Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03345 and RR42_RS03350 are separated by 125 nucleotides RR42_RS03350 and RR42_RS03355 are separated by 245 nucleotides
RR42_RS03345: RR42_RS03345 - recombinase XerD, at 743,542 to 744,462
_RS03345
RR42_RS03350: RR42_RS03350 - cysteine methyltransferase, at 744,588 to 745,067
_RS03350
RR42_RS03355: RR42_RS03355 - N-acylmannosamine kinase, at 745,313 to 746,572
_RS03355
Position (kb) 744
745
746 Strain fitness (log2 ratio) -2
-1
0
1
2 at 743.604 kb on - strand at 743.861 kb on + strand, within RR42_RS03345 at 743.862 kb on - strand, within RR42_RS03345 at 743.862 kb on - strand, within RR42_RS03345 at 744.011 kb on + strand, within RR42_RS03345 at 744.011 kb on + strand, within RR42_RS03345 at 744.011 kb on + strand, within RR42_RS03345 at 744.011 kb on + strand, within RR42_RS03345 at 744.011 kb on + strand, within RR42_RS03345 at 744.011 kb on + strand, within RR42_RS03345 at 744.012 kb on - strand, within RR42_RS03345 at 744.790 kb on + strand, within RR42_RS03350 at 744.790 kb on + strand, within RR42_RS03350 at 744.790 kb on + strand, within RR42_RS03350 at 744.791 kb on - strand, within RR42_RS03350 at 744.901 kb on + strand, within RR42_RS03350 at 744.901 kb on + strand, within RR42_RS03350 at 744.902 kb on - strand, within RR42_RS03350 at 744.904 kb on + strand, within RR42_RS03350 at 744.905 kb on - strand, within RR42_RS03350 at 744.905 kb on - strand at 744.905 kb on - strand, within RR42_RS03350 at 744.980 kb on - strand, within RR42_RS03350 at 744.980 kb on - strand, within RR42_RS03350 at 745.099 kb on - strand at 745.232 kb on + strand at 745.244 kb on + strand at 745.293 kb on + strand at 745.293 kb on + strand at 745.293 kb on + strand at 745.294 kb on - strand at 745.364 kb on + strand at 745.784 kb on + strand, within RR42_RS03355 at 745.784 kb on + strand, within RR42_RS03355 at 745.785 kb on - strand, within RR42_RS03355 at 745.952 kb on + strand, within RR42_RS03355 at 745.982 kb on + strand, within RR42_RS03355 at 745.982 kb on + strand, within RR42_RS03355 at 745.982 kb on + strand, within RR42_RS03355 at 745.982 kb on + strand, within RR42_RS03355 at 745.983 kb on - strand, within RR42_RS03355 at 745.983 kb on - strand, within RR42_RS03355
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 743,604 - -0.3 743,861 + RR42_RS03345 0.35 +0.4 743,862 - RR42_RS03345 0.35 +0.3 743,862 - RR42_RS03345 0.35 +0.4 744,011 + RR42_RS03345 0.51 -2.1 744,011 + RR42_RS03345 0.51 +1.7 744,011 + RR42_RS03345 0.51 +1.0 744,011 + RR42_RS03345 0.51 -0.1 744,011 + RR42_RS03345 0.51 +0.1 744,011 + RR42_RS03345 0.51 -0.5 744,012 - RR42_RS03345 0.51 +0.5 744,790 + RR42_RS03350 0.42 +0.6 744,790 + RR42_RS03350 0.42 +0.2 744,790 + RR42_RS03350 0.42 +0.2 744,791 - RR42_RS03350 0.42 +0.5 744,901 + RR42_RS03350 0.65 -0.2 744,901 + RR42_RS03350 0.65 +0.1 744,902 - RR42_RS03350 0.65 +1.2 744,904 + RR42_RS03350 0.66 +0.9 744,905 - RR42_RS03350 0.66 +0.8 744,905 - +1.8 744,905 - RR42_RS03350 0.66 -0.8 744,980 - RR42_RS03350 0.82 +0.7 744,980 - RR42_RS03350 0.82 +0.9 745,099 - +1.2 745,232 + +0.3 745,244 + -0.7 745,293 + +0.3 745,293 + -2.6 745,293 + +0.9 745,294 - -1.7 745,364 + +0.1 745,784 + RR42_RS03355 0.37 -0.1 745,784 + RR42_RS03355 0.37 +1.0 745,785 - RR42_RS03355 0.37 -0.1 745,952 + RR42_RS03355 0.51 +0.1 745,982 + RR42_RS03355 0.53 +0.4 745,982 + RR42_RS03355 0.53 +1.3 745,982 + RR42_RS03355 0.53 +0.1 745,982 + RR42_RS03355 0.53 -0.2 745,983 - RR42_RS03355 0.53 +1.5 745,983 - RR42_RS03355 0.53 +0.0
Or see this region's nucleotide sequence