Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07490 and RR42_RS07495 are separated by 61 nucleotides RR42_RS07495 and RR42_RS07500 are separated by 212 nucleotides RR42_RS07500 and RR42_RS07505 are separated by 84 nucleotides
RR42_RS07490: RR42_RS07490 - phosphoanhydride phosphorylase, at 1,599,761 to 1,600,411
_RS07490
RR42_RS07495: RR42_RS07495 - XRE family transcriptional regulator, at 1,600,473 to 1,600,862
_RS07495
RR42_RS07500: RR42_RS07500 - benzoyl-CoA oxygenase, at 1,601,075 to 1,602,310
_RS07500
RR42_RS07505: RR42_RS07505 - benzoyl-CoA oxygenase, at 1,602,395 to 1,603,819
_RS07505
Position (kb)
1601
1602
1603 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1600.192 kb on - strand, within RR42_RS07490 at 1600.219 kb on + strand, within RR42_RS07490 at 1600.219 kb on + strand, within RR42_RS07490 at 1600.233 kb on + strand, within RR42_RS07490 at 1600.233 kb on + strand, within RR42_RS07490 at 1600.233 kb on + strand, within RR42_RS07490 at 1600.233 kb on + strand, within RR42_RS07490 at 1600.234 kb on - strand, within RR42_RS07490 at 1600.234 kb on - strand, within RR42_RS07490 at 1600.234 kb on - strand, within RR42_RS07490 at 1600.291 kb on - strand at 1600.315 kb on - strand, within RR42_RS07490 at 1600.315 kb on - strand, within RR42_RS07490 at 1600.315 kb on - strand, within RR42_RS07490 at 1600.418 kb on + strand at 1600.502 kb on - strand at 1600.516 kb on - strand, within RR42_RS07495 at 1600.516 kb on - strand, within RR42_RS07495 at 1600.690 kb on + strand, within RR42_RS07495 at 1600.691 kb on - strand, within RR42_RS07495 at 1600.691 kb on - strand, within RR42_RS07495 at 1600.892 kb on - strand at 1600.892 kb on - strand at 1600.894 kb on - strand at 1600.894 kb on - strand at 1600.934 kb on + strand at 1600.985 kb on + strand at 1600.987 kb on + strand at 1600.988 kb on - strand at 1600.988 kb on - strand at 1600.988 kb on - strand at 1601.060 kb on - strand at 1601.079 kb on + strand at 1601.079 kb on + strand at 1601.079 kb on + strand at 1601.080 kb on - strand at 1601.080 kb on - strand at 1601.080 kb on - strand at 1601.089 kb on + strand at 1601.089 kb on + strand at 1601.090 kb on - strand at 1601.211 kb on + strand, within RR42_RS07500 at 1601.211 kb on + strand, within RR42_RS07500 at 1601.211 kb on + strand at 1601.211 kb on + strand, within RR42_RS07500 at 1601.211 kb on + strand, within RR42_RS07500 at 1601.211 kb on + strand, within RR42_RS07500 at 1601.211 kb on + strand, within RR42_RS07500 at 1601.212 kb on - strand, within RR42_RS07500 at 1601.212 kb on - strand, within RR42_RS07500 at 1601.212 kb on - strand, within RR42_RS07500 at 1601.212 kb on - strand, within RR42_RS07500 at 1601.212 kb on - strand, within RR42_RS07500 at 1601.212 kb on - strand, within RR42_RS07500 at 1601.279 kb on + strand, within RR42_RS07500 at 1601.279 kb on + strand, within RR42_RS07500 at 1601.279 kb on + strand, within RR42_RS07500 at 1601.279 kb on + strand, within RR42_RS07500 at 1601.280 kb on - strand, within RR42_RS07500 at 1601.280 kb on - strand, within RR42_RS07500 at 1601.280 kb on - strand, within RR42_RS07500 at 1601.368 kb on - strand, within RR42_RS07500 at 1601.368 kb on - strand, within RR42_RS07500 at 1601.667 kb on + strand, within RR42_RS07500 at 1601.719 kb on - strand, within RR42_RS07500 at 1601.719 kb on - strand, within RR42_RS07500 at 1601.735 kb on - strand, within RR42_RS07500 at 1601.856 kb on + strand, within RR42_RS07500 at 1601.857 kb on - strand, within RR42_RS07500 at 1601.911 kb on - strand, within RR42_RS07500 at 1601.947 kb on + strand, within RR42_RS07500 at 1601.948 kb on - strand, within RR42_RS07500 at 1602.008 kb on - strand, within RR42_RS07500 at 1602.071 kb on + strand, within RR42_RS07500 