Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01395 and RR42_RS01400 are separated by 22 nucleotides RR42_RS01400 and RR42_RS01405 are separated by 153 nucleotides RR42_RS01405 and RR42_RS01410 are separated by 104 nucleotides
RR42_RS01395: RR42_RS01395 - twitching motility protein PilT, at 314,708 to 315,475
_RS01395
RR42_RS01400: RR42_RS01400 - 5-formyltetrahydrofolate cyclo-ligase, at 315,498 to 316,049
_RS01400
RR42_RS01405: RR42_RS01405 - lytic transglycosylase, at 316,203 to 318,155
_RS01405
RR42_RS01410: RR42_RS01410 - NAD-dependent dehydratase, at 318,260 to 319,285
_RS01410
Position (kb) 316
317
318
319 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 315.430 kb on + strand at 315.430 kb on + strand at 315.430 kb on + strand at 315.430 kb on + strand at 315.431 kb on - strand at 315.431 kb on - strand at 315.473 kb on + strand at 315.473 kb on + strand at 315.473 kb on + strand at 315.473 kb on + strand at 315.474 kb on - strand at 315.644 kb on - strand, within RR42_RS01400 at 315.674 kb on + strand, within RR42_RS01400 at 315.675 kb on - strand, within RR42_RS01400 at 315.708 kb on + strand, within RR42_RS01400 at 315.709 kb on - strand, within RR42_RS01400 at 315.754 kb on + strand, within RR42_RS01400 at 315.755 kb on - strand, within RR42_RS01400 at 315.913 kb on + strand, within RR42_RS01400 at 315.914 kb on - strand, within RR42_RS01400 at 315.914 kb on - strand, within RR42_RS01400 at 316.119 kb on + strand at 316.120 kb on - strand at 316.216 kb on + strand at 316.216 kb on + strand at 316.217 kb on - strand at 316.219 kb on - strand at 316.219 kb on - strand at 316.219 kb on - strand at 316.282 kb on + strand at 316.283 kb on - strand at 316.431 kb on + strand, within RR42_RS01405 at 316.446 kb on + strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.455 kb on + strand, within RR42_RS01405 at 316.455 kb on + strand, within RR42_RS01405 at 316.545 kb on + strand, within RR42_RS01405 at 316.546 kb on - strand, within RR42_RS01405 at 316.678 kb on - strand, within RR42_RS01405 at 316.752 kb on + strand, within RR42_RS01405 at 316.756 kb on - strand, within RR42_RS01405 at 316.785 kb on + strand, within RR42_RS01405 at 316.857 kb on + strand, within RR42_RS01405 at 316.857 kb on + strand, within RR42_RS01405 at 316.857 kb on + strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.972 kb on - strand, within RR42_RS01405 at 317.077 kb on - strand, within RR42_RS01405 at 317.080 kb on - strand, within RR42_RS01405 at 317.238 kb on + strand, within RR42_RS01405 at 317.238 kb on + strand, within RR42_RS01405 at 317.238 kb on + strand, within RR42_RS01405 at 317.239 kb on - strand, within RR42_RS01405 at 317.239 kb on - strand, within RR42_RS01405 at 317.239 kb on - strand, within RR42_RS01405 at 317.245 kb on - strand, within RR42_RS01405 at 317.325 kb on + strand, within RR42_RS01405 at 317.325 kb on + strand, within RR42_RS01405 at 317.325 kb on + strand, within RR42_RS01405 at 317.449 kb on - strand, within RR42_RS01405 at 317.449 kb on - strand, within RR42_RS01405 at 317.538 kb on + strand, within RR42_RS01405 at 317.688 kb on + strand, within RR42_RS01405 at 317.689 kb on - strand, within RR42_RS01405 at 