Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02580 and RR42_RS02585 are separated by 11 nucleotides RR42_RS02585 and RR42_RS02590 overlap by 4 nucleotides RR42_RS02590 and RR42_RS02595 are separated by 145 nucleotides
RR42_RS02580: RR42_RS02580 - amino acid ABC transporter permease, at 580,117 to 580,845
_RS02580
RR42_RS02585: RR42_RS02585 - amino acid ABC transporter permease, at 580,857 to 581,540
_RS02585
RR42_RS02590: RR42_RS02590 - amino acid ABC transporter ATP-binding protein, at 581,537 to 582,271
_RS02590
RR42_RS02595: RR42_RS02595 - glutamine amidotransferase, at 582,417 to 583,247
_RS02595
Position (kb) 581
582
583 Strain fitness (log2 ratio) -2
-1
0
1
2 at 580.570 kb on + strand, within RR42_RS02580 at 580.571 kb on - strand, within RR42_RS02580 at 580.576 kb on + strand, within RR42_RS02580 at 580.577 kb on - strand, within RR42_RS02580 at 580.577 kb on - strand, within RR42_RS02580 at 580.583 kb on + strand, within RR42_RS02580 at 580.583 kb on + strand, within RR42_RS02580 at 580.714 kb on + strand, within RR42_RS02580 at 580.714 kb on + strand, within RR42_RS02580 at 580.715 kb on - strand, within RR42_RS02580 at 580.729 kb on + strand, within RR42_RS02580 at 580.759 kb on + strand, within RR42_RS02580 at 580.760 kb on - strand, within RR42_RS02580 at 580.760 kb on - strand, within RR42_RS02580 at 580.760 kb on - strand, within RR42_RS02580 at 580.843 kb on + strand at 580.843 kb on + strand at 580.843 kb on + strand at 580.844 kb on - strand at 580.844 kb on - strand at 580.844 kb on - strand at 580.863 kb on + strand at 580.863 kb on + strand at 580.863 kb on + strand at 580.863 kb on + strand at 580.864 kb on - strand at 580.864 kb on - strand at 580.864 kb on - strand at 580.864 kb on - strand at 580.864 kb on - strand at 580.865 kb on + strand at 580.865 kb on + strand at 580.865 kb on + strand at 580.865 kb on + strand at 580.865 kb on + strand at 580.865 kb on + strand at 580.866 kb on - strand at 580.866 kb on - strand at 580.866 kb on - strand at 580.866 kb on - strand at 580.866 kb on - strand at 580.866 kb on - strand at 580.866 kb on - strand at 580.866 kb on - strand at 581.037 kb on + strand, within RR42_RS02585 at 581.037 kb on + strand, within RR42_RS02585 at 581.037 kb on + strand, within RR42_RS02585 at 581.038 kb on - strand, within RR42_RS02585 at 581.038 kb on - strand, within RR42_RS02585 at 581.193 kb on + strand, within RR42_RS02585 at 581.194 kb on - strand, within RR42_RS02585 at 581.194 kb on - strand, within RR42_RS02585 at 581.196 kb on + strand, within RR42_RS02585 at 581.197 kb on - strand, within RR42_RS02585 at 581.197 kb on - strand, within RR42_RS02585 at 581.274 kb on + strand, within RR42_RS02585 at 581.367 kb on - strand, within RR42_RS02585 at 581.379 kb on + strand, within RR42_RS02585 at 581.379 kb on + strand, within RR42_RS02585 at 581.379 kb on + strand, within RR42_RS02585 at 581.380 kb on - strand, within RR42_RS02585 at 581.380 kb on - strand, within RR42_RS02585 at 581.380 kb on - strand, within RR42_RS02585 at 581.380 kb on - strand, within RR42_RS02585 at 581.416 kb on - strand, within RR42_RS02585 at 581.475 kb on + strand at 581.476 kb on - strand at 581.476 kb on - strand at 581.483 kb on + strand at 581.536 kb on + strand at 581.536 kb on + strand at 581.536 kb on + strand at 581.568 kb on - strand at 581.568 kb on - strand at 581.894 kb on + strand, within RR42_RS02590 at 581.895 kb on - strand, within RR42_RS02590 at 582.361 kb on + strand at 582.361 kb on + strand at 582.361 kb on + strand at 582.361 kb on + strand at 582.361 kb on + strand at 582.361 kb on + strand at 582.362 kb on - strand at 582.362 kb on - strand at 582.362 kb on - strand at 582.362 kb on - strand