Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11110 and RR42_RS11115 are separated by 28 nucleotides RR42_RS11115 and RR42_RS11120 overlap by 11 nucleotides RR42_RS11120 and RR42_RS11125 are separated by 230 nucleotides
RR42_RS11110: RR42_RS11110 - serine dehydratase, at 2,373,021 to 2,373,983
_RS11110
RR42_RS11115: RR42_RS11115 - ornithine cyclodeaminase, at 2,374,012 to 2,374,983
_RS11115
RR42_RS11120: RR42_RS11120 - DNA-binding protein, at 2,374,973 to 2,375,614
_RS11120
RR42_RS11125: RR42_RS11125 - glycerophosphodiester phosphodiesterase, at 2,375,845 to 2,377,218
_RS11125
Position (kb)
2374
2375
2376 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2374.387 kb on + strand, within RR42_RS11115 at 2374.388 kb on - strand, within RR42_RS11115 at 2374.388 kb on - strand, within RR42_RS11115 at 2374.388 kb on - strand, within RR42_RS11115 at 2374.497 kb on - strand, within RR42_RS11115 at 2374.497 kb on - strand, within RR42_RS11115 at 2374.879 kb on - strand, within RR42_RS11115 at 2374.879 kb on - strand, within RR42_RS11115 at 2374.983 kb on + strand at 2374.984 kb on - strand at 2374.987 kb on + strand at 2374.987 kb on + strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.989 kb on + strand at 2374.989 kb on + strand at 2374.990 kb on - strand at 2375.293 kb on - strand, within RR42_RS11120 at 2375.364 kb on + strand, within RR42_RS11120 at 2375.365 kb on - strand, within RR42_RS11120 at 2375.386 kb on - strand, within RR42_RS11120 at 2375.603 kb on - strand at 2375.603 kb on - strand at 2375.704 kb on + strand at 2375.705 kb on - strand at 2375.705 kb on - strand at 2375.705 kb on - strand at 2375.843 kb on - strand at 2375.843 kb on - strand at 2375.989 kb on + strand, within RR42_RS11125 at 2375.990 kb on - strand, within RR42_RS11125 at 2375.990 kb on - strand, within RR42_RS11125 at 2375.992 kb on - strand, within RR42_RS11125 at 2375.992 kb on - strand, within RR42_RS11125 at 2376.250 kb on + strand, within RR42_RS11125 at 2376.250 kb on + strand, within RR42_RS11125 at 2376.251 kb on - strand, within RR42_RS11125 at 2376.251 kb on - strand, within RR42_RS11125 at 2376.253 kb on + strand, within RR42_RS11125 at 2376.361 kb on + strand, within RR42_RS11125 at 2376.362 kb on - strand, within RR42_RS11125 at 2376.362 kb on - strand, within RR42_RS11125
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 2,374,387 + RR42_RS11115 0.39 -0.1 2,374,388 - RR42_RS11115 0.39 -0.2 2,374,388 - RR42_RS11115 0.39 -1.5 2,374,388 - RR42_RS11115 0.39 -2.5 2,374,497 - RR42_RS11115 0.50 +0.0 2,374,497 - RR42_RS11115 0.50 -0.5 2,374,879 - RR42_RS11115 0.89 -2.8 2,374,879 - RR42_RS11115 0.89 -1.3 2,374,983 + +0.2 2,374,984 - +0.5 2,374,987 + -0.3 2,374,987 + +0.9 2,374,988 - +0.8 2,374,988 - -0.3 2,374,988 - +0.8 2,374,988 - -0.9 2,374,988 - -0.3 2,374,989 + -1.3 2,374,989 + -1.2 2,374,990 - -0.9 2,375,293 - RR42_RS11120 0.50 -0.7 2,375,364 + RR42_RS11120 0.61 -0.9 2,375,365 - RR42_RS11120 0.61 -2.2 2,375,386 - RR42_RS11120 0.64 -0.9 2,375,603 - -0.2 2,375,603 - -1.2 2,375,704 + -3.3 2,375,705 - -0.4 2,375,705 - -0.5 2,375,705 - +1.1 2,375,843 - +0.1 2,375,843 - -0.8 2,375,989 + RR42_RS11125 0.10 -0.4 2,375,990 - RR42_RS11125 0.11 -0.5 2,375,990 - RR42_RS11125 0.11 +1.0 2,375,992 - RR42_RS11125 0.11 -0.5 2,375,992 - RR42_RS11125 0.11 -0.5 2,376,250 + RR42_RS11125 0.29 +0.5 2,376,250 + RR42_RS11125 0.29 -0.3 2,376,251 - RR42_RS11125 0.30 +0.3 2,376,251 - RR42_RS11125 0.30 -2.1 2,376,253 + RR42_RS11125 0.30 +1.3 2,376,361 + RR42_RS11125 0.38 -0.3 2,376,362 - RR42_RS11125 0.38 -0.1 2,376,362 - RR42_RS11125 0.38 -0.3
Or see this region's nucleotide sequence