Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15725 and RR42_RS15730 are separated by 48 nucleotides RR42_RS15730 and RR42_RS15735 overlap by 1 nucleotides RR42_RS15735 and RR42_RS15740 are separated by 214 nucleotides RR42_RS15740 and RR42_RS15745 are separated by 60 nucleotides
RR42_RS15725: RR42_RS15725 - hypothetical protein, at 3,399,837 to 3,400,478
_RS15725
RR42_RS15730: RR42_RS15730 - sugar transferase, at 3,400,527 to 3,401,123
_RS15730
RR42_RS15735: RR42_RS15735 - pyridoxal phosphate-dependent aminotransferase, at 3,401,123 to 3,402,277
_RS15735
RR42_RS15740: RR42_RS15740 - hypothetical protein, at 3,402,492 to 3,402,968
_RS15740
RR42_RS15745: RR42_RS15745 - group1 glycosyl transferase, at 3,403,029 to 3,404,264
_RS15745
Position (kb)
3401
3402
3403 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3400.225 kb on + strand, within RR42_RS15725 at 3400.226 kb on - strand, within RR42_RS15725 at 3400.559 kb on + strand at 3400.598 kb on - strand, within RR42_RS15730 at 3400.598 kb on - strand, within RR42_RS15730 at 3400.598 kb on - strand, within RR42_RS15730 at 3400.610 kb on - strand, within RR42_RS15730 at 3400.641 kb on - strand, within RR42_RS15730 at 3400.642 kb on + strand, within RR42_RS15730 at 3400.643 kb on - strand, within RR42_RS15730 at 3400.643 kb on - strand, within RR42_RS15730 at 3400.762 kb on + strand, within RR42_RS15730 at 3400.774 kb on + strand, within RR42_RS15730 at 3400.774 kb on + strand, within RR42_RS15730 at 3400.775 kb on - strand, within RR42_RS15730 at 3400.775 kb on - strand, within RR42_RS15730 at 3400.775 kb on - strand, within RR42_RS15730 at 3400.775 kb on - strand, within RR42_RS15730 at 3400.858 kb on + strand, within RR42_RS15730 at 3400.858 kb on + strand, within RR42_RS15730 at 3400.890 kb on - strand, within RR42_RS15730 at 3400.958 kb on + strand, within RR42_RS15730 at 3400.958 kb on + strand, within RR42_RS15730 at 3400.959 kb on - strand, within RR42_RS15730 at 3401.059 kb on - strand, within RR42_RS15730 at 3401.129 kb on - strand at 3401.179 kb on + strand at 3401.187 kb on + strand at 3401.200 kb on + strand at 3401.200 kb on + strand at 3401.214 kb on + strand at 3401.271 kb on + strand, within RR42_RS15735 at 3401.296 kb on - strand, within RR42_RS15735 at 3401.296 kb on - strand, within RR42_RS15735 at 3401.306 kb on - strand, within RR42_RS15735 at 3401.385 kb on + strand, within RR42_RS15735 at 3401.385 kb on + strand, within RR42_RS15735 at 3401.405 kb on - strand, within RR42_RS15735 at 3401.556 kb on + strand, within RR42_RS15735 at 3401.557 kb on - strand, within RR42_RS15735 at 3401.557 kb on - strand, within RR42_RS15735 at 3401.584 kb on - strand, within RR42_RS15735 at 3401.586 kb on + strand, within RR42_RS15735 at 3401.587 kb on - strand, within RR42_RS15735 at 3401.587 kb on - strand, within RR42_RS15735 at 3401.785 kb on + strand, within RR42_RS15735 at 3401.786 kb on - strand, within RR42_RS15735 at 3401.979 kb on - strand, within RR42_RS15735 at 3402.040 kb on + strand, within RR42_RS15735 at 3402.040 kb on + strand, within RR42_RS15735 at 3402.041 kb on - strand, within RR42_RS15735 at 3402.126 kb on - strand, within RR42_RS15735 at 3402.126 kb on - strand, within RR42_RS15735 at 3402.269 kb on - strand at 3402.269 kb on - strand at 3402.280 kb on + strand at 3402.281 kb on - strand at 3402.281 kb on - strand at 3402.299 kb on - strand at 3402.312 kb on - strand at 3402.478 kb on + strand at 3402.586 kb on - strand, within RR42_RS15740 at 3402.630 kb on - strand, within RR42_RS15740 at 3402.714 kb on + strand, within RR42_RS15740 at 3402.929 kb on - strand at 3403.004 kb on - strand at 3403.004 kb on - strand at 3403.011 kb on - strand at 3403.019 kb on + strand at 3403.019 kb on + strand at 3403.019 kb on + strand at 3403.019 kb on + strand at 3403.020 kb on - strand at 3403.020 kb on - strand at 3403.020 kb on - strand at 3403.020 kb on - strand at 3403.121 kb on + strand at 3403.192 kb on + strand, within RR42_RS15745
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 3,400,225 + RR42_RS15725 0.60 -0.2 3,400,226 - RR42_RS15725 0.61 -0.4 3,400,559 + -1.8 3,400,598 - RR42_RS15730 0.12 +1.4 3,400,598 - RR42_RS15730 0.12 -2.6 3,400,598 - RR42_RS15730 0.12 -1.3 3,400,610 - RR42_RS15730 0.14 +0.1 3,400,641 - RR42_RS15730 0.19 -2.7 3,400,642 + RR42_RS15730 0.19 +0.2 3,400,643 - RR42_RS15730 0.19 -2.6 3,400,643 - RR42_RS15730 0.19 -4.2 3,400,762 + RR42_RS15730 0.39 -2.4 3,400,774 + RR42_RS15730 0.41 -0.8 3,400,774 + RR42_RS15730 0.41 -0.1 3,400,775 - RR42_RS15730 0.42 -1.6 3,400,775 - RR42_RS15730 0.42 -0.7 3,400,775 - RR42_RS15730 0.42 -2.6 3,400,775 - RR42_RS15730 0.42 -1.0 3,400,858 + RR42_RS15730 0.55 -2.2 3,400,858 + RR42_RS15730 0.55 -2.6 3,400,890 - RR42_RS15730 0.61 +0.4 3,400,958 + RR42_RS15730 0.72 -1.3 3,400,958 + RR42_RS15730 0.72 -0.7 3,400,959 - RR42_RS15730 0.72 -0.3 3,401,059 - RR42_RS15730 0.89 +0.6 3,401,129 - +1.0 3,401,179 + -0.2 3,401,187 + +0.4 3,401,200 + -1.4 3,401,200 + -2.7 3,401,214 + -1.8 3,401,271 + RR42_RS15735 0.13 -1.9 3,401,296 - RR42_RS15735 0.15 +0.9 3,401,296 - RR42_RS15735 0.15 -1.0 3,401,306 - RR42_RS15735 0.16 -1.9 3,401,385 + RR42_RS15735 0.23 -0.3 3,401,385 + RR42_RS15735 0.23 -1.2 3,401,405 - RR42_RS15735 0.24 +0.7 3,401,556 + RR42_RS15735 0.37 -0.7 3,401,557 - RR42_RS15735 0.38 -1.3 3,401,557 - RR42_RS15735 0.38 -0.9 3,401,584 - RR42_RS15735 0.40 +0.4 3,401,586 + RR42_RS15735 0.40 -1.8 3,401,587 - RR42_RS15735 0.40 -1.2 3,401,587 - RR42_RS15735 0.40 -1.2 3,401,785 + RR42_RS15735 0.57 -1.0 3,401,786 - RR42_RS15735 0.57 -0.5 3,401,979 - RR42_RS15735 0.74 -0.9 3,402,040 + RR42_RS15735 0.79 -4.3 3,402,040 + RR42_RS15735 0.79 -1.4 3,402,041 - RR42_RS15735 0.79 -1.5 3,402,126 - RR42_RS15735 0.87 -1.5 3,402,126 - RR42_RS15735 0.87 -0.4 3,402,269 - -1.7 3,402,269 - -2.3 3,402,280 + -0.2 3,402,281 - +0.5 3,402,281 - -0.1 3,402,299 - +0.4 3,402,312 - +0.5 3,402,478 + -0.8 3,402,586 - RR42_RS15740 0.20 -1.7 3,402,630 - RR42_RS15740 0.29 -1.4 3,402,714 + RR42_RS15740 0.47 -3.5 3,402,929 - -3.0 3,403,004 - -2.2 3,403,004 - -3.6 3,403,011 - -3.5 3,403,019 + -0.9 3,403,019 + -4.0 3,403,019 + -0.5 3,403,019 + -2.0 3,403,020 - -0.1 3,403,020 - +0.9 3,403,020 - +0.4 3,403,020 - +0.2 3,403,121 + -1.2 3,403,192 + RR42_RS15745 0.13 -1.6
Or see this region's nucleotide sequence