Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11275 and RR42_RS11280 are separated by 382 nucleotides RR42_RS11280 and RR42_RS11285 are separated by 112 nucleotides RR42_RS11285 and RR42_RS11290 are separated by 120 nucleotides
RR42_RS11275: RR42_RS11275 - 3-methylitaconate isomerase, at 2,412,089 to 2,413,279
_RS11275
RR42_RS11280: RR42_RS11280 - phenylacetic acid degradation protein, at 2,413,662 to 2,414,090
_RS11280
RR42_RS11285: RR42_RS11285 - thioesterase, at 2,414,203 to 2,414,640
_RS11285
RR42_RS11290: RR42_RS11290 - TetR family transcriptional regulator, at 2,414,761 to 2,415,363
_RS11290
Position (kb)
2413
2414
2415 Strain fitness (log2 ratio)
-1
0
1
2 at 2412.886 kb on + strand, within RR42_RS11275 at 2412.887 kb on - strand, within RR42_RS11275 at 2412.887 kb on - strand, within RR42_RS11275 at 2412.950 kb on + strand, within RR42_RS11275 at 2412.951 kb on - strand, within RR42_RS11275 at 2412.951 kb on - strand, within RR42_RS11275 at 2412.951 kb on - strand, within RR42_RS11275 at 2413.353 kb on - strand at 2413.360 kb on + strand at 2413.360 kb on + strand at 2413.361 kb on - strand at 2413.361 kb on - strand at 2413.368 kb on + strand at 2413.368 kb on + strand at 2413.368 kb on + strand at 2413.368 kb on + strand at 2413.369 kb on - strand at 2413.609 kb on + strand at 2413.610 kb on - strand at 2413.620 kb on + strand at 2413.621 kb on - strand at 2413.621 kb on - strand at 2413.621 kb on - strand at 2413.867 kb on + strand, within RR42_RS11280 at 2413.966 kb on + strand, within RR42_RS11280 at 2414.096 kb on - strand at 2414.154 kb on - strand at 2414.172 kb on - strand at 2414.172 kb on - strand at 2414.497 kb on + strand, within RR42_RS11285 at 2414.535 kb on - strand, within RR42_RS11285 at 2414.565 kb on + strand, within RR42_RS11285 at 2414.565 kb on + strand, within RR42_RS11285 at 2414.566 kb on - strand, within RR42_RS11285 at 2414.566 kb on - strand, within RR42_RS11285 at 2414.566 kb on - strand, within RR42_RS11285 at 2414.728 kb on - strand at 2414.728 kb on - strand at 2414.830 kb on + strand, within RR42_RS11290 at 2414.830 kb on + strand, within RR42_RS11290 at 2414.831 kb on - strand, within RR42_RS11290 at 2414.890 kb on + strand, within RR42_RS11290 at 2414.891 kb on - strand, within RR42_RS11290 at 2414.891 kb on - strand, within RR42_RS11290 at 2414.896 kb on + strand, within RR42_RS11290 at 2414.896 kb on + strand, within RR42_RS11290 at 2414.897 kb on - strand, within RR42_RS11290 at 2414.897 kb on - strand, within RR42_RS11290 at 2414.897 kb on - strand, within RR42_RS11290 at 2414.941 kb on + strand, within RR42_RS11290 at 2414.942 kb on - strand, within RR42_RS11290 at 2414.942 kb on - strand, within RR42_RS11290 at 2414.942 kb on - strand, within RR42_RS11290 at 2414.979 kb on - strand, within RR42_RS11290 at 2415.019 kb on + strand, within RR42_RS11290 at 2415.019 kb on + strand, within RR42_RS11290 at 2415.020 kb on - strand, within RR42_RS11290 at 2415.020 kb on - strand, within RR42_RS11290 at 2415.058 kb on + strand, within RR42_RS11290 at 2415.059 kb on - strand, within RR42_RS11290
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 2,412,886 + RR42_RS11275 0.67 +0.2 2,412,887 - RR42_RS11275 0.67 -0.4 2,412,887 - RR42_RS11275 0.67 -1.7 2,412,950 + RR42_RS11275 0.72 +0.2 2,412,951 - RR42_RS11275 0.72 +0.8 2,412,951 - RR42_RS11275 0.72 -0.5 2,412,951 - RR42_RS11275 0.72 +0.7 2,413,353 - -0.3 2,413,360 + -0.4 2,413,360 + -0.1 2,413,361 - -0.6 2,413,361 - -0.2 2,413,368 + -1.7 2,413,368 + -0.2 2,413,368 + +0.1 2,413,368 + +0.1 2,413,369 - -0.0 2,413,609 + +0.2 2,413,610 - +0.1 2,413,620 + +0.0 2,413,621 - +0.5 2,413,621 - +0.6 2,413,621 - +0.2 2,413,867 + RR42_RS11280 0.48 -1.1 2,413,966 + RR42_RS11280 0.71 -1.5 2,414,096 - +1.3 2,414,154 - -0.4 2,414,172 - -0.1 2,414,172 - -0.8 2,414,497 + RR42_RS11285 0.67 -0.1 2,414,535 - RR42_RS11285 0.76 -0.0 2,414,565 + RR42_RS11285 0.83 +0.2 2,414,565 + RR42_RS11285 0.83 -0.1 2,414,566 - RR42_RS11285 0.83 -0.4 2,414,566 - RR42_RS11285 0.83 +0.4 2,414,566 - RR42_RS11285 0.83 +0.8 2,414,728 - -0.2 2,414,728 - -0.4 2,414,830 + RR42_RS11290 0.11 -0.5 2,414,830 + RR42_RS11290 0.11 +0.4 2,414,831 - RR42_RS11290 0.12 +1.9 2,414,890 + RR42_RS11290 0.21 +0.2 2,414,891 - RR42_RS11290 0.22 -0.5 2,414,891 - RR42_RS11290 0.22 +0.3 2,414,896 + RR42_RS11290 0.22 -0.1 2,414,896 + RR42_RS11290 0.22 -0.2 2,414,897 - RR42_RS11290 0.23 +0.6 2,414,897 - RR42_RS11290 0.23 -1.1 2,414,897 - RR42_RS11290 0.23 +0.4 2,414,941 + RR42_RS11290 0.30 -0.0 2,414,942 - RR42_RS11290 0.30 -0.9 2,414,942 - RR42_RS11290 0.30 -0.3 2,414,942 - RR42_RS11290 0.30 -0.3 2,414,979 - RR42_RS11290 0.36 +0.7 2,415,019 + RR42_RS11290 0.43 +0.5 2,415,019 + RR42_RS11290 0.43 -0.9 2,415,020 - RR42_RS11290 0.43 +0.6 2,415,020 - RR42_RS11290 0.43 -0.2 2,415,058 + RR42_RS11290 0.49 -0.5 2,415,059 - RR42_RS11290 0.49 -0.5
Or see this region's nucleotide sequence