Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02850 and RR42_RS02855 are separated by 40 nucleotides RR42_RS02855 and RR42_RS02860 are separated by 94 nucleotides RR42_RS02860 and RR42_RS02865 are separated by 123 nucleotides
RR42_RS02850: RR42_RS02850 - phoH-like protein, at 635,155 to 636,150
_RS02850
RR42_RS02855: RR42_RS02855 - dimethylallyladenosine tRNA methylthiotransferase, at 636,191 to 637,543
_RS02855
RR42_RS02860: RR42_RS02860 - hypothetical protein, at 637,638 to 638,018
_RS02860
RR42_RS02865: RR42_RS02865 - chloride channel protein, at 638,142 to 639,830
_RS02865
Position (kb) 636
637
638 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 635.228 kb on - strand at 635.466 kb on - strand, within RR42_RS02850 at 635.466 kb on - strand, within RR42_RS02850 at 635.550 kb on - strand, within RR42_RS02850 at 635.564 kb on + strand, within RR42_RS02850 at 635.565 kb on - strand, within RR42_RS02850 at 635.565 kb on - strand, within RR42_RS02850 at 635.699 kb on + strand, within RR42_RS02850 at 635.700 kb on - strand, within RR42_RS02850 at 635.874 kb on - strand, within RR42_RS02850 at 635.958 kb on - strand, within RR42_RS02850 at 636.272 kb on - strand at 636.272 kb on - strand at 636.536 kb on - strand, within RR42_RS02855 at 636.688 kb on - strand, within RR42_RS02855 at 636.789 kb on + strand, within RR42_RS02855 at 636.790 kb on - strand, within RR42_RS02855 at 636.790 kb on - strand, within RR42_RS02855 at 636.790 kb on - strand, within RR42_RS02855 at 636.838 kb on - strand, within RR42_RS02855 at 636.931 kb on - strand, within RR42_RS02855 at 637.005 kb on - strand, within RR42_RS02855 at 637.005 kb on - strand, within RR42_RS02855 at 637.060 kb on - strand, within RR42_RS02855 at 637.060 kb on - strand, within RR42_RS02855 at 637.069 kb on - strand, within RR42_RS02855 at 637.069 kb on - strand, within RR42_RS02855 at 637.475 kb on - strand at 637.519 kb on - strand at 637.519 kb on - strand at 637.519 kb on - strand at 637.634 kb on - strand at 637.634 kb on - strand at 637.639 kb on + strand at 637.640 kb on - strand at 637.640 kb on - strand at 637.640 kb on - strand at 637.640 kb on - strand at 637.763 kb on + strand, within RR42_RS02860 at 637.960 kb on - strand, within RR42_RS02860 at 637.960 kb on - strand, within RR42_RS02860 at 638.106 kb on + strand at 638.107 kb on - strand at 638.107 kb on - strand at 638.107 kb on - strand at 638.107 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.143 kb on + strand at 638.144 kb on - strand at 638.144 kb on - strand at 638.413 kb on + strand, within RR42_RS02865 at 638.413 kb on + strand, within RR42_RS02865 at 638.413 kb on + strand, within RR42_RS02865 at 638.413 kb on + strand, within RR42_RS02865 at 638.414 kb on - strand, within RR42_RS02865 at 638.419 kb on + strand, within RR42_RS02865
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 635,228 - +0.2 635,466 - RR42_RS02850 0.31 +0.2 635,466 - RR42_RS02850 0.31 -0.3 635,550 - RR42_RS02850 0.40 +0.9 635,564 + RR42_RS02850 0.41 +1.7 635,565 - RR42_RS02850 0.41 -0.0 635,565 - RR42_RS02850 0.41 -2.3 635,699 + RR42_RS02850 0.55 -0.2 635,700 - RR42_RS02850 0.55 -0.1 635,874 - RR42_RS02850 0.72 +1.2 635,958 - RR42_RS02850 0.81 +1.4 636,272 - -0.1 636,272 - -0.8 636,536 - RR42_RS02855 0.25 -0.6 636,688 - RR42_RS02855 0.37 +0.5 636,789 + RR42_RS02855 0.44 +1.6 636,790 - RR42_RS02855 0.44 -0.0 636,790 - RR42_RS02855 0.44 +1.4 636,790 - RR42_RS02855 0.44 -0.4 636,838 - RR42_RS02855 0.48 +0.2 636,931 - RR42_RS02855 0.55 -0.3 637,005 - RR42_RS02855 0.60 +1.0 637,005 - RR42_RS02855 0.60 +0.9 637,060 - RR42_RS02855 0.64 +1.0 637,060 - RR42_RS02855 0.64 +0.6 637,069 - RR42_RS02855 0.65 +1.0 637,069 - RR42_RS02855 0.65 -3.0 637,475 - +0.2 637,519 - -0.8 637,519 - -2.2 637,519 - -0.4 637,634 - +1.0 637,634 - +0.7 637,639 + -0.0 637,640 - +0.0 637,640 - +1.5 637,640 - -0.2 637,640 - -1.1 637,763 + RR42_RS02860 0.33 +1.7 637,960 - RR42_RS02860 0.85 -1.4 637,960 - RR42_RS02860 0.85 +0.0 638,106 + +1.4 638,107 - +1.5 638,107 - -0.1 638,107 - -2.2 638,107 - +0.4 638,110 - +0.4 638,110 - +0.4 638,110 - +0.2 638,110 - -0.0 638,110 - +0.2 638,143 + +1.0 638,144 - -1.1 638,144 - +2.0 638,413 + RR42_RS02865 0.16 -0.4 638,413 + RR42_RS02865 0.16 -1.8 638,413 + RR42_RS02865 0.16 -0.0 638,413 + RR42_RS02865 0.16 +0.7 638,414 - RR42_RS02865 0.16 +1.1 638,419 + RR42_RS02865 0.16 -1.2
Or see this region's nucleotide sequence