317.850 kb on - strand, within RR42_RS01405 at 317.868 kb on + strand, within RR42_RS01405 at 317.868 kb on + strand, within RR42_RS01405 at 317.868 kb on + strand, within RR42_RS01405 at 317.868 kb on + strand, within RR42_RS01405 at 317.868 kb on + strand, within RR42_RS01405 at 317.868 kb on + strand, within RR42_RS01405 at 317.869 kb on - strand, within RR42_RS01405 at 317.869 kb on - strand, within RR42_RS01405 at 317.869 kb on - strand, within RR42_RS01405 at 317.869 kb on - strand, within RR42_RS01405 at 317.869 kb on - strand, within RR42_RS01405 at 317.869 kb on - strand, within RR42_RS01405 at 317.869 kb on - strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.871 kb on - strand, within RR42_RS01405 at 317.871 kb on - strand, within RR42_RS01405 at 317.871 kb on - strand, within RR42_RS01405 at 317.876 kb on + strand, within RR42_RS01405 at 317.876 kb on + strand, within RR42_RS01405 at 317.877 kb on - strand, within RR42_RS01405 at 317.926 kb on - strand, within RR42_RS01405 at 318.030 kb on + strand at 318.030 kb on + strand at 318.031 kb on - strand at 318.031 kb on - strand at 318.060 kb on + strand at 318.060 kb on + strand at 318.060 kb on + strand at 318.060 kb on + strand at 318.060 kb on + strand at 318.060 kb on + strand at 318.060 kb on + strand at 318.063 kb on + strand at 318.063 kb on + strand at 318.063 kb on + strand at 318.063 kb on + strand at 318.064 kb on - strand at 318.064 kb on - strand at 318.064 kb on - strand at 318.064 kb on - strand at 318.064 kb on - strand at 318.162 kb on - strand at 318.279 kb on + strand at 318.714 kb on - strand, within RR42_RS01410 at 318.714 kb on - strand, within RR42_RS01410 at 318.893 kb on + strand, within RR42_RS01410 at 318.893 kb on + strand, within RR42_RS01410 at 318.893 kb on + strand, within RR42_RS01410 at 318.894 kb on - strand, within RR42_RS01410 at 318.894 kb on - strand, within RR42_RS01410 at 318.959 kb on + strand, within RR42_RS01410 at 318.983 kb on + strand, within RR42_RS01410 at 318.984 kb on - strand, within RR42_RS01410 at 318.984 kb on - strand, within RR42_RS01410 at 318.985 kb on + strand, within RR42_RS01410 at 318.985 kb on + strand, within RR42_RS01410 at 318.985 kb on + strand, within RR42_RS01410 at 318.985 kb on + strand, within RR42_RS01410 at 318.986 kb on - strand, within RR42_RS01410 at 318.986 kb on - strand, within RR42_RS01410 at 318.986 kb on - strand, within RR42_RS01410
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 315,430 + -0.7 315,430 + -0.1 315,430 + -0.3 315,430 + +0.3 315,431 - -1.6 315,431 - -0.2 315,473 + +0.4 315,473 + +0.3 315,473 + -1.4 315,473 + -0.3 315,474 - -0.1 315,644 - RR42_RS01400 0.26 -0.1 315,674 + RR42_RS01400 0.32 +0.5 315,675 - RR42_RS01400 0.32 +0.2 315,708 + RR42_RS01400 0.38 +0.1 315,709 - RR42_RS01400 0.38 +0.1 315,754 + RR42_RS01400 0.46 +0.4 315,755 - RR42_RS01400 0.47 -0.5 315,913 + RR42_RS01400 0.75 -0.1 315,914 - RR42_RS01400 0.75 +0.6 315,914 - RR42_RS01400 0.75 -0.1 316,119 + +0.4 316,120 - -0.6 316,216 + -1.6 316,216 + -0.6 316,217 - -0.4 316,219 - -1.5 316,219 - -1.3 316,219 - -2.5 316,282 + -1.3 316,283 - -1.6 316,431 + RR42_RS01405 0.12 -1.6 316,446 + RR42_RS01405 0.12 -0.1 316,447 - RR42_RS01405 0.12 -1.3 316,447 - RR42_RS01405 0.12 -1.3 316,447 - RR42_RS01405 0.12 -0.7 316,447 - RR42_RS01405 0.12 -1.5 316,455 + RR42_RS01405 0.13 +1.3 316,455 + RR42_RS01405 0.13 -2.6 316,545 + RR42_RS01405 0.18 -1.4 316,546 - RR42_RS01405 0.18 -1.4 316,678 - RR42_RS01405 0.24 -1.2 316,752 + RR42_RS01405 0.28 -0.5 316,756 - RR42_RS01405 0.28 -2.3 316,785 + RR42_RS01405 0.30 -1.8 316,857 + RR42_RS01405 0.33 -0.7 316,857 + RR42_RS01405 0.33 -1.9 316,857 + RR42_RS01405 0.33 -2.5 316,858 - RR42_RS01405 0.34 -1.2 316,858 - RR42_RS01405 0.34 -1.3 316,858 - RR42_RS01405 0.34 -2.5 316,858 - RR42_RS01405 0.34 -1.6 316,858 - RR42_RS01405 0.34 -1.9 316,858 - RR42_RS01405 0.34 -1.0 316,972 - RR42_RS01405 0.39 -1.9 317,077 - RR42_RS01405 0.45 -2.4 317,080 - RR42_RS01405 0.45 -2.1 317,238 + RR42_RS01405 0.53 -1.5 317,238 + RR42_RS01405 0.53 -0.5 317,238 + RR42_RS01405 0.53 -2.1 317,239 - RR42_RS01405 0.53 -1.2 317,239 - RR42_RS01405 0.53 -0.2 317,239 - RR42_RS01405 0.53 -0.8 317,245 - RR42_RS01405 0.53 -2.0 317,325 + RR42_RS01405 0.57 -0.5 317,325 + RR42_RS01405 0.57 -2.0 317,325 + RR42_RS01405 0.57 -3.5 317,449 - RR42_RS01405 0.64 -0.7 317,449 - RR42_RS01405 0.64 -1.2 317,538 + RR42_RS01405 0.68 -1.5 317,688 + RR42_RS01405 0.76 -2.1 317,689 - RR42_RS01405 0.76 -1.1 317,850 - RR42_RS01405 0.84 -2.5 317,868 + RR42_RS01405 0.85 -1.0 317,868 + RR42_RS01405 0.85 -0.6 317,868 + RR42_RS01405 0.85 -1.3 317,868 + RR42_RS01405 0.85 -2.3 317,868 + RR42_RS01405 0.85 -0.9 317,868 + RR42_RS01405 0.85 -1.8 317,869 - RR42_RS01405 0.85 -1.4 317,869 - RR42_RS01405 0.85 -1.0 317,869 - RR42_RS01405 0.85 -3.6 317,869 - RR42_RS01405 0.85 -2.0 317,869 - RR42_RS01405 0.85 -0.2 317,869 - RR42_RS01405 0.85 -1.7 317,869 - RR42_RS01405 0.85 -4.1 317,870 + RR42_RS01405 0.85 -0.5 317,870 + RR42_RS01405 0.85 -0.5 317,870 + RR42_RS01405 0.85 -0.6 317,870 + RR42_RS01405 0.85 +0.0 317,870 + RR42_RS01405 0.85 -1.7 317,870 + RR42_RS01405 0.85 -0.1 317,870 + RR42_RS01405 0.85 -1.4 317,870 + RR42_RS01405 0.85 -1.0 317,871 - RR42_RS01405 0.85 -2.4 317,871 - RR42_RS01405 0.85 -1.5 317,871 - RR42_RS01405 0.85 -1.3 317,876 + RR42_RS01405 0.86 -1.4 317,876 + RR42_RS01405 0.86 -3.5 317,877 - RR42_RS01405 0.86 -2.7 317,926 - RR42_RS01405 0.88 -1.6 318,030 + -1.5 318,030 + -2.2 318,031 - -1.1 318,031 - -1.3 318,060 + -1.9 318,060 + -1.7 318,060 + -2.9 318,060 + -2.0 318,060 + -0.3 318,060 + -1.1 318,060 + -1.5 318,063 + -1.7 318,063 + -2.2 318,063 + -1.1 318,063 + -0.0 318,064 - -0.6 318,064 - -0.5 318,064 - -1.5 318,064 - -2.5 318,064 - -1.4 318,162 - -1.1 318,279 + -0.6 318,714 - RR42_RS01410 0.44 +0.4 318,714 - RR42_RS01410 0.44 -0.4 318,893 + RR42_RS01410 0.62 +0.0 318,893 + RR42_RS01410 0.62 +0.2 318,893 + RR42_RS01410 0.62 +0.1 318,894 - RR42_RS01410 0.62 -0.3 318,894 - RR42_RS01410 0.62 -0.9 318,959 + RR42_RS01410 0.68 -1.7 318,983 + RR42_RS01410 0.70 -0.2 318,984 - RR42_RS01410 0.71 -0.2 318,984 - RR42_RS01410 0.71 +0.7 318,985 + RR42_RS01410 0.71 +1.3 318,985 + RR42_RS01410 0.71 +0.6 318,985 + RR42_RS01410 0.71 -0.2 318,985 + RR42_RS01410 0.71 +0.9 318,986 - RR42_RS01410 0.71 +0.1 318,986 - RR42_RS01410 0.71 -1.1 318,986 - RR42_RS01410 0.71 +0.8
Or see this region's nucleotide sequence