Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03405 and RR42_RS03410 are separated by 16 nucleotides RR42_RS03410 and RR42_RS03415 overlap by 7 nucleotides
RR42_RS03405: RR42_RS03405 - epoxyqueuosine reductase, at 757,589 to 758,809
_RS03405
RR42_RS03410: RR42_RS03410 - ATPase, at 758,826 to 759,338
_RS03410
RR42_RS03415: RR42_RS03415 - N-acetylmuramoyl-L-alanine amidase, at 759,332 to 760,837
_RS03415
Position (kb) 758
759
760 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 757.947 kb on + strand, within RR42_RS03405 at 757.947 kb on + strand, within RR42_RS03405 at 757.947 kb on + strand, within RR42_RS03405 at 757.948 kb on - strand, within RR42_RS03405 at 758.013 kb on + strand, within RR42_RS03405 at 758.349 kb on + strand, within RR42_RS03405 at 758.452 kb on - strand, within RR42_RS03405 at 758.533 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.820 kb on + strand at 758.820 kb on + strand at 758.820 kb on + strand at 759.506 kb on + strand, within RR42_RS03415 at 759.506 kb on + strand, within RR42_RS03415 at 759.507 kb on - strand, within RR42_RS03415 at 759.656 kb on + strand, within RR42_RS03415 at 759.656 kb on + strand, within RR42_RS03415 at 759.657 kb on - strand, within RR42_RS03415 at 759.657 kb on - strand, within RR42_RS03415 at 759.745 kb on + strand, within RR42_RS03415 at 759.746 kb on - strand, within RR42_RS03415 at 759.794 kb on + strand, within RR42_RS03415 at 760.268 kb on - strand, within RR42_RS03415 at 760.268 kb on - strand, within RR42_RS03415 at 760.318 kb on - strand, within RR42_RS03415
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 757,947 + RR42_RS03405 0.29 +0.8 757,947 + RR42_RS03405 0.29 +0.2 757,947 + RR42_RS03405 0.29 +0.5 757,948 - RR42_RS03405 0.29 +0.1 758,013 + RR42_RS03405 0.35 +0.2 758,349 + RR42_RS03405 0.62 +0.7 758,452 - RR42_RS03405 0.71 +0.2 758,533 + RR42_RS03405 0.77 +1.0 758,535 + RR42_RS03405 0.77 +1.4 758,535 + RR42_RS03405 0.77 -0.2 758,535 + RR42_RS03405 0.77 +0.6 758,535 + RR42_RS03405 0.77 +0.7 758,535 + RR42_RS03405 0.77 +0.8 758,820 + +0.8 758,820 + +0.4 758,820 + -0.1 759,506 + RR42_RS03415 0.12 -1.7 759,506 + RR42_RS03415 0.12 -2.6 759,507 - RR42_RS03415 0.12 -2.8 759,656 + RR42_RS03415 0.22 -1.8 759,656 + RR42_RS03415 0.22 -1.3 759,657 - RR42_RS03415 0.22 -0.2 759,657 - RR42_RS03415 0.22 -0.4 759,745 + RR42_RS03415 0.27 -2.8 759,746 - RR42_RS03415 0.27 -2.5 759,794 + RR42_RS03415 0.31 -0.9 760,268 - RR42_RS03415 0.62 -1.5 760,268 - RR42_RS03415 0.62 -2.9 760,318 - RR42_RS03415 0.65 -0.9
Or see this region's nucleotide sequence