Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS05370

Experiment: R2A with nitrate 150 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS05360 and RR42_RS05365 overlap by 16 nucleotidesRR42_RS05365 and RR42_RS05370 overlap by 2 nucleotidesRR42_RS05370 and RR42_RS05375 are separated by 24 nucleotidesRR42_RS05375 and RR42_RS05380 are separated by 38 nucleotides RR42_RS05360: RR42_RS05360 - membrane protein, at 1,186,341 to 1,186,958 _RS05360 RR42_RS05365: RR42_RS05365 - hypothetical protein, at 1,186,943 to 1,187,275 _RS05365 RR42_RS05370: RR42_RS05370 - ABC transporter permease, at 1,187,274 to 1,189,817 _RS05370 RR42_RS05375: RR42_RS05375 - hemoglobin-like protein, at 1,189,842 to 1,190,279 _RS05375 RR42_RS05380: RR42_RS05380 - membrane protein, at 1,190,318 to 1,190,935 _RS05380 Position (kb) 1187 1188 1189 1190Strain fitness (log2 ratio) -3 -2 -1 0 1at 1186.280 kb on + strandat 1186.284 kb on - strandat 1186.296 kb on + strandat 1186.345 kb on + strandat 1186.346 kb on - strandat 1186.346 kb on - strandat 1186.346 kb on - strandat 1186.399 kb on - strandat 1186.399 kb on - strandat 1186.399 kb on - strandat 1186.890 kb on + strand, within RR42_RS05360at 1186.891 kb on - strand, within RR42_RS05360at 1187.119 kb on + strand, within RR42_RS05365at 1187.120 kb on - strand, within RR42_RS05365at 1187.120 kb on - strand, within RR42_RS05365at 1187.120 kb on - strand, within RR42_RS05365at 1187.132 kb on + strand, within RR42_RS05365at 1187.132 kb on + strand, within RR42_RS05365at 1187.133 kb on - strand, within RR42_RS05365at 1187.133 kb on - strand, within RR42_RS05365at 1187.929 kb on - strand, within RR42_RS05370at 1188.153 kb on + strand, within RR42_RS05370at 1188.153 kb on + strand, within RR42_RS05370at 1188.153 kb on + strand, within RR42_RS05370at 1188.154 kb on - strand, within RR42_RS05370at 1188.155 kb on + strand, within RR42_RS05370at 1188.155 kb on + strand, within RR42_RS05370at 1188.301 kb on - strand, within RR42_RS05370at 1188.301 kb on - strand, within RR42_RS05370at 1188.634 kb on + strand, within RR42_RS05370at 1188.635 kb on - strand, within RR42_RS05370at 1188.635 kb on - strand, within RR42_RS05370at 1188.864 kb on - strand, within RR42_RS05370at 1188.959 kb on + strand, within RR42_RS05370at 1188.959 kb on + strand, within RR42_RS05370at 1188.959 kb on + strand, within RR42_RS05370at 1188.960 kb on - strand, within RR42_RS05370at 1188.960 kb on - strand, within RR42_RS05370at 1188.960 kb on - strand, within RR42_RS05370at 1189.041 kb on + strand, within RR42_RS05370at 1189.041 kb on + strand, within RR42_RS05370at 1189.043 kb on + strand, within RR42_RS05370at 1189.044 kb on - strand, within RR42_RS05370at 1189.302 kb on + strand, within RR42_RS05370at 1189.302 kb on + strand, within RR42_RS05370at 1189.302 kb on + strand, within RR42_RS05370at 1189.303 kb on - strand, within RR42_RS05370at 1189.303 kb on - strand, within RR42_RS05370at 1189.305 kb on - strand, within RR42_RS05370at 1189.305 kb on - strand, within RR42_RS05370at 1189.491 kb on + strand, within RR42_RS05370at 1189.491 kb on + strand, within RR42_RS05370at 1189.491 kb on + strand, within RR42_RS05370at 1189.492 kb on - strand, within RR42_RS05370at 1189.492 kb on - strand, within RR42_RS05370at 1189.588 kb on - strandat 1189.841 kb on + strandat 1189.841 kb on + strandat 1189.841 kb on + strandat 1189.841 kb on + strandat 1189.842 kb on - strandat 1189.842 kb on - strandat 1189.912 kb on - strand, within RR42_RS05375at 1189.962 kb on + strand, within RR42_RS05375at 1189.962 kb on + strand, within RR42_RS05375at 1189.963 kb on - strand, within RR42_RS05375at 1189.963 kb on - strand, within RR42_RS05375at 1190.304 kb on + strandat 1190.305 kb on - strandat 1190.305 kb on - strandat 1190.305 kb on - strandat 1190.305 kb on - strandat 1190.374 kb on - strandat 1190.705 kb on + strand, within RR42_RS05380

