Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15745 and RR42_RS15750 overlap by 4 nucleotides RR42_RS15750 and RR42_RS15755 overlap by 4 nucleotides RR42_RS15755 and RR42_RS15760 are separated by 73 nucleotides
RR42_RS15745: RR42_RS15745 - group1 glycosyl transferase, at 3,403,029 to 3,404,264
_RS15745
RR42_RS15750: RR42_RS15750 - hypothetical protein, at 3,404,261 to 3,405,469
_RS15750
RR42_RS15755: RR42_RS15755 - acetyltransferase, at 3,405,466 to 3,406,020
_RS15755
RR42_RS15760: RR42_RS15760 - hypothetical protein, at 3,406,094 to 3,407,332
_RS15760
Position (kb)
3404
3405
3406 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3403.282 kb on + strand, within RR42_RS15745 at 3403.283 kb on - strand, within RR42_RS15745 at 3403.321 kb on + strand, within RR42_RS15745 at 3403.345 kb on + strand, within RR42_RS15745 at 3403.351 kb on + strand, within RR42_RS15745 at 3403.351 kb on + strand, within RR42_RS15745 at 3403.351 kb on + strand, within RR42_RS15745 at 3403.351 kb on + strand, within RR42_RS15745 at 3403.552 kb on + strand, within RR42_RS15745 at 3403.714 kb on + strand, within RR42_RS15745 at 3403.906 kb on + strand, within RR42_RS15745 at 3403.906 kb on + strand, within RR42_RS15745 at 3403.915 kb on + strand, within RR42_RS15745 at 3404.099 kb on + strand, within RR42_RS15745 at 3404.099 kb on + strand, within RR42_RS15745 at 3404.099 kb on + strand, within RR42_RS15745 at 3404.101 kb on + strand, within RR42_RS15745 at 3404.239 kb on + strand at 3404.279 kb on - strand at 3404.340 kb on + strand at 3404.340 kb on + strand at 3404.344 kb on + strand at 3404.344 kb on + strand at 3404.344 kb on + strand at 3404.362 kb on - strand at 3404.369 kb on + strand at 3404.399 kb on + strand, within RR42_RS15750 at 3404.460 kb on + strand, within RR42_RS15750 at 3404.520 kb on + strand, within RR42_RS15750 at 3404.529 kb on + strand, within RR42_RS15750 at 3404.529 kb on + strand, within RR42_RS15750 at 3404.566 kb on + strand, within RR42_RS15750 at 3404.566 kb on + strand, within RR42_RS15750 at 3404.816 kb on + strand, within RR42_RS15750 at 3404.946 kb on + strand, within RR42_RS15750 at 3405.076 kb on + strand, within RR42_RS15750 at 3405.356 kb on + strand at 3405.356 kb on + strand at 3405.356 kb on + strand at 3405.384 kb on + strand at 3405.384 kb on + strand at 3405.387 kb on + strand at 3405.387 kb on + strand at 3405.393 kb on + strand at 3405.393 kb on + strand at 3405.393 kb on + strand at 3405.396 kb on + strand at 3405.687 kb on - strand, within RR42_RS15755 at 3405.687 kb on - strand, within RR42_RS15755 at 3405.735 kb on + strand, within RR42_RS15755 at 3405.794 kb on - strand, within RR42_RS15755 at 3406.078 kb on + strand at 3406.078 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 3,403,282 + RR42_RS15745 0.20 -3.6 3,403,283 - RR42_RS15745 0.21 -2.2 3,403,321 + RR42_RS15745 0.24 -2.1 3,403,345 + RR42_RS15745 0.26 -2.4 3,403,351 + RR42_RS15745 0.26 -2.1 3,403,351 + RR42_RS15745 0.26 -1.7 3,403,351 + RR42_RS15745 0.26 -2.0 3,403,351 + RR42_RS15745 0.26 -4.0 3,403,552 + RR42_RS15745 0.42 -2.1 3,403,714 + RR42_RS15745 0.55 -3.7 3,403,906 + RR42_RS15745 0.71 -2.8 3,403,906 + RR42_RS15745 0.71 -2.3 3,403,915 + RR42_RS15745 0.72 -2.0 3,404,099 + RR42_RS15745 0.87 -2.2 3,404,099 + RR42_RS15745 0.87 -3.6 3,404,099 + RR42_RS15745 0.87 -2.8 3,404,101 + RR42_RS15745 0.87 -0.9 3,404,239 + -1.9 3,404,279 - +0.4 3,404,340 + -0.7 3,404,340 + -2.5 3,404,344 + -1.4 3,404,344 + -1.6 3,404,344 + -1.0 3,404,362 - -2.1 3,404,369 + -1.0 3,404,399 + RR42_RS15750 0.11 -2.1 3,404,460 + RR42_RS15750 0.16 -1.4 3,404,520 + RR42_RS15750 0.21 -1.3 3,404,529 + RR42_RS15750 0.22 -2.5 3,404,529 + RR42_RS15750 0.22 -0.9 3,404,566 + RR42_RS15750 0.25 -2.0 3,404,566 + RR42_RS15750 0.25 -0.4 3,404,816 + RR42_RS15750 0.46 -1.7 3,404,946 + RR42_RS15750 0.57 -0.7 3,405,076 + RR42_RS15750 0.67 -2.4 3,405,356 + -2.9 3,405,356 + -2.1 3,405,356 + -2.5 3,405,384 + -2.3 3,405,384 + -1.2 3,405,387 + -1.4 3,405,387 + -4.5 3,405,393 + -3.2 3,405,393 + -3.2 3,405,393 + -2.3 3,405,396 + -1.0 3,405,687 - RR42_RS15755 0.40 -1.8 3,405,687 - RR42_RS15755 0.40 +0.5 3,405,735 + RR42_RS15755 0.48 +0.6 3,405,794 - RR42_RS15755 0.59 -2.0 3,406,078 + -1.1 3,406,078 + -1.8
Or see this region's nucleotide sequence