Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15940 and RR42_RS15945 are separated by 142 nucleotides RR42_RS15945 and RR42_RS15950 are separated by 192 nucleotides RR42_RS15950 and RR42_RS15955 are separated by 69 nucleotides RR42_RS15955 and RR42_RS15960 are separated by 13 nucleotides
RR42_RS15940: RR42_RS15940 - lysine decarboxylase, at 3,448,293 to 3,450,557
_RS15940
RR42_RS15945: RR42_RS15945 - deoxycytidine triphosphate deaminase, at 3,450,700 to 3,451,266
_RS15945
RR42_RS15950: RR42_RS15950 - formate dehydrogenase, at 3,451,459 to 3,452,289
_RS15950
RR42_RS15955: RR42_RS15955 - superoxide dismutase, at 3,452,359 to 3,452,898
_RS15955
RR42_RS15960: RR42_RS15960 - hypothetical protein, at 3,452,912 to 3,453,313
_RS15960
Position (kb)
3451
3452
3453 Strain fitness (log2 ratio)
-2
-1
0
1 at 3450.694 kb on - strand at 3450.694 kb on - strand at 3451.388 kb on + strand at 3451.389 kb on - strand at 3451.389 kb on - strand at 3451.389 kb on - strand at 3451.389 kb on - strand at 3451.389 kb on - strand at 3451.389 kb on - strand at 3451.416 kb on - strand at 3451.511 kb on + strand at 3451.512 kb on - strand at 3451.512 kb on - strand at 3451.512 kb on - strand at 3451.673 kb on + strand, within RR42_RS15950 at 3451.673 kb on + strand, within RR42_RS15950 at 3451.673 kb on + strand, within RR42_RS15950 at 3451.673 kb on + strand, within RR42_RS15950 at 3451.673 kb on + strand, within RR42_RS15950 at 3451.674 kb on + strand, within RR42_RS15950 at 3451.674 kb on - strand, within RR42_RS15950 at 3451.674 kb on - strand, within RR42_RS15950 at 3451.823 kb on + strand, within RR42_RS15950 at 3451.862 kb on + strand, within RR42_RS15950 at 3451.863 kb on - strand, within RR42_RS15950 at 3451.864 kb on + strand, within RR42_RS15950 at 3451.864 kb on + strand, within RR42_RS15950 at 3451.865 kb on - strand, within RR42_RS15950 at 3451.865 kb on - strand, within RR42_RS15950 at 3451.865 kb on - strand, within RR42_RS15950 at 3452.099 kb on + strand, within RR42_RS15950 at 3452.139 kb on + strand, within RR42_RS15950 at 3452.139 kb on + strand, within RR42_RS15950 at 3452.140 kb on - strand, within RR42_RS15950 at 3452.140 kb on - strand, within RR42_RS15950 at 3452.140 kb on - strand, within RR42_RS15950 at 3452.169 kb on - strand, within RR42_RS15950 at 3452.423 kb on + strand, within RR42_RS15955 at 3452.424 kb on - strand, within RR42_RS15955 at 3452.704 kb on + strand, within RR42_RS15955 at 3452.864 kb on - strand at 3452.882 kb on - strand at 3452.887 kb on + strand at 3452.887 kb on + strand at 3452.887 kb on + strand at 3452.888 kb on - strand at 3452.937 kb on - strand at 3453.129 kb on + strand, within RR42_RS15960 at 3453.129 kb on + strand, within RR42_RS15960 at 3453.129 kb on + strand, within RR42_RS15960 at 3453.132 kb on + strand, within RR42_RS15960
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 3,450,694 - -0.1 3,450,694 - +0.8 3,451,388 + +0.7 3,451,389 - -0.1 3,451,389 - -0.3 3,451,389 - +0.4 3,451,389 - +0.1 3,451,389 - +1.0 3,451,389 - +0.6 3,451,416 - +1.7 3,451,511 + -0.4 3,451,512 - -0.5 3,451,512 - -0.9 3,451,512 - -2.5 3,451,673 + RR42_RS15950 0.26 +0.4 3,451,673 + RR42_RS15950 0.26 +0.2 3,451,673 + RR42_RS15950 0.26 +0.3 3,451,673 + RR42_RS15950 0.26 -0.2 3,451,673 + RR42_RS15950 0.26 +0.8 3,451,674 + RR42_RS15950 0.26 +0.9 3,451,674 - RR42_RS15950 0.26 +0.1 3,451,674 - RR42_RS15950 0.26 +0.5 3,451,823 + RR42_RS15950 0.44 -0.3 3,451,862 + RR42_RS15950 0.48 +0.3 3,451,863 - RR42_RS15950 0.49 +1.6 3,451,864 + RR42_RS15950 0.49 +0.1 3,451,864 + RR42_RS15950 0.49 -0.2 3,451,865 - RR42_RS15950 0.49 -0.0 3,451,865 - RR42_RS15950 0.49 +0.8 3,451,865 - RR42_RS15950 0.49 -0.4 3,452,099 + RR42_RS15950 0.77 -0.4 3,452,139 + RR42_RS15950 0.82 +1.7 3,452,139 + RR42_RS15950 0.82 -0.2 3,452,140 - RR42_RS15950 0.82 -0.4 3,452,140 - RR42_RS15950 0.82 +0.9 3,452,140 - RR42_RS15950 0.82 +0.3 3,452,169 - RR42_RS15950 0.85 +0.8 3,452,423 + RR42_RS15955 0.12 +0.1 3,452,424 - RR42_RS15955 0.12 +0.3 3,452,704 + RR42_RS15955 0.64 -1.3 3,452,864 - +0.2 3,452,882 - +0.0 3,452,887 + -0.9 3,452,887 + +0.5 3,452,887 + -0.8 3,452,888 - +0.1 3,452,937 - +0.0 3,453,129 + RR42_RS15960 0.54 +0.0 3,453,129 + RR42_RS15960 0.54 -1.9 3,453,129 + RR42_RS15960 0.54 +1.1 3,453,132 + RR42_RS15960 0.55 -0.7
Or see this region's nucleotide sequence