Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01670 and RR42_RS01675 are separated by 169 nucleotides RR42_RS01675 and RR42_RS01680 are separated by 13 nucleotides RR42_RS01680 and RR42_RS01685 are separated by 72 nucleotides RR42_RS01685 and RR42_RS01690 are separated by 97 nucleotides
RR42_RS01670: RR42_RS01670 - 23S rRNA methyltransferase, at 380,692 to 381,885
_RS01670
RR42_RS01675: RR42_RS01675 - hypothetical protein, at 382,055 to 382,360
_RS01675
RR42_RS01680: RR42_RS01680 - cytochrome C550, at 382,374 to 383,582
_RS01680
RR42_RS01685: RR42_RS01685 - alpha/beta hydrolase, at 383,655 to 384,299
_RS01685
RR42_RS01690: RR42_RS01690 - ferredoxin, at 384,397 to 384,717
_RS01690
Position (kb)
382
383
384 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 381.423 kb on - strand, within RR42_RS01670 at 381.539 kb on + strand, within RR42_RS01670 at 381.539 kb on + strand, within RR42_RS01670 at 381.539 kb on + strand, within RR42_RS01670 at 381.540 kb on - strand, within RR42_RS01670 at 381.540 kb on - strand, within RR42_RS01670 at 381.540 kb on - strand, within RR42_RS01670 at 381.641 kb on - strand, within RR42_RS01670 at 381.821 kb on + strand at 381.957 kb on + strand at 381.957 kb on + strand at 382.039 kb on + strand at 382.057 kb on - strand at 382.057 kb on - strand at 382.376 kb on - strand at 382.376 kb on - strand at 382.376 kb on - strand at 382.403 kb on + strand at 382.600 kb on + strand, within RR42_RS01680 at 382.600 kb on + strand, within RR42_RS01680 at 382.600 kb on + strand, within RR42_RS01680 at 382.601 kb on - strand, within RR42_RS01680 at 382.601 kb on - strand, within RR42_RS01680 at 382.601 kb on - strand, within RR42_RS01680 at 382.648 kb on + strand, within RR42_RS01680 at 382.648 kb on + strand, within RR42_RS01680 at 382.648 kb on + strand, within RR42_RS01680 at 382.648 kb on + strand, within RR42_RS01680 at 382.649 kb on - strand, within RR42_RS01680 at 382.649 kb on - strand, within RR42_RS01680 at 382.649 kb on - strand, within RR42_RS01680 at 382.718 kb on - strand, within RR42_RS01680 at 382.847 kb on - strand, within RR42_RS01680 at 382.900 kb on + strand, within RR42_RS01680 at 382.900 kb on + strand, within RR42_RS01680 at 382.900 kb on + strand, within RR42_RS01680 at 382.901 kb on - strand, within RR42_RS01680 at 382.901 kb on - strand, within RR42_RS01680 at 382.901 kb on - strand, within RR42_RS01680 at 382.939 kb on + strand, within RR42_RS01680 at 382.940 kb on - strand, within RR42_RS01680 at 382.960 kb on + strand, within RR42_RS01680 at 382.960 kb on + strand, within RR42_RS01680 at 382.960 kb on + strand, within RR42_RS01680 at 382.969 kb on + strand, within RR42_RS01680 at 382.970 kb on - strand, within RR42_RS01680 at 383.341 kb on + strand, within RR42_RS01680 at 383.342 kb on - strand, within RR42_RS01680 at 383.573 kb on + strand at 384.275 kb on + strand at 384.276 kb on - strand at 384.280 kb on - strand at 384.280 kb on - strand at 384.280 kb on - strand at 384.287 kb on + strand at 384.488 kb on + strand, within RR42_RS01690 at 384.489 kb on - strand, within RR42_RS01690 at 384.506 kb on + strand, within RR42_RS01690
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 381,423 - RR42_RS01670 0.61 +0.8 381,539 + RR42_RS01670 0.71 +0.1 381,539 + RR42_RS01670 0.71 +0.2 381,539 + RR42_RS01670 0.71 -0.2 381,540 - RR42_RS01670 0.71 -0.2 381,540 - RR42_RS01670 0.71 -0.3 381,540 - RR42_RS01670 0.71 +0.9 381,641 - RR42_RS01670 0.79 -0.1 381,821 + +0.1 381,957 + +0.1 381,957 + -0.8 382,039 + +0.0 382,057 - -0.3 382,057 - -0.2 382,376 - -0.2 382,376 - +0.3 382,376 - -0.4 382,403 + -0.9 382,600 + RR42_RS01680 0.19 -0.8 382,600 + RR42_RS01680 0.19 -1.3 382,600 + RR42_RS01680 0.19 -0.3 382,601 - RR42_RS01680 0.19 -1.1 382,601 - RR42_RS01680 0.19 -0.7 382,601 - RR42_RS01680 0.19 -1.5 382,648 + RR42_RS01680 0.23 -1.5 382,648 + RR42_RS01680 0.23 -2.2 382,648 + RR42_RS01680 0.23 -0.5 382,648 + RR42_RS01680 0.23 -0.2 382,649 - RR42_RS01680 0.23 -2.1 382,649 - RR42_RS01680 0.23 -1.0 382,649 - RR42_RS01680 0.23 -0.6 382,718 - RR42_RS01680 0.28 -0.4 382,847 - RR42_RS01680 0.39 -2.5 382,900 + RR42_RS01680 0.44 -0.9 382,900 + RR42_RS01680 0.44 -1.6 382,900 + RR42_RS01680 0.44 -1.1 382,901 - RR42_RS01680 0.44 -1.5 382,901 - RR42_RS01680 0.44 -1.7 382,901 - RR42_RS01680 0.44 -1.2 382,939 + RR42_RS01680 0.47 -2.4 382,940 - RR42_RS01680 0.47 -1.7 382,960 + RR42_RS01680 0.48 -1.8 382,960 + RR42_RS01680 0.48 -0.6 382,960 + RR42_RS01680 0.48 -0.8 382,969 + RR42_RS01680 0.49 -2.2 382,970 - RR42_RS01680 0.49 -1.2 383,341 + RR42_RS01680 0.80 -0.9 383,342 - RR42_RS01680 0.80 -2.1 383,573 + -2.1 384,275 + -1.0 384,276 - +0.5 384,280 - -0.2 384,280 - +0.1 384,280 - +0.4 384,287 + -2.2 384,488 + RR42_RS01690 0.28 -4.2 384,489 - RR42_RS01690 0.29 -0.7 384,506 + RR42_RS01690 0.34 -0.6
Or see this region's nucleotide sequence