Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23085 and RR42_RS23090 are separated by 33 nucleotides RR42_RS23090 and RR42_RS23095 are separated by 92 nucleotides RR42_RS23095 and RR42_RS23100 are separated by 89 nucleotides
RR42_RS23085: RR42_RS23085 - cytochrome C biogenesis protein CcdA, at 513,751 to 514,677
_RS23085
RR42_RS23090: RR42_RS23090 - 2,5-dioxovalerate dehydrogenase, at 514,711 to 516,312
_RS23090
RR42_RS23095: RR42_RS23095 - Zn-dependent hydrolase, at 516,405 to 517,169
_RS23095
RR42_RS23100: RR42_RS23100 - AraC family transcriptional regulator, at 517,259 to 518,224
_RS23100
Position (kb) 514
515
516
517 Strain fitness (log2 ratio) -2
-1
0
1
2 at 513.760 kb on - strand at 513.773 kb on - strand at 513.773 kb on - strand at 513.783 kb on - strand at 514.123 kb on + strand, within RR42_RS23085 at 514.123 kb on + strand, within RR42_RS23085 at 514.124 kb on - strand, within RR42_RS23085 at 514.124 kb on - strand, within RR42_RS23085 at 514.124 kb on - strand, within RR42_RS23085 at 514.158 kb on + strand, within RR42_RS23085 at 514.253 kb on - strand, within RR42_RS23085 at 514.284 kb on - strand, within RR42_RS23085 at 514.478 kb on - strand, within RR42_RS23085 at 514.478 kb on - strand, within RR42_RS23085 at 514.504 kb on + strand, within RR42_RS23085 at 514.504 kb on + strand, within RR42_RS23085 at 514.504 kb on + strand, within RR42_RS23085 at 514.505 kb on - strand, within RR42_RS23085 at 514.505 kb on - strand, within RR42_RS23085 at 515.018 kb on + strand, within RR42_RS23090 at 515.284 kb on - strand, within RR42_RS23090 at 515.497 kb on + strand, within RR42_RS23090 at 515.815 kb on + strand, within RR42_RS23090 at 515.816 kb on - strand, within RR42_RS23090 at 516.116 kb on - strand, within RR42_RS23090 at 516.142 kb on - strand, within RR42_RS23090 at 516.142 kb on - strand, within RR42_RS23090 at 516.190 kb on + strand at 516.191 kb on - strand at 516.304 kb on - strand at 516.428 kb on + strand at 516.428 kb on + strand at 516.675 kb on + strand, within RR42_RS23095 at 516.695 kb on - strand, within RR42_RS23095 at 516.695 kb on - strand, within RR42_RS23095 at 516.715 kb on - strand, within RR42_RS23095 at 516.715 kb on - strand, within RR42_RS23095 at 516.872 kb on - strand, within RR42_RS23095 at 517.163 kb on + strand at 517.163 kb on + strand at 517.163 kb on + strand at 517.163 kb on + strand at 517.164 kb on - strand at 517.164 kb on - strand at 517.164 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 513,760 - -0.2 513,773 - -0.2 513,773 - +1.0 513,783 - +0.2 514,123 + RR42_RS23085 0.40 -0.0 514,123 + RR42_RS23085 0.40 -2.3 514,124 - RR42_RS23085 0.40 -0.8 514,124 - RR42_RS23085 0.40 -0.7 514,124 - RR42_RS23085 0.40 +0.2 514,158 + RR42_RS23085 0.44 -1.0 514,253 - RR42_RS23085 0.54 -0.3 514,284 - RR42_RS23085 0.57 -0.4 514,478 - RR42_RS23085 0.78 -0.8 514,478 - RR42_RS23085 0.78 +0.1 514,504 + RR42_RS23085 0.81 -0.8 514,504 + RR42_RS23085 0.81 +0.3 514,504 + RR42_RS23085 0.81 +0.0 514,505 - RR42_RS23085 0.81 -1.2 514,505 - RR42_RS23085 0.81 +1.8 515,018 + RR42_RS23090 0.19 -0.1 515,284 - RR42_RS23090 0.36 +0.6 515,497 + RR42_RS23090 0.49 +0.9 515,815 + RR42_RS23090 0.69 +0.6 515,816 - RR42_RS23090 0.69 +0.2 516,116 - RR42_RS23090 0.88 +0.4 516,142 - RR42_RS23090 0.89 +0.3 516,142 - RR42_RS23090 0.89 -0.1 516,190 + -0.9 516,191 - +0.2 516,304 - +1.3 516,428 + -0.9 516,428 + +0.1 516,675 + RR42_RS23095 0.35 -0.3 516,695 - RR42_RS23095 0.38 +0.5 516,695 - RR42_RS23095 0.38 -0.3 516,715 - RR42_RS23095 0.41 -0.9 516,715 - RR42_RS23095 0.41 -1.2 516,872 - RR42_RS23095 0.61 -0.0 517,163 + -1.8 517,163 + +0.9 517,163 + -0.8 517,163 + +0.0 517,164 - +0.9 517,164 - -0.6 517,164 - -0.6
Or see this region's nucleotide sequence