at 582.362 kb on - strand at 582.673 kb on + strand, within RR42_RS02595 at 582.673 kb on + strand, within RR42_RS02595 at 582.673 kb on + strand, within RR42_RS02595 at 582.673 kb on + strand, within RR42_RS02595 at 582.673 kb on + strand, within RR42_RS02595 at 582.674 kb on - strand, within RR42_RS02595 at 582.674 kb on - strand, within RR42_RS02595 at 582.674 kb on - strand, within RR42_RS02595 at 582.674 kb on - strand, within RR42_RS02595 at 582.677 kb on - strand, within RR42_RS02595 at 582.727 kb on + strand, within RR42_RS02595 at 582.727 kb on + strand, within RR42_RS02595 at 582.727 kb on + strand, within RR42_RS02595 at 582.728 kb on - strand, within RR42_RS02595 at 582.728 kb on - strand, within RR42_RS02595 at 582.883 kb on + strand, within RR42_RS02595 at 582.883 kb on + strand, within RR42_RS02595 at 582.883 kb on + strand, within RR42_RS02595 at 582.884 kb on - strand, within RR42_RS02595 at 583.016 kb on + strand, within RR42_RS02595
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 580,570 + RR42_RS02580 0.62 -0.0 580,571 - RR42_RS02580 0.62 -0.2 580,576 + RR42_RS02580 0.63 -0.9 580,577 - RR42_RS02580 0.63 +0.8 580,577 - RR42_RS02580 0.63 +0.3 580,583 + RR42_RS02580 0.64 +0.8 580,583 + RR42_RS02580 0.64 +0.0 580,714 + RR42_RS02580 0.82 +1.2 580,714 + RR42_RS02580 0.82 -0.1 580,715 - RR42_RS02580 0.82 -0.2 580,729 + RR42_RS02580 0.84 -0.9 580,759 + RR42_RS02580 0.88 -2.3 580,760 - RR42_RS02580 0.88 +0.6 580,760 - RR42_RS02580 0.88 -0.6 580,760 - RR42_RS02580 0.88 +0.2 580,843 + -0.9 580,843 + -0.4 580,843 + +0.6 580,844 - +1.0 580,844 - +0.7 580,844 - -0.3 580,863 + -0.5 580,863 + -0.2 580,863 + -2.4 580,863 + -0.3 580,864 - -1.4 580,864 - -0.4 580,864 - -0.6 580,864 - +0.4 580,864 - +1.2 580,865 + -0.3 580,865 + +0.0 580,865 + -0.2 580,865 + -0.9 580,865 + -1.7 580,865 + -0.2 580,866 - +0.2 580,866 - +1.2 580,866 - -0.8 580,866 - -0.1 580,866 - -1.3 580,866 - +0.3 580,866 - -0.2 580,866 - -0.2 581,037 + RR42_RS02585 0.26 -0.4 581,037 + RR42_RS02585 0.26 +0.2 581,037 + RR42_RS02585 0.26 +0.3 581,038 - RR42_RS02585 0.26 -0.5 581,038 - RR42_RS02585 0.26 -0.1 581,193 + RR42_RS02585 0.49 -0.5 581,194 - RR42_RS02585 0.49 -1.1 581,194 - RR42_RS02585 0.49 +0.4 581,196 + RR42_RS02585 0.50 -0.2 581,197 - RR42_RS02585 0.50 -0.1 581,197 - RR42_RS02585 0.50 +0.1 581,274 + RR42_RS02585 0.61 +0.2 581,367 - RR42_RS02585 0.75 +0.6 581,379 + RR42_RS02585 0.76 -1.1 581,379 + RR42_RS02585 0.76 +0.2 581,379 + RR42_RS02585 0.76 +0.5 581,380 - RR42_RS02585 0.76 +0.2 581,380 - RR42_RS02585 0.76 +1.9 581,380 - RR42_RS02585 0.76 +0.1 581,380 - RR42_RS02585 0.76 -0.2 581,416 - RR42_RS02585 0.82 -0.1 581,475 + -0.7 581,476 - -1.0 581,476 - +0.1 581,483 + +1.8 581,536 + +0.0 581,536 + +0.3 581,536 + -0.3 581,568 - -0.2 581,568 - -1.0 581,894 + RR42_RS02590 0.49 -0.1 581,895 - RR42_RS02590 0.49 -1.4 582,361 + -0.8 582,361 + -0.2 582,361 + +0.3 582,361 + -1.1 582,361 + -0.2 582,361 + +0.1 582,362 - -0.3 582,362 - -0.1 582,362 - -0.2 582,362 - +0.7 582,362 - +0.0 582,673 + RR42_RS02595 0.31 +0.8 582,673 + RR42_RS02595 0.31 +0.8 582,673 + RR42_RS02595 0.31 -0.4 582,673 + RR42_RS02595 0.31 -0.6 582,673 + RR42_RS02595 0.31 +0.3 582,674 - RR42_RS02595 0.31 -0.6 582,674 - RR42_RS02595 0.31 -0.1 582,674 - RR42_RS02595 0.31 -0.3 582,674 - RR42_RS02595 0.31 -0.7 582,677 - RR42_RS02595 0.31 +0.2 582,727 + RR42_RS02595 0.37 -1.0 582,727 + RR42_RS02595 0.37 -0.3 582,727 + RR42_RS02595 0.37 +0.1 582,728 - RR42_RS02595 0.37 -1.3 582,728 - RR42_RS02595 0.37 +0.3 582,883 + RR42_RS02595 0.56 -0.1 582,883 + RR42_RS02595 0.56 -0.3 582,883 + RR42_RS02595 0.56 +1.3 582,884 - RR42_RS02595 0.56 -0.3 583,016 + RR42_RS02595 0.72 -1.2
Or see this region's nucleotide sequence