Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03145 and RR42_RS03150 are separated by 48 nucleotides RR42_RS03150 and RR42_RS03155 are separated by 77 nucleotides RR42_RS03155 and RR42_RS03160 are separated by 160 nucleotides RR42_RS03160 and RR42_RS03165 are separated by 57 nucleotides
RR42_RS03145: RR42_RS03145 - peptidase M48, at 694,642 to 696,450
_RS03145
RR42_RS03150: RR42_RS03150 - hypothetical protein, at 696,499 to 697,110
_RS03150
RR42_RS03155: RR42_RS03155 - alpha/beta hydrolase, at 697,188 to 698,174
_RS03155
RR42_RS03160: RR42_RS03160 - hypothetical protein, at 698,335 to 698,775
_RS03160
RR42_RS03165: RR42_RS03165 - ADP-heptose--LPS heptosyltransferase, at 698,833 to 699,855
_RS03165
Position (kb) 697
698
699 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 696.236 kb on - strand, within RR42_RS03145 at 696.236 kb on - strand, within RR42_RS03145 at 696.286 kb on + strand at 696.286 kb on + strand at 696.286 kb on + strand at 696.286 kb on + strand at 696.286 kb on + strand at 696.287 kb on - strand at 696.287 kb on - strand at 696.305 kb on - strand at 696.361 kb on + strand at 696.362 kb on - strand at 696.445 kb on - strand at 696.445 kb on - strand at 696.445 kb on - strand at 696.614 kb on - strand, within RR42_RS03150 at 696.663 kb on + strand, within RR42_RS03150 at 696.664 kb on - strand, within RR42_RS03150 at 696.664 kb on - strand, within RR42_RS03150 at 696.864 kb on - strand, within RR42_RS03150 at 696.864 kb on - strand, within RR42_RS03150 at 696.905 kb on + strand, within RR42_RS03150 at 697.004 kb on + strand, within RR42_RS03150 at 697.005 kb on - strand, within RR42_RS03150 at 697.055 kb on + strand at 697.066 kb on + strand at 697.168 kb on - strand at 697.168 kb on - strand at 697.239 kb on - strand at 697.277 kb on + strand at 697.277 kb on + strand at 697.278 kb on - strand at 697.370 kb on - strand, within RR42_RS03155 at 697.509 kb on - strand, within RR42_RS03155 at 697.509 kb on - strand, within RR42_RS03155 at 697.522 kb on + strand, within RR42_RS03155 at 697.627 kb on + strand, within RR42_RS03155 at 697.627 kb on + strand, within RR42_RS03155 at 697.627 kb on + strand, within RR42_RS03155 at 697.628 kb on - strand, within RR42_RS03155 at 697.807 kb on + strand, within RR42_RS03155 at 697.864 kb on + strand, within RR42_RS03155 at 697.864 kb on + strand, within RR42_RS03155 at 697.864 kb on + strand, within RR42_RS03155 at 697.864 kb on + strand, within RR42_RS03155 at 697.966 kb on + strand, within RR42_RS03155 at 698.298 kb on + strand at 698.298 kb on + strand at 698.337 kb on - strand at 698.447 kb on + strand, within RR42_RS03160 at 698.447 kb on + strand, within RR42_RS03160 at 698.447 kb on + strand, within RR42_RS03160 at 698.447 kb on + strand, within RR42_RS03160 at 698.447 kb on + strand, within RR42_RS03160 at 698.447 kb on + strand, within RR42_RS03160 at 698.448 kb on - strand, within RR42_RS03160 at 698.448 kb on - strand, within RR42_RS03160 at 698.448 kb on - strand, within RR42_RS03160 at 698.448 kb on - strand, within RR42_RS03160 at 698.462 kb on + strand, within RR42_RS03160 at 698.462 kb on + strand, within RR42_RS03160 at 698.462 kb on + strand, within RR42_RS03160 at 698.463 kb on - strand, within RR42_RS03160 at 698.463 kb on - strand, within RR42_RS03160 