Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02380 and RR42_RS02385 are separated by 70 nucleotides RR42_RS02385 and RR42_RS02390 are separated by 170 nucleotides RR42_RS02390 and RR42_RS02395 are separated by 52 nucleotides RR42_RS02395 and RR42_RS02400 are separated by 42 nucleotides
RR42_RS02380: RR42_RS02380 - FAD-linked oxidase, at 533,553 to 537,527
_RS02380
RR42_RS02385: RR42_RS02385 - hydrolase, at 537,598 to 538,032
_RS02385
RR42_RS02390: RR42_RS02390 - ABC transporter ATP-binding protein, at 538,203 to 540,086
_RS02390
RR42_RS02395: RR42_RS02395 - hypothetical protein, at 540,139 to 540,552
_RS02395
RR42_RS02400: RR42_RS02400 - ubiquinone biosynthesis methyltransferase UbiE, at 540,595 to 541,326
_RS02400
Position (kb) 538
539
540
541 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 537.396 kb on + strand at 537.859 kb on - strand, within RR42_RS02385 at 537.882 kb on + strand, within RR42_RS02385 at 537.883 kb on - strand, within RR42_RS02385 at 538.130 kb on - strand at 538.130 kb on - strand at 538.311 kb on + strand at 538.311 kb on + strand at 538.312 kb on - strand at 538.386 kb on + strand at 538.387 kb on - strand at 538.387 kb on - strand at 538.388 kb on + strand at 538.388 kb on + strand at 538.389 kb on - strand at 538.389 kb on - strand at 538.389 kb on - strand at 538.426 kb on - strand, within RR42_RS02390 at 538.449 kb on + strand, within RR42_RS02390 at 538.449 kb on + strand, within RR42_RS02390 at 538.450 kb on - strand, within RR42_RS02390 at 538.450 kb on - strand, within RR42_RS02390 at 538.502 kb on + strand, within RR42_RS02390 at 538.503 kb on - strand, within RR42_RS02390 at 538.516 kb on + strand, within RR42_RS02390 at 538.517 kb on - strand, within RR42_RS02390 at 538.517 kb on - strand, within RR42_RS02390 at 538.517 kb on - strand, within RR42_RS02390 at 538.527 kb on + strand, within RR42_RS02390 at 538.527 kb on + strand, within RR42_RS02390 at 538.528 kb on - strand, within RR42_RS02390 at 538.530 kb on + strand, within RR42_RS02390 at 538.530 kb on + strand, within RR42_RS02390 at 538.530 kb on + strand, within RR42_RS02390 at 538.530 kb on + strand, within RR42_RS02390 at 538.531 kb on - strand, within RR42_RS02390 at 538.608 kb on + strand, within RR42_RS02390 at 538.608 kb on + strand, within RR42_RS02390 at 538.608 kb on + strand, within RR42_RS02390 at 538.612 kb on - strand, within RR42_RS02390 at 538.618 kb on + strand, within RR42_RS02390 at 538.619 kb on - strand, within RR42_RS02390 at 538.619 kb on - strand, within RR42_RS02390 at 538.815 kb on + strand, within RR42_RS02390 at 538.816 kb on - strand, within RR42_RS02390 at 538.817 kb on + strand, within RR42_RS02390 at 538.817 kb on + strand, within RR42_RS02390 at 538.817 kb on + strand, within RR42_RS02390 at 538.817 kb on + strand, within RR42_RS02390 at 538.817 kb on + strand, within RR42_RS02390 at 538.818 kb on - strand, within RR42_RS02390 at 538.818 kb on - strand, within RR42_RS02390 at 538.818 kb on - strand, within RR42_RS02390 at 539.059 kb on - strand, within RR42_RS02390 at 539.059 kb on - strand, within RR42_RS02390 at 539.060 kb on + strand, within RR42_RS02390 at 539.060 kb on + strand, within RR42_RS02390 at 539.139 kb on + strand, within RR42_RS02390 at 539.139 kb on + strand, within