Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16530 and RR42_RS16535 are separated by 36 nucleotides RR42_RS16535 and RR42_RS16540 are separated by 264 nucleotides RR42_RS16540 and RR42_RS16545 are separated by 27 nucleotides RR42_RS16545 and RR42_RS16550 are separated by 283 nucleotides
RR42_RS16530: RR42_RS16530 - metallophosphoesterase, at 3,580,760 to 3,581,704
_RS16530
RR42_RS16535: RR42_RS16535 - hypothetical protein, at 3,581,741 to 3,582,088
_RS16535
RR42_RS16540: RR42_RS16540 - penicillin-binding protein, at 3,582,353 to 3,584,542
_RS16540
RR42_RS16545: RR42_RS16545 - prolyl-tRNA synthetase, at 3,584,570 to 3,585,034
_RS16545
RR42_RS16550: RR42_RS16550 - hydroperoxidase, at 3,585,318 to 3,587,519
_RS16550
Position (kb)
3582
3583
3584
3585 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3581.405 kb on + strand, within RR42_RS16530 at 3581.406 kb on - strand, within RR42_RS16530 at 3581.453 kb on + strand, within RR42_RS16530 at 3581.454 kb on - strand, within RR42_RS16530 at 3581.527 kb on + strand, within RR42_RS16530 at 3581.527 kb on + strand, within RR42_RS16530 at 3581.527 kb on + strand, within RR42_RS16530 at 3581.528 kb on - strand, within RR42_RS16530 at 3581.540 kb on - strand, within RR42_RS16530 at 3581.745 kb on + strand at 3582.003 kb on - strand, within RR42_RS16535 at 3582.033 kb on - strand, within RR42_RS16535 at 3582.051 kb on + strand, within RR42_RS16535 at 3582.061 kb on + strand at 3582.232 kb on + strand at 3582.232 kb on + strand at 3582.233 kb on - strand at 3582.239 kb on - strand at 3582.242 kb on + strand at 3582.242 kb on + strand at 3582.242 kb on + strand at 3582.243 kb on - strand at 3582.298 kb on - strand at 3582.298 kb on - strand at 3582.429 kb on + strand at 3582.429 kb on + strand at 3582.521 kb on + strand at 3582.521 kb on + strand at 3582.521 kb on + strand at 3582.522 kb on - strand at 3582.522 kb on - strand at 3582.625 kb on + strand, within RR42_RS16540 at 3582.748 kb on + strand, within RR42_RS16540 at 3582.769 kb on + strand, within RR42_RS16540 at 3582.769 kb on + strand, within RR42_RS16540 at 3582.769 kb on + strand, within RR42_RS16540 at 3582.769 kb on + strand, within RR42_RS16540 at 3582.911 kb on + strand, within RR42_RS16540 at 3582.962 kb on + strand, within RR42_RS16540 at 3582.998 kb on + strand, within RR42_RS16540 at 3582.999 kb on - strand, within RR42_RS16540 at 3582.999 kb on - strand, within RR42_RS16540 at 3583.076 kb on + strand, within RR42_RS16540 at 3583.077 kb on - strand, within RR42_RS16540 at 3583.146 kb on - strand, within RR42_RS16540 at 3583.146 kb on - strand, within RR42_RS16540 at 3583.169 kb on + strand, within RR42_RS16540 at 3583.170 kb on - strand, within RR42_RS16540 at 3583.304 kb on + strand, within RR42_RS16540 at 3583.305 kb on - strand, within RR42_RS16540 at 3583.627 kb on + strand, within RR42_RS16540 at 3583.773 kb on + strand, within RR42_RS16540 at 3584.066 kb on + strand, within RR42_RS16540 at 3584.291 kb on + strand, within RR42_RS16540 at 3584.293 kb on + strand, within RR42_RS16540 at 3584.371 kb on + strand at 3584.375 kb on + strand at 3584.424 kb on - strand at 3584.676 kb on + strand, within RR42_RS16545 at 3584.676 kb on + strand, within RR42_RS16545 at 3584.676 kb on + strand, within RR42_RS16545 at 3584.677 kb on - strand, within RR42_RS16545 at 3584.728 kb on - strand, within