at 1602.071 kb on + strand, within RR42_RS07500 at 1602.071 kb on + strand, within RR42_RS07500 at 1602.093 kb on + strand, within RR42_RS07500 at 1602.195 kb on + strand at 1602.195 kb on + strand at 1602.195 kb on + strand at 1602.195 kb on + strand at 1602.195 kb on + strand at 1602.196 kb on - strand at 1602.411 kb on + strand at 1602.412 kb on - strand at 1602.472 kb on - strand at 1602.727 kb on - strand, within RR42_RS07505 at 1602.813 kb on + strand, within RR42_RS07505 at 1602.814 kb on - strand, within RR42_RS07505 at 1602.885 kb on + strand, within RR42_RS07505 at 1602.886 kb on - strand, within RR42_RS07505 at 1602.957 kb on + strand, within RR42_RS07505 at 1602.957 kb on + strand, within RR42_RS07505 at 1602.958 kb on - strand, within RR42_RS07505 at 1602.971 kb on - strand, within RR42_RS07505 at 1603.046 kb on - strand, within RR42_RS07505
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 1,600,192 - RR42_RS07490 0.66 +0.8 1,600,219 + RR42_RS07490 0.70 -1.1 1,600,219 + RR42_RS07490 0.70 -0.7 1,600,233 + RR42_RS07490 0.73 -0.1 1,600,233 + RR42_RS07490 0.73 +1.1 1,600,233 + RR42_RS07490 0.73 -0.2 1,600,233 + RR42_RS07490 0.73 -3.9 1,600,234 - RR42_RS07490 0.73 +0.6 1,600,234 - RR42_RS07490 0.73 -0.3 1,600,234 - RR42_RS07490 0.73 +0.7 1,600,291 - +1.4 1,600,315 - RR42_RS07490 0.85 +0.3 1,600,315 - RR42_RS07490 0.85 +1.9 1,600,315 - RR42_RS07490 0.85 +0.4 1,600,418 + +1.0 1,600,502 - -0.4 1,600,516 - RR42_RS07495 0.11 -0.1 1,600,516 - RR42_RS07495 0.11 +0.3 1,600,690 + RR42_RS07495 0.56 -0.6 1,600,691 - RR42_RS07495 0.56 -0.2 1,600,691 - RR42_RS07495 0.56 +0.1 1,600,892 - -0.3 1,600,892 - +0.5 1,600,894 - -0.6 1,600,894 - -0.7 1,600,934 + +0.3 1,600,985 + -1.0 1,600,987 + +0.0 1,600,988 - -1.1 1,600,988 - +1.6 1,600,988 - -1.2 1,601,060 - +0.3 1,601,079 + -0.2 1,601,079 + +1.2 1,601,079 + +1.9 1,601,080 - -0.6 1,601,080 - -0.8 1,601,080 - +0.7 1,601,089 + -0.1 1,601,089 + -1.3 1,601,090 - -0.3 1,601,211 + RR42_RS07500 0.11 -0.8 1,601,211 + RR42_RS07500 0.11 -0.3 1,601,211 + +1.4 1,601,211 + RR42_RS07500 0.11 -0.2 1,601,211 + RR42_RS07500 0.11 +0.2 1,601,211 + RR42_RS07500 0.11 +0.4 1,601,211 + RR42_RS07500 0.11 -0.8 1,601,212 - RR42_RS07500 0.11 -2.8 1,601,212 - RR42_RS07500 0.11 +0.1 1,601,212 - RR42_RS07500 0.11 +0.8 1,601,212 - RR42_RS07500 0.11 +0.2 1,601,212 - RR42_RS07500 0.11 -0.8 1,601,212 - RR42_RS07500 0.11 +0.1 1,601,279 + RR42_RS07500 0.17 -0.7 1,601,279 + RR42_RS07500 0.17 +1.7 1,601,279 + RR42_RS07500 0.17 +0.0 1,601,279 + RR42_RS07500 0.17 +0.8 1,601,280 - RR42_RS07500 0.17 +0.9 1,601,280 - RR42_RS07500 0.17 +1.1 1,601,280 - RR42_RS07500 0.17 +0.1 1,601,368 - RR42_RS07500 0.24 -0.2 1,601,368 - RR42_RS07500 0.24 -0.5 1,601,667 + RR42_RS07500 0.48 -0.3 1,601,719 - RR42_RS07500 0.52 -0.1 1,601,719 - RR42_RS07500 0.52 +1.1 1,601,735 - RR42_RS07500 0.53 -0.3 1,601,856 + RR42_RS07500 0.63 +0.6 1,601,857 - RR42_RS07500 0.63 +0.7 1,601,911 - RR42_RS07500 0.68 +0.3 1,601,947 + RR42_RS07500 0.71 +0.1 1,601,948 - RR42_RS07500 0.71 +0.4 1,602,008 - RR42_RS07500 0.75 +0.6 1,602,071 + RR42_RS07500 0.81 +1.1 1,602,071 + RR42_RS07500 0.81 +0.0 1,602,071 + RR42_RS07500 0.81 +1.2 1,602,093 + RR42_RS07500 0.82 +0.3 1,602,195 + +0.2 1,602,195 + +0.8 1,602,195 + +0.4 1,602,195 + -0.2 1,602,195 + +0.7 1,602,196 - -1.7 1,602,411 + +0.1 1,602,412 - +0.6 1,602,472 - -2.5 1,602,727 - RR42_RS07505 0.23 +0.2 1,602,813 + RR42_RS07505 0.29 +0.5 1,602,814 - RR42_RS07505 0.29 -0.2 1,602,885 + RR42_RS07505 0.34 +0.8 1,602,886 - RR42_RS07505 0.34 +1.9 1,602,957 + RR42_RS07505 0.39 -0.0 1,602,957 + RR42_RS07505 0.39 -1.9 1,602,958 - RR42_RS07505 0.40 +0.1 1,602,971 - RR42_RS07505 0.40 +0.1 1,603,046 - RR42_RS07505 0.46 -0.1
Or see this region's nucleotide sequence