RR42_RS02390 at 539.232 kb on + strand, within RR42_RS02390 at 539.232 kb on + strand, within RR42_RS02390 at 539.232 kb on + strand, within RR42_RS02390 at 539.291 kb on + strand, within RR42_RS02390 at 539.292 kb on - strand, within RR42_RS02390 at 539.587 kb on + strand, within RR42_RS02390 at 539.588 kb on - strand, within RR42_RS02390 at 539.588 kb on - strand, within RR42_RS02390 at 539.634 kb on + strand, within RR42_RS02390 at 539.901 kb on + strand at 539.902 kb on - strand at 540.084 kb on + strand at 540.253 kb on + strand, within RR42_RS02395 at 540.367 kb on + strand, within RR42_RS02395 at 540.367 kb on + strand, within RR42_RS02395 at 540.367 kb on + strand, within RR42_RS02395 at 540.367 kb on + strand, within RR42_RS02395 at 540.402 kb on + strand, within RR42_RS02395 at 540.412 kb on + strand, within RR42_RS02395 at 540.470 kb on - strand, within RR42_RS02395
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 537,396 + -1.2 537,859 - RR42_RS02385 0.60 -0.5 537,882 + RR42_RS02385 0.65 -0.2 537,883 - RR42_RS02385 0.66 +1.1 538,130 - +0.8 538,130 - -0.1 538,311 + +0.0 538,311 + -0.2 538,312 - +0.4 538,386 + -0.0 538,387 - +1.8 538,387 - +0.0 538,388 + -0.2 538,388 + +0.2 538,389 - -0.5 538,389 - -0.9 538,389 - -0.5 538,426 - RR42_RS02390 0.12 -0.3 538,449 + RR42_RS02390 0.13 +0.1 538,449 + RR42_RS02390 0.13 +0.7 538,450 - RR42_RS02390 0.13 -0.6 538,450 - RR42_RS02390 0.13 +0.3 538,502 + RR42_RS02390 0.16 -0.8 538,503 - RR42_RS02390 0.16 +0.4 538,516 + RR42_RS02390 0.17 -0.2 538,517 - RR42_RS02390 0.17 -1.3 538,517 - RR42_RS02390 0.17 +0.3 538,517 - RR42_RS02390 0.17 -0.0 538,527 + RR42_RS02390 0.17 -0.5 538,527 + RR42_RS02390 0.17 +0.1 538,528 - RR42_RS02390 0.17 -0.0 538,530 + RR42_RS02390 0.17 +0.6 538,530 + RR42_RS02390 0.17 +0.0 538,530 + RR42_RS02390 0.17 -1.4 538,530 + RR42_RS02390 0.17 -0.5 538,531 - RR42_RS02390 0.17 +1.1 538,608 + RR42_RS02390 0.21 +0.3 538,608 + RR42_RS02390 0.21 +0.1 538,608 + RR42_RS02390 0.21 -0.7 538,612 - RR42_RS02390 0.22 -1.2 538,618 + RR42_RS02390 0.22 +0.1 538,619 - RR42_RS02390 0.22 -0.7 538,619 - RR42_RS02390 0.22 -0.1 538,815 + RR42_RS02390 0.32 -0.5 538,816 - RR42_RS02390 0.33 +0.5 538,817 + RR42_RS02390 0.33 +0.8 538,817 + RR42_RS02390 0.33 +0.1 538,817 + RR42_RS02390 0.33 +0.4 538,817 + RR42_RS02390 0.33 +0.7 538,817 + RR42_RS02390 0.33 +1.5 538,818 - RR42_RS02390 0.33 -0.6 538,818 - RR42_RS02390 0.33 +0.5 538,818 - RR42_RS02390 0.33 +0.1 539,059 - RR42_RS02390 0.45 -0.7 539,059 - RR42_RS02390 0.45 -0.2 539,060 + RR42_RS02390 0.45 -3.2 539,060 + RR42_RS02390 0.45 -1.1 539,139 + RR42_RS02390 0.50 +0.3 539,139 + RR42_RS02390 0.50 -0.6 539,232 + RR42_RS02390 0.55 -0.1 539,232 + RR42_RS02390 0.55 -0.6 539,232 + RR42_RS02390 0.55 +2.9 539,291 + RR42_RS02390 0.58 +1.1 539,292 - RR42_RS02390 0.58 +0.5 539,587 + RR42_RS02390 0.73 -0.0 539,588 - RR42_RS02390 0.74 +0.1 539,588 - RR42_RS02390 0.74 +0.1 539,634 + RR42_RS02390 0.76 +0.4 539,901 + +2.2 539,902 - +0.3 540,084 + -0.8 540,253 + RR42_RS02395 0.28 +0.1 540,367 + RR42_RS02395 0.55 -0.8 540,367 + RR42_RS02395 0.55 +0.4 540,367 + RR42_RS02395 0.55 -1.5 540,367 + RR42_RS02395 0.55 -0.2 540,402 + RR42_RS02395 0.64 +0.1 540,412 + RR42_RS02395 0.66 -0.0 540,470 - RR42_RS02395 0.80 -0.3
Or see this region's nucleotide sequence