Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04880 and RR42_RS04885 overlap by 4 nucleotides RR42_RS04885 and RR42_RS04890 are separated by 13 nucleotides RR42_RS04890 and RR42_RS04895 are separated by 139 nucleotides
RR42_RS04880: RR42_RS04880 - coproporphyrinogen III oxidase, at 1,082,827 to 1,084,077
_RS04880
RR42_RS04885: RR42_RS04885 - nucleoside-triphosphate diphosphatase, at 1,084,074 to 1,084,691
_RS04885
RR42_RS04890: RR42_RS04890 - ribonuclease PH, at 1,084,705 to 1,085,424
_RS04890
RR42_RS04895: RR42_RS04895 - serine/threonine protein phosphatase, at 1,085,564 to 1,086,478
_RS04895
Position (kb)
1084
1085
1086 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1083.809 kb on - strand, within RR42_RS04880 at 1083.854 kb on + strand, within RR42_RS04880 at 1083.920 kb on + strand, within RR42_RS04880 at 1083.949 kb on - strand, within RR42_RS04880 at 1083.957 kb on - strand at 1084.222 kb on + strand, within RR42_RS04885 at 1084.333 kb on + strand, within RR42_RS04885 at 1084.333 kb on + strand, within RR42_RS04885 at 1084.334 kb on - strand, within RR42_RS04885 at 1084.334 kb on - strand, within RR42_RS04885 at 1084.336 kb on + strand, within RR42_RS04885 at 1084.336 kb on + strand, within RR42_RS04885 at 1084.337 kb on - strand, within RR42_RS04885 at 1084.337 kb on - strand, within RR42_RS04885 at 1084.337 kb on - strand, within RR42_RS04885 at 1084.337 kb on - strand, within RR42_RS04885 at 1084.337 kb on - strand, within RR42_RS04885 at 1084.339 kb on + strand, within RR42_RS04885 at 1084.340 kb on - strand, within RR42_RS04885 at 1084.340 kb on - strand, within RR42_RS04885 at 1084.442 kb on - strand, within RR42_RS04885 at 1084.704 kb on - strand at 1084.895 kb on + strand, within RR42_RS04890 at 1084.896 kb on - strand, within RR42_RS04890 at 1085.238 kb on - strand, within RR42_RS04890 at 1085.366 kb on + strand at 1085.366 kb on + strand at 1085.367 kb on - strand at 1085.385 kb on + strand at 1085.385 kb on + strand at 1085.906 kb on + strand, within RR42_RS04895 at 1085.907 kb on - strand, within RR42_RS04895 at 1085.907 kb on - strand, within RR42_RS04895 at 1085.907 kb on - strand, within RR42_RS04895 at 1085.988 kb on + strand, within RR42_RS04895 at 1085.988 kb on + strand, within RR42_RS04895 at 1085.988 kb on + strand, within RR42_RS04895 at 1085.988 kb on + strand, within RR42_RS04895 at 1085.988 kb on + strand, within RR42_RS04895 at 1085.988 kb on + strand, within RR42_RS04895 at 1085.988 kb on + strand, within RR42_RS04895 at 1085.989 kb on - strand, within RR42_RS04895 at 1085.989 kb on - strand, within RR42_RS04895 at 1085.989 kb on - strand, within RR42_RS04895 at 1085.989 kb on - strand, within RR42_RS04895 at 1085.989 kb on - strand, within RR42_RS04895 