at 698.463 kb on - strand, within RR42_RS03160 at 698.463 kb on - strand, within RR42_RS03160 at 698.463 kb on - strand, within RR42_RS03160 at 698.538 kb on + strand, within RR42_RS03160 at 698.538 kb on + strand, within RR42_RS03160 at 698.538 kb on + strand, within RR42_RS03160 at 698.539 kb on - strand, within RR42_RS03160 at 698.539 kb on - strand, within RR42_RS03160 at 698.539 kb on - strand, within RR42_RS03160 at 698.705 kb on - strand, within RR42_RS03160 at 698.731 kb on + strand, within RR42_RS03160 at 698.731 kb on + strand, within RR42_RS03160 at 698.731 kb on + strand, within RR42_RS03160 at 698.731 kb on + strand, within RR42_RS03160 at 698.731 kb on + strand, within RR42_RS03160 at 698.731 kb on + strand, within RR42_RS03160 at 698.731 kb on + strand, within RR42_RS03160 at 698.732 kb on - strand at 698.732 kb on - strand at 698.732 kb on - strand at 698.732 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 696,236 - RR42_RS03145 0.88 -1.3 696,236 - RR42_RS03145 0.88 +0.2 696,286 + -0.5 696,286 + +0.5 696,286 + +0.2 696,286 + +0.6 696,286 + +1.1 696,287 - +0.4 696,287 - -0.3 696,305 - +0.2 696,361 + -1.2 696,362 - +0.6 696,445 - +0.0 696,445 - +0.4 696,445 - +0.4 696,614 - RR42_RS03150 0.19 -0.1 696,663 + RR42_RS03150 0.27 -0.2 696,664 - RR42_RS03150 0.27 +0.4 696,664 - RR42_RS03150 0.27 +0.3 696,864 - RR42_RS03150 0.60 +0.3 696,864 - RR42_RS03150 0.60 -0.0 696,905 + RR42_RS03150 0.66 -1.1 697,004 + RR42_RS03150 0.83 -0.2 697,005 - RR42_RS03150 0.83 -0.1 697,055 + +0.0 697,066 + +0.3 697,168 - +0.1 697,168 - +0.9 697,239 - -0.8 697,277 + -0.3 697,277 + -0.1 697,278 - -0.9 697,370 - RR42_RS03155 0.18 +0.4 697,509 - RR42_RS03155 0.33 +1.0 697,509 - RR42_RS03155 0.33 +0.5 697,522 + RR42_RS03155 0.34 +0.0 697,627 + RR42_RS03155 0.44 +0.6 697,627 + RR42_RS03155 0.44 +1.3 697,627 + RR42_RS03155 0.44 +0.1 697,628 - RR42_RS03155 0.45 -1.8 697,807 + RR42_RS03155 0.63 -0.6 697,864 + RR42_RS03155 0.68 -0.1 697,864 + RR42_RS03155 0.68 -0.4 697,864 + RR42_RS03155 0.68 +0.6 697,864 + RR42_RS03155 0.68 -0.5 697,966 + RR42_RS03155 0.79 +0.2 698,298 + +0.5 698,298 + -0.3 698,337 - +0.7 698,447 + RR42_RS03160 0.25 +0.7 698,447 + RR42_RS03160 0.25 -0.8 698,447 + RR42_RS03160 0.25 +0.4 698,447 + RR42_RS03160 0.25 -0.4 698,447 + RR42_RS03160 0.25 -0.3 698,447 + RR42_RS03160 0.25 -1.1 698,448 - RR42_RS03160 0.26 -0.6 698,448 - RR42_RS03160 0.26 +0.2 698,448 - RR42_RS03160 0.26 -0.1 698,448 - RR42_RS03160 0.26 -0.2 698,462 + RR42_RS03160 0.29 +0.8 698,462 + RR42_RS03160 0.29 +0.1 698,462 + RR42_RS03160 0.29 -0.2 698,463 - RR42_RS03160 0.29 +0.1 698,463 - RR42_RS03160 0.29 +0.4 698,463 - RR42_RS03160 0.29 +1.4 698,463 - RR42_RS03160 0.29 +0.4 698,463 - RR42_RS03160 0.29 +0.2 698,538 + RR42_RS03160 0.46 +1.0 698,538 + RR42_RS03160 0.46 +0.0 698,538 + RR42_RS03160 0.46 +0.5 698,539 - RR42_RS03160 0.46 +0.7 698,539 - RR42_RS03160 0.46 +0.2 698,539 - RR42_RS03160 0.46 +0.1 698,705 - RR42_RS03160 0.84 +0.3 698,731 + RR42_RS03160 0.90 -0.2 698,731 + RR42_RS03160 0.90 -2.8 698,731 + RR42_RS03160 0.90 -0.9 698,731 + RR42_RS03160 0.90 -0.0 698,731 + RR42_RS03160 0.90 -0.6 698,731 + RR42_RS03160 0.90 -0.1 698,731 + RR42_RS03160 0.90 -1.4 698,732 - +0.5 698,732 - -0.4 698,732 - -0.6 698,732 - -1.1
Or see this region's nucleotide sequence