RR42_RS16545 at 3584.934 kb on + strand, within RR42_RS16545 at 3584.934 kb on + strand, within RR42_RS16545 at 3584.935 kb on - strand, within RR42_RS16545 at 3584.935 kb on - strand, within RR42_RS16545 at 3585.010 kb on + strand at 3585.071 kb on + strand at 3585.071 kb on + strand at 3585.072 kb on - strand at 3585.084 kb on + strand at 3585.085 kb on - strand at 3585.085 kb on - strand at 3585.086 kb on + strand at 3585.086 kb on + strand at 3585.087 kb on - strand at 3585.087 kb on - strand at 3585.119 kb on - strand at 3585.119 kb on - strand at 3585.157 kb on + strand at 3585.266 kb on + strand at 3585.267 kb on - strand at 3585.267 kb on - strand at 3585.269 kb on + strand at 3585.270 kb on - strand at 3585.282 kb on - strand at 3585.297 kb on + strand at 3585.400 kb on + strand at 3585.452 kb on + strand at 3585.452 kb on + strand at 3585.456 kb on + strand at 3585.456 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 3,581,405 + RR42_RS16530 0.68 -2.2 3,581,406 - RR42_RS16530 0.68 -0.0 3,581,453 + RR42_RS16530 0.73 -0.2 3,581,454 - RR42_RS16530 0.73 +0.3 3,581,527 + RR42_RS16530 0.81 +0.5 3,581,527 + RR42_RS16530 0.81 -0.4 3,581,527 + RR42_RS16530 0.81 -0.0 3,581,528 - RR42_RS16530 0.81 -0.2 3,581,540 - RR42_RS16530 0.83 -1.5 3,581,745 + +0.4 3,582,003 - RR42_RS16535 0.75 -0.2 3,582,033 - RR42_RS16535 0.84 -0.0 3,582,051 + RR42_RS16535 0.89 -0.2 3,582,061 + +1.4 3,582,232 + -0.3 3,582,232 + -0.3 3,582,233 - -1.2 3,582,239 - +0.6 3,582,242 + +0.7 3,582,242 + -0.1 3,582,242 + +1.1 3,582,243 - -0.2 3,582,298 - +0.2 3,582,298 - -0.1 3,582,429 + -0.5 3,582,429 + -0.9 3,582,521 + -2.9 3,582,521 + -0.2 3,582,521 + -1.4 3,582,522 - +0.2 3,582,522 - +0.1 3,582,625 + RR42_RS16540 0.12 -1.2 3,582,748 + RR42_RS16540 0.18 -3.3 3,582,769 + RR42_RS16540 0.19 -1.3 3,582,769 + RR42_RS16540 0.19 -0.3 3,582,769 + RR42_RS16540 0.19 -0.7 3,582,769 + RR42_RS16540 0.19 -0.7 3,582,911 + RR42_RS16540 0.25 -3.6 3,582,962 + RR42_RS16540 0.28 -3.3 3,582,998 + RR42_RS16540 0.29 -0.2 3,582,999 - RR42_RS16540 0.29 -0.9 3,582,999 - RR42_RS16540 0.29 -0.4 3,583,076 + RR42_RS16540 0.33 -0.6 3,583,077 - RR42_RS16540 0.33 -0.9 3,583,146 - RR42_RS16540 0.36 +0.1 3,583,146 - RR42_RS16540 0.36 -0.8 3,583,169 + RR42_RS16540 0.37 -1.3 3,583,170 - RR42_RS16540 0.37 -1.0 3,583,304 + RR42_RS16540 0.43 -0.3 3,583,305 - RR42_RS16540 0.43 -0.2 3,583,627 + RR42_RS16540 0.58 -1.4 3,583,773 + RR42_RS16540 0.65 -3.2 3,584,066 + RR42_RS16540 0.78 -0.3 3,584,291 + RR42_RS16540 0.88 -0.5 3,584,293 + RR42_RS16540 0.89 -0.1 3,584,371 + +0.7 3,584,375 + +1.4 3,584,424 - -0.8 3,584,676 + RR42_RS16545 0.23 -0.2 3,584,676 + RR42_RS16545 0.23 -0.5 3,584,676 + RR42_RS16545 0.23 -0.1 3,584,677 - RR42_RS16545 0.23 -0.4 3,584,728 - RR42_RS16545 0.34 +0.6 3,584,934 + RR42_RS16545 0.78 +0.7 3,584,934 + RR42_RS16545 0.78 -0.0 3,584,935 - RR42_RS16545 0.78 +0.4 3,584,935 - RR42_RS16545 0.78 -0.3 3,585,010 + -0.1 3,585,071 + +0.3 3,585,071 + -0.7 3,585,072 - -0.1 3,585,084 + +0.8 3,585,085 - -0.4 3,585,085 - -0.6 3,585,086 + -1.4 3,585,086 + -0.5 3,585,087 - +0.4 3,585,087 - +1.1 3,585,119 - -0.7 3,585,119 - -0.5 3,585,157 + -1.1 3,585,266 + +1.3 3,585,267 - -0.5 3,585,267 - +1.2 3,585,269 + -0.1 3,585,270 - +0.6 3,585,282 - +0.1 3,585,297 + -0.3 3,585,400 + -0.6 3,585,452 + +0.4 3,585,452 + -1.3 3,585,456 + +0.8 3,585,456 + +0.3
Or see this region's nucleotide sequence