at 1086.094 kb on + strand, within RR42_RS04895 at 1086.104 kb on + strand, within RR42_RS04895 at 1086.105 kb on - strand, within RR42_RS04895 at 1086.105 kb on - strand, within RR42_RS04895 at 1086.111 kb on + strand, within RR42_RS04895 at 1086.111 kb on + strand, within RR42_RS04895 at 1086.111 kb on + strand, within RR42_RS04895 at 1086.111 kb on + strand, within RR42_RS04895 at 1086.181 kb on + strand, within RR42_RS04895 at 1086.181 kb on + strand, within RR42_RS04895 at 1086.217 kb on - strand, within RR42_RS04895 at 1086.411 kb on + strand at 1086.411 kb on + strand at 1086.411 kb on + strand at 1086.412 kb on - strand at 1086.412 kb on - strand at 1086.412 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 1,083,809 - RR42_RS04880 0.78 -1.2 1,083,854 + RR42_RS04880 0.82 +0.7 1,083,920 + RR42_RS04880 0.87 -0.3 1,083,949 - RR42_RS04880 0.90 +1.0 1,083,957 - +0.6 1,084,222 + RR42_RS04885 0.24 +0.1 1,084,333 + RR42_RS04885 0.42 -0.8 1,084,333 + RR42_RS04885 0.42 -0.4 1,084,334 - RR42_RS04885 0.42 -1.5 1,084,334 - RR42_RS04885 0.42 +0.2 1,084,336 + RR42_RS04885 0.42 +0.7 1,084,336 + RR42_RS04885 0.42 +0.8 1,084,337 - RR42_RS04885 0.43 +0.8 1,084,337 - RR42_RS04885 0.43 -0.4 1,084,337 - RR42_RS04885 0.43 +0.2 1,084,337 - RR42_RS04885 0.43 -2.1 1,084,337 - RR42_RS04885 0.43 +0.6 1,084,339 + RR42_RS04885 0.43 +1.0 1,084,340 - RR42_RS04885 0.43 -0.6 1,084,340 - RR42_RS04885 0.43 -0.7 1,084,442 - RR42_RS04885 0.60 -1.5 1,084,704 - +0.8 1,084,895 + RR42_RS04890 0.26 +1.6 1,084,896 - RR42_RS04890 0.27 +0.3 1,085,238 - RR42_RS04890 0.74 +0.1 1,085,366 + +1.1 1,085,366 + +1.2 1,085,367 - -0.5 1,085,385 + -1.4 1,085,385 + +0.4 1,085,906 + RR42_RS04895 0.37 +1.1 1,085,907 - RR42_RS04895 0.37 +0.5 1,085,907 - RR42_RS04895 0.37 +0.9 1,085,907 - RR42_RS04895 0.37 +1.3 1,085,988 + RR42_RS04895 0.46 +0.8 1,085,988 + RR42_RS04895 0.46 +1.0 1,085,988 + RR42_RS04895 0.46 -1.1 1,085,988 + RR42_RS04895 0.46 +0.8 1,085,988 + RR42_RS04895 0.46 +1.2 1,085,988 + RR42_RS04895 0.46 +0.6 1,085,988 + RR42_RS04895 0.46 +1.1 1,085,989 - RR42_RS04895 0.46 +1.2 1,085,989 - RR42_RS04895 0.46 +1.0 1,085,989 - RR42_RS04895 0.46 +1.9 1,085,989 - RR42_RS04895 0.46 +1.3 1,085,989 - RR42_RS04895 0.46 +1.1 1,086,094 + RR42_RS04895 0.58 +0.0 1,086,104 + RR42_RS04895 0.59 +1.1 1,086,105 - RR42_RS04895 0.59 +1.8 1,086,105 - RR42_RS04895 0.59 +1.0 1,086,111 + RR42_RS04895 0.60 +1.8 1,086,111 + RR42_RS04895 0.60 +1.2 1,086,111 + RR42_RS04895 0.60 +1.1 1,086,111 + RR42_RS04895 0.60 +1.4 1,086,181 + RR42_RS04895 0.67 +1.1 1,086,181 + RR42_RS04895 0.67 +1.1 1,086,217 - RR42_RS04895 0.71 +1.2 1,086,411 + +0.9 1,086,411 + +1.3 1,086,411 + +0.3 1,086,412 - +1.7 1,086,412 - +1.7 1,086,412 - +1.1
Or see this region's nucleotide sequence