42_RS23115 0.18 +0.1 520,001 - RR42_RS23115 0.18 +1.5 520,059 + RR42_RS23115 0.21 -0.5 520,128 + RR42_RS23115 0.25 +0.1 520,213 - RR42_RS23115 0.30 -2.0 520,240 - RR42_RS23115 0.32 -0.2 520,240 - RR42_RS23115 0.32 -1.0 520,240 - RR42_RS23115 0.32 -0.6 520,398 + RR42_RS23115 0.41 -0.2 520,398 + RR42_RS23115 0.41 -0.5 520,399 - RR42_RS23115 0.41 +0.0 520,536 + RR42_RS23115 0.49 -0.7 520,838 + RR42_RS23115 0.67 -2.3 520,839 - RR42_RS23115 0.67 -0.7 520,956 + RR42_RS23115 0.74 -0.5 520,962 + RR42_RS23115 0.74 +1.2 520,963 - RR42_RS23115 0.74 -0.7 521,145 + RR42_RS23115 0.85 -0.1 521,146 - RR42_RS23115 0.85 +0.2 521,292 + -0.4 521,292 + -0.4 521,437 - -1.0 521,505 + -0.4 521,505 + -2.0 521,793 - -0.5 521,916 + -0.4 521,917 - +0.5 521,917 - -0.1 522,040 + RR42_RS23120 0.43 -1.6 522,040 + RR42_RS23120 0.43 +1.1 522,040 + RR42_RS23120 0.43 -1.2 522,041 - RR42_RS23120 0.43 -1.1 522,130 - RR42_RS23120 0.70 +0.0 522,236 + +0.1 522,237 - -1.6 522,237 - -1.5 522,237 - -1.0 522,391 - RR42_RS23125 0.46 -0.5
Or see this region's nucleotide sequence