Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08025 and RR42_RS08030 are separated by 35 nucleotides RR42_RS08030 and RR42_RS08035 are separated by 2 nucleotides RR42_RS08035 and RR42_RS08040 are separated by 113 nucleotides RR42_RS08040 and RR42_RS08045 are separated by 26 nucleotides
RR42_RS08025: RR42_RS08025 - phage Gp37Gp68, at 1,720,951 to 1,721,838
_RS08025
RR42_RS08030: RR42_RS08030 - hypothetical protein, at 1,721,874 to 1,722,170
_RS08030
RR42_RS08035: RR42_RS08035 - DNA methyltransferase, at 1,722,173 to 1,723,957
_RS08035
RR42_RS08040: RR42_RS08040 - hypothetical protein, at 1,724,071 to 1,724,271
_RS08040
RR42_RS08045: RR42_RS08045 - hypothetical protein, at 1,724,298 to 1,724,849
_RS08045
Position (kb) 1722
1723
1724 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 1721.399 kb on + strand, within RR42_RS08025 at 1721.616 kb on + strand, within RR42_RS08025 at 1721.971 kb on + strand, within RR42_RS08030 at 1722.105 kb on + strand, within RR42_RS08030 at 1722.256 kb on + strand at 1722.256 kb on + strand at 1722.384 kb on + strand, within RR42_RS08035 at 1722.963 kb on + strand, within RR42_RS08035 at 1723.077 kb on + strand, within RR42_RS08035 at 1723.308 kb on + strand, within RR42_RS08035 at 1723.308 kb on + strand, within RR42_RS08035 at 1723.309 kb on - strand, within RR42_RS08035 at 1723.747 kb on - strand, within RR42_RS08035 at 1724.008 kb on + strand at 1724.034 kb on - strand at 1724.050 kb on + strand at 1724.070 kb on + strand at 1724.070 kb on + strand at 1724.138 kb on - strand, within RR42_RS08040 at 1724.167 kb on + strand, within RR42_RS08040 at 1724.170 kb on + strand, within RR42_RS08040 at 1724.227 kb on + strand, within RR42_RS08040 at 1724.228 kb on - strand, within RR42_RS08040 at 1724.267 kb on - strand at 1724.280 kb on + strand at 1724.280 kb on + strand at 1724.371 kb on - strand, within RR42_RS08045 at 1724.572 kb on + strand, within RR42_RS08045 at 1724.572 kb on + strand, within RR42_RS08045 at 1724.572 kb on + strand, within RR42_RS08045 at 1724.675 kb on - strand, within RR42_RS08045 at 1724.722 kb on + strand, within RR42_RS08045 at 1724.722 kb on + strand, within RR42_RS08045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 1,721,399 + RR42_RS08025 0.50 +1.8 1,721,616 + RR42_RS08025 0.75 +0.0 1,721,971 + RR42_RS08030 0.33 -1.4 1,722,105 + RR42_RS08030 0.78 -0.4 1,722,256 + -0.1 1,722,256 + +0.1 1,722,384 + RR42_RS08035 0.12 -1.5 1,722,963 + RR42_RS08035 0.44 +1.2 1,723,077 + RR42_RS08035 0.51 -0.3 1,723,308 + RR42_RS08035 0.64 +0.4 1,723,308 + RR42_RS08035 0.64 -0.6 1,723,309 - RR42_RS08035 0.64 -5.8 1,723,747 - RR42_RS08035 0.88 -2.3 1,724,008 + -1.4 1,724,034 - -2.5 1,724,050 + +0.5 1,724,070 + +0.8 1,724,070 + +0.1 1,724,138 - RR42_RS08040 0.33 -4.0 1,724,167 + RR42_RS08040 0.48 -0.7 1,724,170 + RR42_RS08040 0.49 -0.6 1,724,227 + RR42_RS08040 0.78 -0.1 1,724,228 - RR42_RS08040 0.78 -1.4 1,724,267 - -1.7 1,724,280 + -0.2 1,724,280 + +0.3 1,724,371 - RR42_RS08045 0.13 -0.0 1,724,572 + RR42_RS08045 0.50 -0.2 1,724,572 + RR42_RS08045 0.50 -0.4 1,724,572 + RR42_RS08045 0.50 +0.7 1,724,675 - RR42_RS08045 0.68 -3.6 1,724,722 + RR42_RS08045 0.77 +0.3 1,724,722 + RR42_RS08045 0.77 +0.6
Or see this region's nucleotide sequence