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM
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1,186,280 + +0.4
1,186,284 - +1.1
1,186,296 + -1.0
1,186,345 + -0.1
1,186,346 - -0.7
1,186,346 - +1.0
1,186,346 - +1.2
1,186,399 - +0.4
1,186,399 - +0.5
1,186,399 - +0.4
1,186,890 + RR42_RS05360 0.89 -0.4
1,186,891 - RR42_RS05360 0.89 +0.5
1,187,119 + RR42_RS05365 0.53 -0.6
1,187,120 - RR42_RS05365 0.53 +0.7
1,187,120 - RR42_RS05365 0.53 -2.2
1,187,120 - RR42_RS05365 0.53 +0.1
1,187,132 + RR42_RS05365 0.57 +1.6
1,187,132 + RR42_RS05365 0.57 +0.0
1,187,133 - RR42_RS05365 0.57 -0.2
1,187,133 - RR42_RS05365 0.57 +0.1
1,187,929 - RR42_RS05370 0.26 +0.0
1,188,153 + RR42_RS05370 0.35 -0.1
1,188,153 + RR42_RS05370 0.35 +0.9
1,188,153 + RR42_RS05370 0.35 +0.1
1,188,154 - RR42_RS05370 0.35 +1.3
1,188,155 + RR42_RS05370 0.35 -1.4
1,188,155 + RR42_RS05370 0.35 +0.5
1,188,301 - RR42_RS05370 0.40 -0.1
1,188,301 - RR42_RS05370 0.40 +0.1
1,188,634 + RR42_RS05370 0.53 +0.2
1,188,635 - RR42_RS05370 0.53 +1.3
1,188,635 - RR42_RS05370 0.53 +1.0
1,188,864 - RR42_RS05370 0.62 +0.8
1,188,959 + RR42_RS05370 0.66 +0.7
1,188,959 + RR42_RS05370 0.66 +0.7
1,188,959 + RR42_RS05370 0.66 +0.3
1,188,960 - RR42_RS05370 0.66 +0.7
1,188,960 - RR42_RS05370 0.66 -0.6
1,188,960 - RR42_RS05370 0.66 +0.3
1,189,041 + RR42_RS05370 0.69 -0.5
1,189,041 + RR42_RS05370 0.69 -1.0
1,189,043 + RR42_RS05370 0.70 -0.4
1,189,044 - RR42_RS05370 0.70 +1.0
1,189,302 + RR42_RS05370 0.80 +0.6
1,189,302 + RR42_RS05370 0.80 -0.6
1,189,302 + RR42_RS05370 0.80 +0.4
1,189,303 - RR42_RS05370 0.80 -0.6
1,189,303 - RR42_RS05370 0.80 -0.3
1,189,305 - RR42_RS05370 0.80 +0.9
1,189,305 - RR42_RS05370 0.80 -1.1
1,189,491 + RR42_RS05370 0.87 +0.0
1,189,491 + RR42_RS05370 0.87 +1.1
1,189,491 + RR42_RS05370 0.87 -1.0
1,189,492 - RR42_RS05370 0.87 +0.1
1,189,492 - RR42_RS05370 0.87 +0.2
1,189,588 - -2.0
1,189,841 + +0.2
1,189,841 + +0.1
1,189,841 + +0.1
1,189,841 + +0.5
1,189,842 - +1.0
1,189,842 - +0.1
1,189,912 - RR42_RS05375 0.16 -3.1
1,189,962 + RR42_RS05375 0.27 -0.9
1,189,962 + RR42_RS05375 0.27 -0.5
1,189,963 - RR42_RS05375 0.28 +1.0
1,189,963 - RR42_RS05375 0.28 -0.1
1,190,304 + -0.4
1,190,305 - -0.1
1,190,305 - +0.6
1,190,305 - -0.1
1,190,305 - +1.3
1,190,374 - -0.2
1,190,705 + RR42_RS05380 0.63 +1.0

Or see this region's nucleotide sequence